Niveles séricos de la isoenzima creatina quinasa-MB y lactato deshidrogenasa como indicadores de daño miocárdico en perros con enfermedad valvular degenerativa / Serum levels of the isoenzyme creatine kinase-MB and lactate dehydrogenase as indicators of myocardial damage in dogs with degenerative valve disease

RESUMEN Se analizaron los niveles séricos de creatina quinasa-MB (CK-MB) y lactato deshidrogenasa (LDH) en 10 perros diagnosticados con enfermedad valvular degenerativa y en seis perros clinicamente sanos, con el objetivo de evaluar si sus niveles séricos indican daño miocárdico. Las muestras de suero se analizaron mediante el método UV. Se utilizó la prueba de diferenciación de medias para determinar diferencias entre medias, y la prueba de correlación de Pearson para determinar si existe correlación entre los niveles séricos de ambas enzimas. Los valores de CK-MB y de LDH fueron significativamente diferentes entre los dos grupos de pacientes. Los niveles de CK-MB y LDH tuvieron correlación positiva, pero no significativa.

ABSTRACT Serum levels of creatine kinase-MB (CK-MB) and lactate dehydrogenase (LDH) were analyzed in 10 dogs diagnosed with degenerative valvular disease and in six clinically healthy dogs with the objective of evaluating whether their serum levels indicate myocardial damage. Serum samples were analyzed by UV method. The mean differentiation test was used to determine differences between means and the Pearson correlation test was performed to determine if there was a correlation between the serum levels of both enzymes. The CK-MB and LDH values were significantly different between the two groups of patients. The levels of CK-MB and LDH had a positive but not significant correlation.

Emergence of multidrug-resistant bacteria isolated from surgical site infection in dogs and cats / [Emergência de bactérias multirresistentes isoladas da infecção no sítio cirúrgico em cães e gatos]

Surgical site infections (SSIs) and antimicrobial resistance among pathogens causing SSI are a growing concern in veterinary hospitals. One major reason, the widespread use of antimicrobials, has led to increased incidence of SSIs. This study identified bacteria and resistance profiles to antimicrobials in the SSI cases diagnosed at the Surgical Clinic of Small Animals in the Veterinary Hospital, Federal University of Viçosa, Brazil. The main genus identified was Staphylococcus, followed by Escherichia, Enterococcus, Bacillus, Shigella, Citrobacter, Proteus, Morganella, Serratia, Enterobacter, Pseudomonas and Klebsiella were also found, but in small number. The results indicated the predominance of Gram-negative bacteria among the collected samples. Most of isolates identified were resistant to more than one of the following antimicrobials: ampicillin, tetracycline, enrofloxacin, amoxicillin/clavulanic acid and cephalotin. Of the 17 Staphylococcus sp. isolates, two (11.8%) were methicillin-resistant Staphylococcus aureus (MRSA) and 11 (64.7%) of them were methicillin-resistant Staphylococcus pseudintermedius (MRSP). There were bacterial genera identified with resistance to all tested antimicrobials in different proportions. This should alert veterinary hospitals to the emergence of multidrug-resistant bacteria and to the requirement for the revision of surgical protocols with regard to antimicrobial prophylaxis and therapy.(AU)

As infecções em sítio cirúrgico (ISCs) e a resistência bacteriana entre os patógenos relacionados constituem uma preocupação crescente nos hospitais veterinários. O aumento na incidência de ISCs possui forte relação com o uso amplo e disseminado de antibióticos. O presente estudo identificou bactérias e perfis de resistência a antibióticos nos casos de ISCs diagnosticados na Clínica Cirúrgica de Pequenos Animais do Hospital Veterinário da Universidade Federal de Viçosa, Brasil. O principal gênero identificado foi Staphylococcus, seguido pelos gêneros Escherichia, Enterococcus, Bacillus, Shigella, Citrobacter, Proteus, Morganella, Serratia, Enterobacter, Pseudomonas e Klebsiella, porém, em menor quantidade. Os resultados demonstraram a predominância de bactérias Gram-negativas entre as amostras coletadas. A maioria dos isolados identificados eram resistentes a um ou a mais de um dos seguintes antibióticos: ampicilina, tetraciclina, enrofloxacina, amoxicilina/ácido clavulânico e cefalotina. Entre os 17 isolados de Staphylococcus sp., dois (11,8%) eram Staphylococcus aureus resistentes à meticilina (SARM) e 11 (64,7%) eram Staphylococcus pseudintermedius resistentes à meticilina (SPRM). Houve identificação de gêneros bacterianos com diferentes proporções de resistência para todos os antibióticos avaliados. Esses achados devem alertar os hospitais veterinários para a emergência de bactérias multirresistentes e para a necessidade de revisar a profilaxia e a terapia antimicrobiana referente aos protocolos cirúrgicos.(AU)

