ABSTRACT
Dental unit waterline system is considered potential source for contamination with Legionella species. The aim of this study was to determine if contamination of a dental unit water line system by Legionella pneumophila serogroup1 in the Mashhad School of Dentistry occurred in 2009. A total of 52 dental units were selected from all clinical departments of the Mashhad School of Dentistry. Samples of water were collected from outlets of water/air spray, high-speed dental hand pieces and water cup fillers. Samples were tested via the ELISA method. At the beginning of the work day, a total of 36.1 percent of dental units were contaminated by Le-gionella pneumophila serogroup 1. Infection control of the dental unit water line system regarding legionella in the Mashhad School of Dentistry is a challenge and engineering controls should be used in contaminated clinics
ABSTRACT
This study was done on 20 patients with endemic schistosomal hepatosplenomegaly and 10 normal controls. Tissue IgS showed significant increase in mucosa, IgG [45%], IgM [55%] and IgA [85%] of cases. The serum IgS showed significant increase in IgG in all cases with mean value of [3360 mg/100 ml +/- 471], the IgA was increased in 60% of cases with mean value of [432 mg/100 ml +/- 173], while IgM was increased in 45% of cases with mean value of [301 mg/100 ml +/- 131]
Subject(s)
Immunoglobulins , Hepatomegaly , SplenomegalyABSTRACT
Human sera [330 from patients with manifestations suggestive trichinosis] were examined for antibodies. The immunologic tests used were counter current immunoelectrophoresis [CEP], enzyme linked immunosorbent assay [ELISA] and immunoflourescence [IF]. Seropositive reactions were demonstrated in 2.7%, 5.4% and 2.4% of the sera by the 3 tests respectively. However, 2.4% of the sera were seropositive by the three tests. It seems that the use of two of these tests is valuable in diagnosis when muscle biopsy is unproductive
ABSTRACT
The counter current immunoelectrophoresis [CEP], the enzyme linked immunosorbent assay [ELISA] and the indirect immunofluorescent [IF] were evaluated in detection of antibodies in naturally infected rats. The test subjects included 10 infected rats as indicated by digestion method of muscle biopsy and 13 rats free from trichinosis. Of the immunodiagnostic tests evaluated, the CEP gave the highest diagnostic accuracy. The ELISA was the least accurate. The high sensitivity and specificity of the CEP for detection of anti-trichinella antibodies suggested that it could be developed as a serodiagnostic test for trichinosis