Production of growth hormones by feankia strain HFPCg14 in defined culture medium

The excretion of growth hormones in defined culture medium by Frankia strain HFPCg14 was examined. The amounts of these compounds in extracts fractionated by paper partition chromatography were estimated by bioassay. Three auxin-like compounds, two cytokinin-like compounds and three gibberellin-like compounds were detected in culture media. Indole-3acetic acid [IAA] and gibberellin [GA3] was the main active compounds released during the logarithmic phase of growth by Frankia strain HFPCgl4

Performance of two casuarina species inoculated with pure culture of frankia strain under field conditions

A field trial was conducted for two different Casuarina species, C. cunninghamiana and C. Hybrid [C. Glauca X C. Cunninghamiana] to evaluate their performance in response to inoculation with Frankia strain HFPCgI4. Biomass productivity [expressed as plant dry weight], plant volume, N concentration [%], total nitrogen content and nodule dry weight were measured in inoculated and uninoculated plants. Inoculation increased markedly the performance of the tested Casuarina species. Meanwhile, there were differences between the inoculated Casuarina species in the measured parameters. Differences were also observed in the proportion of N derived from nitrogen fixation and in the total N content of these species. The amount of fixed nitrogen was highly correlated with the total nitrogen content. However, the correlation between the amount of fixed N2 and plant volume, plant dry weight and nodule dry weight did not always show positive correlation. An increment in the measured parameters was observed when low level of phosphorus [2.6 mg/kg soil] was applied as a fertilizer. From the data obtained, inoculation proved to be the best for higher ability of nitrogen fixation as well as a good performance under field conditions. Casuarina hybrid showed a higher ability for nitrogen fixation