Modelo de indução de osteoporose em coelhas - avaliação radiográfica / Rabbit osteoporosis induction model - radiographic evaluation

In order to establish a bone loss induction model in rabbits to study osteoporosis, 18 rabbits underwent ovariectomy and received methylprednisolone 1mg/kg intramuscularly on alternate days for two weeks. Immediately before ovariectomy up to 42 days after methylprednisolone administration, radiographs of the right olecranon were taken in the mediolateral position to evaluate the radiographic optical density. Before the induction of osteoporosis, rabbits presented mean values of radiographic density of 2.305mm Al and at 42 days of 1.575mm Al. The values obtained were submitted to ANOVA for repeated measures that revealed a significant drop (P< 0.001) in the density over time, proving that the induction was able to trigger bone loss of these animals. With this, it can be affirmed that the adopted protocol was enough to provoke a significant bone loss, characterizing a valid model for the study of new treatments for osteoporosis.(AU)

Timo cervical aberrante: masa cervical pediátrica inusual, revisión bibliográfica y reporte de caso / Aberrant cervical thymus: unusual type of pediatric cervical mass, bibliographic review and case report

Las masas cervicales representan un desafío habitual en pediatría, que en su mayoría son de etiología benigna. Una de las causas menos conocidas es el timo cervical aberrante, una entidad clínica resultante de anormalidades en el descenso del timo a lo largo de su ruta habitual (tracto timo-faríngeo). Este es un cuadro frecuentemente mal diagnosticado, del que se desconoce su incidencia real. Actualmente, su incidencia relativa parece ir en aumento en conjunto con la disponibilidad de la ecotomografía. En Chile no hay registro epidemiológico de este cuadro ni existen reportes de caso documentados. En este artículo se presenta una revisión bibliográfica sobre masa cervical pediátrica y un reporte de caso de un paciente chileno.

Cervical masses constitute a frequent challenge in pediatric care, mostly which are of benign nature. One of the lesser known causes is the aberrant cervical thymus, which results from embryological abnormalities during the thymus descent through its normal route (thymo-pharyngeal duct). This is a frequently misdiagnosed syndrome, for which its real incidence remains unknown. Nowadays, its relative incidence is on the rise along with ultrasound availability. There is no epidemiological record or documented case reports of this entity in Chile. In this article we present a bibliographic revision on cervical mass and a case report from a Chilean patient.

Características antropométricas y somatotipo en seleccionados chilenos de remo / Anthropometric characteristics and somatotype in elite Chilean rowers

El objetivo del estudio fue describir las características antropométricas y somatotipo de los seleccionados chilenos de remo. Se evaluaron a 21 deportistas (6 mujeres y 15 hombres) con edades promedio de 18,66 ± 2,58 años para las mujeres y de 22,06 ± 4,71 años para los hombres. El estudio se realizó previo al clasificatorio Panamericano Lima 2019, al momento de las evaluaciones, los deportistas se encontraban concentrados en el centro de entrenamiento olímpico (CEO) ubicado en Curauma en la región de Valparaíso en Chile. Las evaluaciones antropométricas se realizaron en base al perfil restringido de 25 variables propuesto por Ross & Kerr (1991), que permitió fraccionar la masa corporal en 5 componentes (tejidos: piel, residual, óseo, adiposo y muscular). Se midieron también la envergadura, la altura ilioespinal y los pliegues del bíceps y supracrestídeo, todas las medidas se realizaron en base al protocolo de marcaje y evaluación propuesto por ISAK. Se uso el método de Carter y Heath para calcular el somatotipo, describiendo los componentes de endomorfía, mesomorfía y ectomorfía respectivamente. Los resultados mostraron un porcentaje de grasa en hombres de 19,34 ± 1,59 % y en mujeres de 27,08 ± 3,6 % (p=0,003), la masa muscular de hombres fue de 52,69 ± 1,78 % y en mujeres de 45,68 ± 4,19 % (p=0,003), se apreció un somatotipo mesomorfo balanceado tanto en hombres (1,7-5,3-2,2) como mujeres (2,5-3,7-2,7). Se concluye que los seleccionados chilenos de remo presentan un alto desarrollo de masa muscular y un bajo porcentaje de masa grasa, además de un somatotipo mesomorfo balanceado.

