ABSTRACT
The present paper investigates antioxidant, antimicrobial and photochemical screening different extracts of Fagonia olivieri. Analysis of the data indicated that the subject plant contained a good amount of flavonoids, tannins, saponins, terpenoids and steroids. Maximum concentrations of phenolic compounds was found in methanol fraction [29.0+/-6.12 mg GAE/g] while minimum [22.10+/-6.31mg GAE/g] in methylated spirit fraction. Similarly, ethanol fraction contained higher concentration of flavonoid content [135.4+/-7.63mg Quercetin/g] followed by methanol fraction [138.4+/-2.96 mg Quercetin/g]. Analysis of the data revealed that maximum antioxidant activity was recorded in mthylated spirit fraction [IC[50]= 10.69+/-1.66] followed by methanol fraction [IC[50]= 9.10+/-0.76] while no activity was noted in hexane fraction. The data indicated good antibacterial and antifungal activity against S. typhi, S. aureus, P. aeruginosa and A. flavus
ABSTRACT
The present study reveals antimicrobial potentials and phytochemical analysis of A. javanica and L. ustitatissimum. Phytochemical analysis indicated that the tested plants contained a substantial amount of flavonoids, terpenoids and steroids while saponins and tannins were absent in L. ustitatissimum, however, tannins were present in A. javanica. L. ustitatissimum contained maximum total phenolic content of 166.36mg/g in methylated spirit fraction while its ethyl acetate fraction contained highest quantity of flavonoids 27.6mg/g in case of Aerva javanica. Antimicrobial potentials of the subject plants revealed that L. ustitatissimum had maximum antibacterial activity [MIC=4.33microg/ml] while A. javanica was most effective against fungal strains [MIC=2.66 microg/ml]
ABSTRACT
The present study evaluates the phytochemical screenings, antioxidant activity and antimicrobial assay of Apium graveolens L. The phytochemical screening showed the presence of flavonoids, tannins, saponins and steroids in Apium graveolens while terpenoids was absent. The total phenolic content was slightly higher in methanolic fraction [63.46 +/- 12.00mg GAE/g] followed by ethanol [36.60 +/- 12.28 mg GAE/g] and hexane fractions [34.86 +/- 6.96mg GAE/g]. The flavonoid content was high in methanolic extract [56.95 +/- 7.14mg Quorcetin/g] and low level of the content was found in methylated spirit extract [29.2 +/- 3.15mg Quercitin/g]. Antioxidant activity assayed by FRAP was higher in methanolic fraction [12.48 +/- 1.06 mmole of FeSO[4] equivalent/litre of extract] compared with other extracts. Likewise, good antimicrobial activity was measured by crude ethanol fraction against S. aureus [MIC=0.12 +/- 0.03microg/ml] and S. typhi [MIC= 0.5 +/- 0.2microg/ml]. Results also that ethanolic fraction was effective against A. flavus [MIC= 05 +/- 1.0microg/ml]
Subject(s)
Phytochemicals , In Vitro Techniques , Antioxidants , Plant Extracts , Plants, Medicinal , Anti-Infective AgentsABSTRACT
The Grewia asiatica [commonly known as Phalsa or Fasla] is a shrub or small tree found in southern Asia. It produces purple to black color fruit when ripe. In folk medicine the edible Grewia asiatica fruit is used in a number of pathological conditions. The current study described the effects of Grewia asiatica fruit on glycemic index [GI] and phagocytosis in healthy non-diabetic human subjects. The results showed that Grewia asiatica fruit has low GI value of 5.34 with modest hypoglycemic activity. Luminol-enhanced chemiluminescence assay was carried out to determine the production of reactive oxygen species [ROS] in the oxidative burst activity of whole blood. ROS production was found to be significantly affected, having the 78.3, 58.6 and 30.8% when the subjects were fed with D-glucose, mixture of D-glucose and Grewia asiatica fruit and Grewia asiatica fruit alone respectively as compared to the control. The aqueous, methanolic and butanolic extracts of Grewia asiatica fruits were found to produce a stimulatory effect on ROS production however; the chloroform, hexane and ethanol-acetate extracted exerted significant inhibitory effect. These results demonstrated that Grewia asiatica fruit has desirable effects on blood glucose metabolism manifested as low glycemic response and modulation of ROS production