Analgesic, antioxidant and cytotoxic activity of calotropis gigantean L. Latex

The dried latex of Calotropis gigantean L. was tested for its analgesic, antioxidant and cytotoxic activity in mice. The latex significantly and dose dependently inhibited acetic acid induced writhing by 79.13% and 39.4% at 500 and 250 mg/kg body weight, respectively, comparable to diclofenac sodium. The antioxidant activity was determined qualitatively based on the scavenging activity of 1,1-dipheny 1-2-picryl hydrazyl [DPPH] free radical showing the presence of antioxidant principle. In brine shrimp lethality assay the latex was toxic with LC[50] and LC[90] values of 1.7 micro g/ml and 10 micro g/ ml, respectively. These results suggest that latex possess analgesic and antioxidant activities supporting its traditional uses. However, C. gigantea latex should be used with caution as it also showed toxicity, but more experiments are required in its support

Assessment of phytochemical and pharmacological activites of Anisomeles indica leaves

The leaves of Anisomeles indica was tested for its antioxidant, analgesic, and antidiarrhoeal activities in experimental animals. The extract showed the presence of antioxidant principle qualitatively based on the scavenging activity of 1,1-diphenyl-2-picryl hydrazyl [DPPH] free radical. It showed the presence of antioxidant principle. The extract dose dependently inhibited the acetic acid induced writhing in mice [63.05%, P<0.001 and 37.63% for 500 and 250 mg/kg body weight respectively], the effect was significant comparable to the standard drug Diclofenac Sodium [54.92%, P<0.001] at the dose of 25 mg/kg. The extract of A. indica offered about 1.20 hrs and 3.7 hrs [P<0.001] of the mean latent period for diarrhoeal episode at the doses of 250 and 500 mg/kg body weight which is comparable to standard drug Loperamide. The results tend to suggest that the leaves might possess analgesic, antioxidant and antidiarrhoeal activities or active constituent[s] responsible for the activities and supported its traditional uses

Assessment of phytochemical and pharmacological activities of Leucas zeylanica [L.] R.Br. [Family: Lamiaceae]

The aim of the present study is to investigate the antioxidant, neuropharmacological, analgesic and antimicrobial activities of the EtOH extract of the whole plant of Leucas zeylanica. Phytochemical screening indicated the presence of gums, carbohydrate, reducing sugar, phenols, tannins [gallic acid], cardiac glycosides and saponins. TLC-based antioxidant assay by DPPH free radical revealed positive activity of the extract and in quantitative assay IC[50] value was 62.9 microg/ml. The extract potentiated the phenobarbitone-induced sleeping time in mice in a dose dependent manner. From the study of analgesic activity using the model of acetic acid-induced writhing in mice it was found that the extract exhibited [***p<0.001] writhing inhibition was dose dependent. The extract showed moderate antimicrobial activity

Chemical and biological investigation of kigelia pinnata

The crude ethanolic extract of the stem bark of Kigelia pinnata was evaluated for its antioxidant, cytotoxic and antinociceptive activities. The antioxidant property of the ethanol extract of Kigelia pinnata was assessed by 1, l-diphenyl-2-picryl hydrazyl [DPPH] free radical scavenging assay. The extract showed potential antioxidant activity [IC[50] about 110 micro g/ml], which was comparable to standard drug ascorbic acid [IC [50] at about 3.16 micro g/ml]. Brine Shrimp lethality bioassay indicated its cytotoxic property [LC[50] 100 micro g/ml]. Moreover, it produced significant [P < 0.001] writhing inhibition in acetic acid induced writhing in mice at the dose of 250 and 500 mg/kg body weight respectively, which was comparable to the standard drug diclofenac sodium. The results tend to suggest the antioxidant, cytotoxic and antinociceptive activities of the crude ethanolic extract of Kigelia pinnata

Study of antinociceptive and antioxidant activities of [Hyptis capitata, Litsea glutinosa and Vernonia patula three medicinal plants of Bangladesh

A number of Bangladeshi medicinal plants have been reported to possess antinociceptive and antioxidant activities. Many of which have been used in the preparations of the Unani and Ayurvedic traditional medicine. As a part of our on-going research for exploring the bioactivities of Bangladeshi medicinal plants. Bala [llyptis capitata], Kukur-Chita [Litsea glutinosa], and Bonmulo [Vernonia patula] have been studied for their antinociceptive and antioxidant activity together with the phytochemicals namely, alkaloids, flavonoids. steroids, and tannins. Except for L. glutinosa, the ethanolic extracts of V. patula and H. capitata significantly reduced the acetic acid induced writhing in mice. In DPPH free radical scavenging assay all the extracts showed mild to moderate activity, in which IC[50] of H. capitata and V. patula were > 100 micro g/ml and that of L. glutinosa was > 500 micro g/ml. All the extracts showed positive tests with Dragendorffs. Braemer's, and Liebermann-Burchardt's reagent but gave negative result with Shinoda's reagents, except for V. patula