The aim of the study was to describe the anthropometric characteristics and somatotype of the Chilean selected rowers. Twenty one 21 athletes (6 women and 15 men) with an average age of 18.66 ± 2.58 years for women and 22.06 ± 4.71 years for men were evaluated. The study was conducted prior to the Pan American qualification in Lima 2019. At the time of the evaluations, the athletes were concentrated in the Olympic Training Center (CEO) located in Curauma in the Valparaíso region of Chile. The anthropometric evaluations were carried out based on the restricted profile of 25 variables proposed by Kerr and Ross that allowed the fractionation of the body mass into 5 components (tissue: skin, residual, bone, adipose and muscle). The wingspan, the iliospinal height and the folds of the biceps and supracrestide were also measured; all measurements were based on the protocol of marking and evaluation proposed by ISAK. Carter and Heath method was used to calculate the somatotype, describing the components of endomorphy, mesomorphy and ectomorphy respectively. The results showed a percentage of fat in men of 19.34 ± 1.59 % and in women of 27.08 ± 3.6 % (p = 0.003), the muscle mass of men was 52.69 ± 1.78 % and in women of 45.68 ± 4.19 % (p = 0.003), a balanced mesomorphic somatotype was observed in both men (1.7-5.3-2.2) and women (2.5-3, 7-2.7). It is concluded that the Chilean rowing selected have a high development of muscle mass and a low percentage of fat mass, in addition to a balanced mesomorphic somatotype.

Estimulación Magnética Trascraneal "Theta-Burst Intermitente" en un paciente con Trastorno del Espectro Autista: Reporte de un caso / Intermittent Theta-Burst Transcranial Magnetic Stimulation in a Patient with Autism Spectrum Disorder: A Case Report

Resumen Introducción: La estimulación magnética transcraneal (EMT) es una opción potencial de tratamiento para la sintomatología de trastorno del espectro autista. Objetivos: Determinar la eficacia de la EMT en modalidad theta-burst intermitente (ETBi) sobre la corteza pre frontal dorso lateral izquierda (CDLPF) en el manejo de comportamientos patológicos asociados con TEA. Pacientes y Métodos: Paciente masculino de 10 años de edad diagnosticado con TEA con (ADOS) Autistic Diagnostic Observation Schedule, presenta una historia familiar de una hermana menor con TEA. Tanto el estudio de Resonancia Magnética encéfalo (IRM) y IRM funcional (IRMf) con paradigma emocionales se efectuaron antes del tratamiento de ETBi, inmediatamente después de tratamiento ETBi, y después de 6 meses de tratamiento con ETBi. Resultados: La prueba de ATEC reporto mejoría en las escalas de comunicación, cognición, social y comportamiento, 83%,81%,72% y 52% respectivamente. La puntuación total mejoro un 66% inmediatamente después de tratamiento y 55% en un periodo de 6 meses. La IRMf revelo la activación de la corteza frontal, parietal y occipital antes del tratamiento con ETBi y persistiendo la mejoría por 6 meses. Conclusión: ETBi una técnica de neuromodulación no invasiva bien tolerado que requiere un tiempo de administración menor que el tratamiento estándar.

