ABSTRACT
Background: Zincovit (ZVT) syrup is a combined formulation of vitamins, minerals and lysine. The present study was aimed to investigate the in vivo antioxidant potential of ZVT syrup in carbon tetrachloride (CCl4) intoxicated Wistar rats. Methods: A total of 36 Wistar rats were divided into six groups of six rats each. Antioxidant potential of ZVT syrup at the dose of 15 mg/kg/day, 30 mg/kg/day and 60 mg/kg/day was evaluated in CCl4 intoxicated rats. The extent of CCl4 induced oxidative stress was studied by estimating malondialdehyde (MDA), glutathione-Stransferase (GST) and catalase (CAT). Results: Oral treatment with ZVT syrup, especially at the dose of 30 mg/kg/day and 60 mg/kg/day reversed CCl4 - induced alterations in MDA (p<0.05), GST (p<0.01) and CAT (p<0.01) compared to CCl4 intoxicated control (untreated) animals. Conclusion: The present findings revealed that ZVT syrup may be useful in oxidative stress associated tissue damage as a nutritional food supplement.
ABSTRACT
Background: Zincoderm G cream is a fixed dose combination of clobetasol, gentamicin and zinc. Studies have been carried out for efficacy of each component such as clobetasol and gentamicin in bacterial infections, but as fixed dose combination including zinc has not been reported yet. Hence, a study was planned to assess the efficacy of Zincoderm G cream in experimental wound infection by Pseudomonas aerugionosa. Methods: In the experiment a total of 18 Sprague-Dawley rats (male, pathogen free, 6-8 weeks old) were used. The rats were divided into three groups of six rats each. 25-30 mg of test drugs (Zincoderm G cream with or without zinc) was applied on Pseudomonas aeruginosa infected burn wound affected area of back of rats for 2 weeks. Bacterial infection was assessed by quantification of bacteria. Results: There was 80% mortality observed in P. aeruginosa infected toxic control (cream base) group. Whereas, only 40% mortality was seen in both Zincoderm G cream with/without zinc groups, which were inoculated with P. aeruginosa. Bacterial concentration (Number of colony forming unit/ml wound fluid) was significantly decreased (p<0.001) in P. aeruginosa infected rats treated with Zincoderm G cream with zinc when compared with P. aeruginosa infected control (untreated) rats. Conclusions: We found that Zincoderm G cream with zinc exhibited distinct killing profiles against P. aeruginosa.