potential role of some antioxidants from carotenoids and vitamins on biological and biochemical parameters of hyperlipidemic hamsters

This study was conducted to elucidate the effect of some antioxidants [carotenoids: lycopene and beta-carotene and vitamins: E and C] supplementation on some biological and biochemical parameters of male adult hyperlipidemic hamsters. Lipid profile, lipid peroxidation as MDA, blood and liver GSH, antioxidant enzymes [GPx and GR] and liver function enzymes [ALT and AST] were determined. Hyperlipidemic diet resulted in significant elevation in serum TC, LDL-C, TG, liver MDA and liver function enzymes ALT and AST, with concomitant reduction in serum HDL-C, blood GSH and liver GPx and GR enzymes activity. Supplementation by the Lycopene and vitamin C caused significant reduction in serum TC, LDL-C and TG with significant elevation in serum HDL-C. beta-carotene induced significant reduction only on TC and LDL-c vitamin E showed no obvious effect on lipid profile with highly suppressive effect on lipid peroxidation all individual antioxidants resulted in significant elevation in blood GSH, liver GPx and GR enzymes with concomitant significant reduction in liver MDA and serum ALT and AST

Preparation of 99mTc-carnosine and 99mTcO-[V]-DMSA complexes, biological distribution, and estimation of their gene anti- polymorphisms induced by gamma-irradiation

Two chelating agents [Carnosine and DMSA] were used to study their labeling conditions with technetium-99m followed by biological distribution investigation. Molecular studies were done via PCR/RFLP analysis of angiotensin II subtype II receptor gene for monitoring their antioxidant activity through free iron chelation leading to inhibition of Fenton reaction. Carnosine was labeled by mixing 4 mg with 30 mg glucose and 25 micro g SnCl2.2H2O, followed by pertechnetate and stand at room temperature for 60 minutes. Minor modification was done to prepare 99mTc[V]-DMSA tracer in one step, by adding pertechnetate solution to the lyophilized kit contains 1mg DMSA, 0.1 mg SnCl2.2H2O, and 30 mg glucose at pH 9. The biodistribution of the two tracers in normal and tumor-induced mice. The molecular investigation of the anti-oxidant activity of both carnosine and DMSA in 6 Gy gamma-irradiated rats using the anti-inflammatory angiotensin II subtype II receptor gene [AT2RG] as indicator. Carnosine and DMSA were labeled with Technetium-99m yielding 85% and 97%, respectively the ability of both tracers to localize in tumor sites but the priority to the 99mTc [V]-DMSA. Molecular studies showed strong antioxidant activity of carnosine but not enough to block radiation induced oxidative stress and Moderate antioxidant activity of DMSA was achieved by chelating free iron and iron released through oxidative stress. Maximum protection was achieved through the dual action of both DMSA and carnosine. moderate and high labeling yield were achieved for both 99mTc[V]DMSA and 99mTc-canosine respectively with higher selectivity of the former to tumor sites and maximum protection were achieved by the dual action of both chelating agents