Abstract Background: Transcranial magnetic stimulation (TMS) is a potential treatment option for autism spectrum disorder (ASD) symptomatology. Objectives: To determine the efficacy of the TMS intermittent theta-burst stimulation (iTBS) protocol over the left dorsolateral prefrontal cortex (DLPFC) in the management of pathological behaviors associated with ASD. Patient/Methods: A 10-year-old male diagnosed with ASD by the Autism Diagnostic Observation Schedule (ADOS) presented with a family history of ASD with a younger sister diagnosed with the same disorder. Both magnetic resonance imaging (MRI) and functional MRI (fMRI) scans for emotional paradigms were performed before the iTBS treatment, immediately after the iTBS treatment, and after 6 months of iTBS treatment. Results: The ATEC reports revealed improvement in communication, cognition, sociability, and behavior scales by 83%, 81%, 72%, and 52%, respectively. The overall score improved by 66% immediately after the treatment and by 55% lasting over a 6-month period. The fMRI revealed the activation of the frontal, parietal, and occipital cortex before iTBS treatment, and a better integration and activation of the frontal, temporal, and occipital cortex after iTBS treatment and persisted after 6 months. Conclusions: iTBS is a well-tolerated, non-invasive neuromodulation technique that requires relatively less administration than the standard treatment.

Composición corporal y somatotipo de rugbistas chilenos y su relación con la posición de juego / Body composition and somatotype of chilean rugby players and their relationship with the game position

RESUMEN: El objetivo del estudio fue describir las características morfoestructurales, por posición de juego, en rugbistas de la región de Valparaíso, Chile. La estatura, peso, índice de masa corporal (IMC) y otros 25 parámetros antropométricos fueron obtenidos desde 121 rugbistas competitivos (24,2 ± 4,9 años), siguiendo los estándares de medición de la Sociedad Internacional para el Avance de la Kinantropometría (ISAK). La composición corporal fue descrita en 5 componentes a través del método propuesto por Kerr, estos son masa muscular, adiposa, residual, ósea y piel. El uso del somatotipo descrito por Carter y Heath, permitió describir los componentes de endomorfía, mesomorfía y ectomorfía. Para comparar las características morfoestructurales de los rugbistas por posición de juego, estos fueron separados en forwards y backs. El peso corporal, la estatura sentado y de pie, el IMC y la sumatoria de 8 pliegues fueron mayores en los forwards que los backs (p<0,0001). En relación a las masas corporales, el tejido adiposo y muscular absoluto fueron mayores en los forwards que en los backs (P< 0,01), no así el relativo. El somatotipo evidenció mayor endomorfía y mesomorfía, y menor ectomorfía, en los forwards 4,8-6,9-0,7 (1,8-1,3-0,8) que los backs 3,7-6,1-1,2 (1,8-1,1-0,8) con P<0,01. En conclusión, la posición de juego en el rugby es determinante en la definición de características físicas básicas y morfológicas. El mayor peso corporal de los forwards respecto a los backs se encuentra influenciado por la presencia de más tejido muscular y adiposo. Esto último se relacionó con un mayor componente mesomórfico y endomórfico del somatotipo, y menor del ectomórfico, en los forwards. A pesar de estas diferencias ambos grupos se encuentran en una clasificación de endo-mesomorfo.

SUMMARY: The objective of the study was to describe the morpho-structural characteristics according to playing position, in rugby players from the region of Valparaíso, Chile. Height, weight, body mass index (BMI) and other 25 anthropometric parameters were obtained from 121 competitive rugby players (24.2 ± 4.9 years), following the recommendations of the International Society for the advancement of Kinanthropometry. The body composition was described in 5 components through the method proposed by Kerr, they are muscle, fat, residual, bone and skin. The somatotype described by Carter and Heath, allowed to describe the components of endomorphy, mesomorphy and ectomorphy. To compare the morphostructural characteristics of the rugby players by playing position, these were separated into forwards and backs. Body weight, sitting and standing height, BMI and S 8-fold were higher in the forwards than the backs (p <0.0001). In relation to body mass, adipose tissue and absolute muscle were higher in the forwards than in the backs (P <0.01), but not in the relative. The somatotype showed significant differences in the averages of the three components between forwards 4.8-6.9-0.7 (1.8-1.3-0.8) and backs 3.7-6.1-1, 2 (1.8-1.1-0.8) with P <0.01. In conclusion, the playing position in rugby is decisive in the definition of basic physical and morphological characteristics. A higher body weight of the forwards versus backs is influenced by a greater muscle and adipose tissues. Latter was related to a greater mesomorphic and endomorphic component of the somatotype, and lower ectomorphic in the forwards. Despite these differences, both groups are classified as endo-mesomorph.

