ABSTRACT
Background: Preeclampsia [PE] is a complex and serious multi-system disorder of pregnancy with a worldwide incidence of 5-7% and contributes significantly to maternal and perinatal morbidity and mortality. Normal pregnancy is associated with a local hypercoagulable state that becomes more profound in PE. Histidine-rich glycoprotein [HRG] is a protein interacting with angiogenesis, coagulation, and inflammatory responses, processes known to be altered in preeclamptic pregnancies
Aim of the work: Is to analyze changes in the circulating levels of plasma factor VII [Plasma F VII] and HRG in women developing PE and to evaluate them as markers for early diagnosis of PE
Subjects and Methods: This study was carried out on 80 pregnant women after 20 weeks of gestation. The subjects were divided into: Group I [G1]: 40 cases with preeclampsia; Group II [G2]: 40 normal pregnant women who were matched for age and gestational period. Plasma FVII and HRG were measured by ELISA
Results: Plasma FVII levels were significantly higher in G1 [206.22 +/- 46.25 ng/ml] as compared to G2 [97.46 +/- 21.95 ng/ml] [p<0.001]. Significant positive correlation of plasma FVII with fibrinogen and significant negative correlation with platelet count and HRG were detected. At cut off 125.75 ng/ml plasma FVII levels shows high sensitivity and specificity [95%]. HRG levels were significantly lower in G1 [37.1 +/- 7.52 pg/ml] as compared with G2 [79.87+/-24.15 pg/ml] [p<0.001]. HRG shows significant positive correlation with platelet count and significant negative correlation with each of fibrinogen and plasma FVII. At cut off 46.45 pg/ml. HRG shows high sensitivity [92.5%] and specificity [87.5%]
Conclusion: Plasma FVII and HRG can be used as early marker for detection of PE