ABSTRACT
Background and Aim: A peptic ulcer is a breach in the gastric or duodenal mucosa down to the submucosa. There is evidence concerning the role of Reactive Oxygen Species [ROS] in the genesis of such ulcers production of intracellular ROS along mitochondria oxidative phosphorylation [OXPHOS] predisposes the deletion of 4977 bp mtDNA. The aim of the present study was to evaluate the association of 4977 bp mtDNA deletion with peptic ulcer disease
Materials and Methods: In this case-control study included 110 patients with peptic ulcer disease and 110 healthy individuals were compared. Genomic DNAs of the cases and controls were extracted from bioptic tissues. Then, their genotypes were determined by means of Polymerase Chain Reaction [PCR]. Finally, statistical analysis was performed using the MedCalc program
Results: Deletion of 4977 bp mtDNA was found to be more frequent among patients with peptic ulcer disease [52.7%] compared to the controls [15.3%]. A significant association was found between the deletion with peptic ulcer disease
Conclusion: Deletion of 4977 bp in mitochondrial DNA is associated with peptic ulcer disease
ABSTRACT
Colorectal cancer is the second cause of mortality in developed countries. The p53 gene and some of it's polymorphisms are among the causes for cancer development. The purpose of this study is to evaluate the relationship between the p53 codon 72 polymorphism in colorectal adenocarcinoma specimens compared with controls. We performed a case-control study among 112 patients with colorectal carcinoma and 112 controls in Rasht, Iran. Different genotypes of codon 72 were determined by allele-specific polymerase chain reaction [PCR]. The frequency of the Arg/Arg genotype of the p53 codon 72 polymorphism was 35.7%, for Arg/Pro it was 50.9%, and for Pro/Pro it was 13.4% in colorectal cancer patients. Among controls, the Arg/Arg genotype was seen 37.5%, Arg/Pro was noted in 50%, and Pro/Pro was seen in 12.5% [p = 0.95]. When tumor location was taken into consideration, 68.8% of the Arg/Arg carrier genotypes were associated with an increased incidence of left colon cancer. There was a significant statistical relationship between expression of the Arg allele in colorectal cancer samples and metastases [p=0.003]. The majority of colorectal cancer patients with p53 Arg homozygosity had left-sided colon cancer. There was also a relation between p53 Arg homozygosity, lymph node involvement, and metastases. Thus, we have suggested that the correlation between this polymorphism, tumor risk, and metastasis should be studied to determine its effectiveness as a diagnostic factor