Treatment of periodontal disease with guided tissue regeneration technique using a hydroxyapatite and polycaprolactone membrane / Tratamento da doença periodontal pela técnica da regeneração tecidual guiada utilizando uma membrana de hidroxiapatita e policaprolactona

The aim of this study was to evaluate the use of a malleable membrane composed of hydroxyapatite (60%) and polycaprolactone (40%) as treatment of periodontal disease experimentally induced in dogs. A bone defect of standardized dimensions was created between the roots of the third and fourth premolar of 12 dogs for periodontal disease induction. Six dogs had the defect covered by the membrane and six dogs received only standard treatment for periodontal disease, also applied to dogs in the treated group. The animals were clinically monitored during the experiment. Radiographs were taken after surgery and at 60 days after treatment initiation. Clinical attachment level was also assessed in those moments. On the 60th day, dental sample of all animals, containing tooth, defect and periodontal tissues, were harvested, fixed in formalin and analyzed by microtomography and histology. During the experimental period, the animals showed no pain and purulent discharge, however, there was dehiscence in 50% of animals and membrane exposure in five out of six animals in the treated group. Clinical attachment level showed no difference between groups. Radiographs showed radiopacity equal to the alveolar bone in both groups. The microtomography revealed that the control group had higher bone volume in the defect compared to the treated group; however, the furcation was not filled by new alveolar bone in any animal. Histological analysis revealed that junctional epithelium invasion was lighter in the control group. New bone was only observed in the apical edge of the defect in both groups. Although the composite is biocompatible and able to keep the space of the defect, it did not promote periodontal tissue regeneration within 60 days of observation.(AU)

O objetivo do presente trabalho foi avaliar a utilização de membrana moldável constituída por hidroxiapatita (60%) e policaprolactona (40%) como tratamento da doença periodontal, induzida experimentalmente em cães. Um defeito ósseo de dimensões padronizadas foi realizado entre as raízes do terceiro e do quarto pré-molares de 12 cães para indução da doença periodontal. Todos os cães receberam tratamento padrão para doença periodontal, e seis desses animais foram tratados também com a aplicação da membrana sobre o defeito. Os animais foram acompanhados clinicamente durante o experimento. Radiografias foram realizadas no pós-operatório e aos 60 dias após o início do tratamento. O nível clínico de inserção também foi avaliado nesses momentos. Aos 60 dias, a amostra dental de todos os animais contendo o dente, o defeito e os tecidos periodontais foi coletada, fixada em formol e analisada por microtomografia e histologia. Durante o período experimental, os animais não apresentaram dor e secreção purulenta, entretanto houve deiscência em 50% dos animais e exposição de membrana em cinco dos seis animais do grupo tratado. Nível clínico de inserção não apresentou diferença entre os grupos. As imagens radiográficas mostraram radiopacidade igual ao osso alveolar em ambos os grupos. A microtomografia revelou que o grupo controle apresentou maior volume ósseo no defeito em relação ao grupo tratado, no entanto, em todos os animais, a região de furca não foi preenchida por novo osso alveolar. A análise histológica revelou que a invasão por epitélio juncional foi mais discreta no grupo controle. Osso novo foi apenas observado na borda apical do defeito em ambos os grupos. Embora o compósito seja biocompatível e tenha sido capaz de manter o espaço do defeito, ele não promoveu a regeneração dos tecidos periodontais no período de 60 dias de observação.(AU)

Hydroxyapatite, polycaprolactone and alendronate composites for bone regeneration in rabbits' olecranon: histological features / Compósitos de hidroxiapatita, policaprolactona e alendronato na regeneração óssea em olécrano de coelhos: características histológicas

