ABSTRACT
Ovarian cancer remains the leading cause of death from gynecologic malignancy in western countries. This cancer results from a succession of genetic alterations involving oncogenes and tumor suppressor genes, which have a critical role in normal cell growth regulations. Forty-nine patients were studied for p53 mutations detection using combination of PCR amplification and DGGE which allows comprehensive mutation scanning for exons [5-8], mutation were confirmed by DNA gene sequencing. From 49 cases 5 cases showed mutation [10.2%] 3 mutations were detected in exon 6, one in exon 8, one in exon 5 [3] and all were point mutations. Of total mutations detected four were serous adenocarcinoma [4/24] [16.7%] and one was undifferentiated carcinoma. Detection of gene mutations by DGGE has proved to be a very accurate strategy