ABSTRACT
Objective: To apply various hematological indices for the differential diagnosis of Beta-Thalassemia trait [Beta-TT] and iron deficiency anemia [IDA]
Methodology: This retrospective descriptive study was conducted at Dar-ul-Sehat Hospital, Gulistan -e- Johar, Karachi. We retrospectively analyzed complete blood count [CBC] of 2480 patients, who came to the OPD for various problems during the year 2014 from January to December so as to identify hypochromic microcytic patients. Mentzer's index [MI], Shine and Lal index [S and L index] and Ehsani's formula were applied on the CBC report of identified microcytic hypochromic patients
Results: It was found that among a total of 2840 patients, 385 [13.55%] patients were suffering from hypochromic microcytic anemia identified on their CBC report. These included 44 males [6.74%], 300 females [33.33%] and 41 [14.48%] children. Application of Mentzer's index [MI], Shine and Lal index [S and L index] and Ehsani's formula screened the hypochromic microcytic patients into patients suffering from ß-Thalassemia trait and Iron deficiency Anemia
Conclusion: Application of hematological indices can be taken as the most useful method fordifferentiating Beta-TT from IDA by simply considering CBC report
ABSTRACT
To determine the frequency of raised serum ferritin level in patients with metabolic syndrome. Case series study. Liaquat University Hospital Hyderabad. All the patients with metabolic syndrome for >/= 01 year duration, >/=20 years of age and of either sex were recruited and entered in the study. The metabolic syndrome was detected according to the NCEP-ATP III protocol. After confirmation of metabolic syndrome, the 2cc venous blood sample was taken in a sterilize 5cc disposable syringe, labeled it and sent to laboratory for evaluation of serum ferritin levels. The data was analyzed in SPSS 16 and the frequency and percentage was calculated. Total one hundred patients with metabolic syndrome were evaluated for serum ferritin level during the study period. The mean +/- SD for age of patients with metabolic syndrome was 52.73 +/- 7.83 while the mean age +/- SD of patient with raised ferritin level was 50.23 +/- 8.21. The majority patients were 30-49 age group with female predominance [p<0.01] and exist four component of metabolic syndrome. The ferritin was raised in 60 patients with female predominance [p<0.04]. The mean +/- SD of raised ferritin level in male and female population was 350.10 +/- 19.38 and 270.34 +/- 34.39 [p<0.01]. The raised serum ferritin level was identified [60%] with female predominance in patients of metabolic syndrome
Subject(s)
Humans , Female , Male , Middle Aged , Adult , Ferritins/blood , IronABSTRACT
To determine the cardiac manifestations in patients with pulmonary tuberculosis. Case series study. Jan 2014 to June 2014. Liaquat University Hospital Hyderabad. All the patients presented with shortness of breath, cough with sputum, fever, haemoptysis, anorexia and weight loss were recruited and then the subjects with early morning sputum positive for acid fast bacilli and radiological lesions suggestive of pulmonary tuberculosis, of ?20 years of age and either gender were enrolled and entered in the study. The data was analyzed in SPSS 16 and the frequency and percentage was calculated. Total one hundred patients with pulmonary tuberculosis were evaluated for cardiac manifestation during six months study period. The mean +/- SD for age of patients with pulmonary tuberculosis was 45.21 +/- 8.95. The mean age +/- SD of patient with cardiac manifestations was 48.95 +/- 5.53. The majority of the subjects were in 30-49 years age group with male predominance [p<0.01]. The electrocardiographic findings were observed in 72/100 [72%] patients whereas echocardiographic changes were observed in 50/72 [69.4%] patients with pulmonary tuberculosis. The common ECG findings identified were tachycardia 30.5%, P-pulmonale 12.5% and PR prolonged in 9.7% patients with tuberculosis whereas the common echocardiographic findings noticed were pericardial effusion 42% and multiple abnormalities in 24% subjects with tuberculosis. The sinus tachycardia, cor-pulmonale and low voltage QRS complexes were predominant abnormalities on electrocardiograph whereas pericardial effusion was the common abnormality on echocardiography in patients with pulmonary tuberculosis
ABSTRACT
