ABSTRACT
Two polymerase chain reaction (PCR) protocols showed low sensitivity (36% and 53% for TB AMPLICOR and MPB64 nested PCR, respectively), when compared with classic microbiological methods (73% and 54% for Ziehl-Neelsen staining and culture, respectively), in the diagnosis of tuberculous meningitis in 91 patients in southeastern Brazil. Only three PCR-positive, microbiologically negative patients were found. Analysis of sequential cerebrospinal fluid samples by nested PCR detected Mycobacterium tuberculosis DNA up to 29 days after the introduction of antituberculosis chemotherapy.
Exames coproparasitológicos realizados em 191 crianças de creches e em 434 alunos da primeira à quarta série das áreas urbana e rural da rede municipal de Rolândia, PR, evidenciaram enteroparasitas em prevalência de 15,2% nas creches e de 52,5% entre os escolares. Fatores de risco são discutidos.
Subject(s)
Humans , Polymerase Chain Reaction , Tuberculosis, Meningeal/diagnosis , Brazil , Prospective Studies , Sensitivity and SpecificityABSTRACT
The activity of important glycolytic enzymes (hexokinase, phosphofructokinase, aldolase, phosphohexoseisomerase, pyruvate kinase and lactate dehydrogenase) and glutaminolytic enzymes (phosphate-dependent glutaminase) was determined in the thymus and mesenteric lymph nodes of wistar rats submited to protein malnutrition (6 percent protein in the diet rather than 20 percent) from conception to 12 weeks after birth. The wet weight (g) of the thymus and mesenteric lymph nodes decreased due to protein malnutrition by 87 percent (from 0.30 + 0.05 to 0.04 + 0.01) and 75 percent (0.40 + 0.04 to 0.10 + 0.02), respectively. The protein content was reduced only in the thymus from 102.3 + 4.4 (control rats) to 72.6 + 6.6 (malnourished rats). The glycolytic enzymes were not affected by protein malnutrition, but the glutaminase activity of the thymus and lymph nodes was reduced by halfing in protein-malnourished rats as compared to controls. This fact may lead to a decrease in the cellularity of the organ and thus in its size, weight and protein content.
Subject(s)
Rats , Male , Animals , Dietary Proteins/metabolism , Fructose-Bisphosphate Aldolase/metabolism , Glucose-6-Phosphate Isomerase/metabolism , Glucose/metabolism , Glutaminase/metabolism , Glutamine/metabolism , Glycolysis/physiology , Hexokinase/metabolism , L-Lactate Dehydrogenase/metabolism , Lymph Nodes/enzymology , Phosphofructokinase-1/metabolism , /metabolism , Protein-Energy Malnutrition/enzymology , Pyruvate Kinase/metabolism , Thymus Gland/enzymology , Rats, WistarABSTRACT
Em inquérito realizado na zona urbana de Cáceres (Mato Grosso, Brasil) foram estudados 485 homens e 766 mulheres maiores de 10 anos de idade. Neles a prevalência de Hipertensäo arterial foi de 12,2%. Os critérios utilizados foram os propostos pela OMS. As mulheres apresentaram prevalência maior que a dos homens, e em ambos os sexos a prevalência aumenta com a idade. Dentre os hipertensos poucos titinham consciência de seu estado e raros estavam em tratamento