O presente trabalho teve como objetivo avaliar a biocompatibilidade e a osseointegração de compósitos de hidroxiapatita (HA), policaprolactona (PCL) e alendronato (ALN) em defeitos ósseos produzidos no olécrano de coelhos. Trinta e seis coelhos foram distribuídos em quatro grupos, recebendo como tratamento: (1) compósito de HA (49,5%), PCL (49,5%) e ALN (1%); (2) HA (50%) e PCL (50%); (3) PCL (100%); e (4) solução salina 0,9%. As amostras para análise histológica foram coletadas de três animais de cada grupo aos oito, 45 e 90 dias de pós-operatório. No oitavo dia, as bordas do defeito ainda eram identificáveis em todos os grupos. Tecido ósseo novo era formado em contato com o biomaterial apenas nas formulações que incluíam HA. Essas características continuaram evidentes nos outros momentos analisados, embora o defeito estivesse preenchido pelo tecido regenerado. A presença dos biomateriais foi verificada nos três momentos em todos os grupos. Não houve evidências de reação indesejável ao biomaterial. As análises histológicas e histomorfométricas mostraram que os biomateriais são biocompatíveis e aqueles contendo a hidroxiapatita favoreceram a formação óssea no início do processo de regeneração, embora o alendronato não tenha apresentado qualquer efeito.(AU)

Hydroxyapatite, polycaprolactone and alendronate composites for bone regeneration in rabbits' olecranon: histological features / Compósitos de hidroxiapatita, policaprolactona e alendronato na regeneração óssea em olécrano de coelhos: características histológicas

O presente trabalho teve como objetivo avaliar a biocompatibilidade e a osseointegração de compósitos de hidroxiapatita (HA), policaprolactona (PCL) e alendronato (ALN) em defeitos ósseos produzidos no olécrano de coelhos. Trinta e seis coelhos foram distribuídos em quatro grupos, recebendo como tratamento: (1) compósito de HA (49,5%), PCL (49,5%) e ALN (1%); (2) HA (50%) e PCL (50%); (3) PCL (100%); e (4) solução salina 0,9%. As amostras para análise histológica foram coletadas de três animais de cada grupo aos oito, 45 e 90 dias de pós-operatório. No oitavo dia, as bordas do defeito ainda eram identificáveis em todos os grupos. Tecido ósseo novo era formado em contato com o biomaterial apenas nas formulações que incluíam HA. Essas características continuaram evidentes nos outros momentos analisados, embora o defeito estivesse preenchido pelo tecido regenerado. A presença dos biomateriais foi verificada nos três momentos em todos os grupos. Não houve evidências de reação indesejável ao biomaterial. As análises histológicas e histomorfométricas mostraram que os biomateriais são biocompatíveis e aqueles contendo a hidroxiapatita favoreceram a formação óssea no início do processo de regeneração, embora o alendronato não tenha apresentado qualquer efeito.

Eletroacupuntura na analgesia trans e pós-operatória de cadelas submetidas à ovariosalpingohisterectomia / Electroacupuncture in trans and postoperative analgesia in bitches submitted to ovariosalpingohisterectomy

Objetivou-se com este estudo avaliar o efeito analgésico trans e pós-operatório da eletroacupuntura em onda denso-dispersa e frequências 3 e 200Hz, nos pontos E44, R3 e BP4, compará-lo com a analgesia promovida pelos pontos BP6, E36 e VB 34, e pela morfina, em cadelas submetidas à ovariosalpingohisterectomia (OSH). Teve-se como hipótese que a eletroacupuntura nos pontos E44, R3 e BP4 resultaria em controle da dor trans e pós-operatória melhor ou igual àquele promovido pela eletroacupuntura nos pontos BP6, E36 e VB34 e pela morfina. Trinta e seis cadelas submetidas à cirurgia eletiva de OSH foram distribuídas em blocos ao acaso em três grupos com 12 animais. Em dois grupos foi realizada eletroacupuntura denso-dispersa, com frequência de 3 e 200Hz, sendo que, no primeiro grupo (GEA), foram estimulados os pontos BP4, E44, R3 e, no segundo grupo (GEB), os pontos BP6, E36, VB34...