To determine the frequency of raised C-reactive protein [CRP] in patients with type 2 diabetes mellitus. This cross sectional descriptive study of six months study was conducted at Liaquat University Hospital Hyderabad from March 2013 to August 2013. All diabetic patients of >/=35 years age of either gender for >01 year duration visited at OPD were evaluated for C-reactive protein and their glycemic status by hemoglobin A1c. The data was analyzed in SPSS and the frequency and percentage was calculated. During six month study period, total 100 diabetic patients were evaluated for C-reactive protein. Majority of patients were from urban areas 75/100 [75%]. The mean +/-SD for age of patients with diabetes mellitus was 51.63+/-7.82. The mean age +/-SD of patient with raised CRP was 53+/-7.21. The mean +/-SD for HbA1c in patients with raised CRP is 9.55+/-1.73. The mean random blood sugar level in patients with raised CRP was 247.42 +/- 6.62. The majority of subjects from 50-69 years of age group with female predominance [p = 0.01] while the CRP was raised in 70[70%] patients in relation to age [p=0.02] and gender [p=0.01] respectively. Both HbA1c and CRP was raised in 64.9% [p = 0.04] in patients with type 2 diabetes mellitus. The mean +/-SD of CRP was 5.8+/-1.21 while for male and female individuals with raised CRP was 3.52+/-1.22 and 5.7+/-1.63 respectively. The raised CRP was observed in patients with type 2 diabetes mellitus
Subject(s)
Humans , Female , Male , Adult , Middle Aged , Aged , Aged, 80 and over , C-Reactive Protein , Cross-Sectional Studies , Glycated Hemoglobin , Blood Glucose , Glycemic IndexABSTRACT
People usually give least importance to their oral hyiegene as a consequence they are suffering from so many disease due to oral biofilm that form over the teeth. Also when patient is going for dental procedures they were exposed to water from the dental lines of this unit. Pseudomonas aeroginosa were the main culprit present in these water line and form biofilm there as well as in host causing infection. Biofilm forming property confers virulence characteristics thus making such bacteria highly resistant to commonly used antibiotics. We analyzed the antibiofilm activity of natural occurring substances like dandasa [juglans regia] and amaltas [Cassia fistula]. Experimental study. All clinical isolates were identified at BUMDC and PNS microbiology lab [Karachi] by standard biochemical methods. This study was conducted between Nov 2012 to Feb 2013. 40 streptococcus mutan and 32 pseudomonas aerogenosa samples were taken from oral biofilm and contaminated denal lines water respectively and characterized using conventional biochemical, cultural and molecular methods. Biofilm forming activity of these isolates were checked. Then I mixed the dandasa and amaltas and observe the antibiofilm forming activity. Both dandasa and amaltas in a cone, of 12.5 mg/ml and 3.2 mg/ml respectively show good antibiofilm forming activity against pseudomonas aerogenosa and in a cone 0.8 mg/ml and 12.5 mg/ml respectively against streptococcus mutan. But combinations of dandasa with amaltas were found to be not more effective in inhibiting biofilm formation suggesting a indifferent activity with antiadhesive index of 1.0 and 0.75 against streptococcus mutans, and pseudomonas aerogenosa respectively. Streptococcus mutans in oral biofilm and pseudomonas in dental water lines exhibited biofilm formation which is the cause for antibiotic resistance and providing shelter to other organism. Amaltas and Dandasa provide a good antibiofilm activity individually
ABSTRACT
The present study was designed to find the prevalence of gestational Diabetes mellitus [GDM] in patients attending Diabetic Clinic of Sir Syed Hospital from June 2005 to December 2005 with gestational age between 24[th] to 28[th] weeks with or without risk factors. Fifty gm glucose challenge test was used for screening with cut off value of blood glucose > 130 mg/dl [7.2 mmol/L]. Out of 405 women seventy-two women screened positive and 100 gm oral glucose tolerance test was offered and seventeen women were diagnosed as gestational diabetes mellitus. Eight women were classified as impaired glucose tolerance while the gestational diabetes mellitus is more prevalent in high risk groups. The whole method was applied on pregnant female, irrespective of the last meal taken. Age between 20-40 yrs, and avoiding severely ill, and known diabetes