Thirty-six dogs undergoing elective ovariohysterectomy surgery were randomly distributed into 3 groups of 12 animals each. In the first group, dense-dispersed electroacupuncture was performed with a frequency of 3-200 Hz in SP4, ST44, KID3 points and 1.5mL of saline was given intramuscularly (GEA group). For the second group, dense-dispersed electroacupuncture was performed with a frequency of 3-200 Hz in SP6, ST36, GB34 points and 1.5mL of saline given intramuscularly (GEB group)...

A new surgical technique to treat corneal perforations using amniotic membrane and surgical adhesive / Nova técnica cirúrgica para tratamento de perfuração de córnea utilizando membrana amniótica e adesivo cirúrgico

The objectives of this study were to compare surgical techniques and the effects of using n-butyl 2-cyanoacrylate and bovine amniotic membrane to repair perforated lesions in corneas. Penetrating keratoplasty was performed in sixty New Zealand White rabbits under general anesthesia. Group 1 (G1) was treated with n-butyl 2-cyanoacrylate, group 2 (G2) received a fragment of amniotic membrane through the anterior chamber and application of n-butyl 2-cyanoacrylate over the lesion, group 3 (G3) was treated with the same technique as G2 with the addition of an amniotic membrane bandage covering the cornea and sutured in the limbus region, and group 4 (G4) was treated with an amniotic membrane sutured to the lesion and an amniotic membrane bandage sutured in the limbus region. Clinical, histological and histomorphometric examinations of the corneas were performed. The membrane acted as a barrier for aqueous humor in G2 and G3, thereby keeping the surface dry for adhesive application; it also prevented the adhesive from contacting intraocular structures. The groups treated with amniotic membrane and surgical adhesive showed better results than the groups treated with either material alone. Thus, the combination of the membrane with the adhesive is recommended for this type of lesion...

Os objetivos deste estudo foram comparar técnicas cirúrgicas e efeitos do n-butil 2-cianoacrilato e da membrana amniótica bovina na reparação de lesões perfuradas em córneas. Sessenta coelhos da raça Nova Zelândia Branca foram submetidos à ceratoplastia penetrante sob anestesia geral. O grupo 1 (G1) foi tratado com n-butil 2-cianoacrilato; o grupo 2 (G2) recebeu um fragmento de membrana amniótica pela câmara anterior e aplicação de n-butil 2-cianoacrilato sobre a lesão; o grupo 3 (G3) foi tratado com a mesma técnica aplicada ao G2, adicionando-se uma bandagem de membrana amniótica cobrindo a córnea e suturada à região do limbo; e o grupo 4 (G4) foi tratado com membrana amniótica suturada nas bordas da lesão e bandagem de membrana amniótica suturada na região do limbo. Foram realizados exames clínico, histológico e histomorfométrico. A membrana atuou como barreira contra o extravasamento do humor aquoso nos grupos 2 e 3, manteve a superfície seca para posterior aplicação do adesivo e impediu o contato do adesivo com as estruturas intraoculares. Os grupos tratados com o adesivo associado à membrana amniótica demonstraram melhores resultados do que aqueles tratados com cada material isoladamente. Assim, a combinação da membrana com o adesivo é indicada neste tipo de lesão...

Consenso sobre el manejo de eventos de aparente amenaza a la vida del lactante (ALTE): comisión de sueño, sociedad chilena de neumología pediátrica 2013 / Consensus on evaluation and management of apparent life-threatening events in infancy (ALTE)

Un Evento de Aparente Amenaza de la Vida o ALTE (del inglés: Apparent Life Threatening Event) corresponde a un episodio agudo que ante los ojos del observador pone en riesgo la vida de un lactante menor de 1 año. Debe presentar la combinación de 2 o más de los criterios siguientes: apnea / cambio de color / alteración del tono / atoro e implicar la necesidad de algún tipo de maniobras para reanimar. En el presente consenso sobre el manejo de un ALTE se revisaron la evidencia internacional y nacional respecto al enfoque diagnóstico, estudio etiológico, criterios y duración de hospitalización y las indicaciones de monitorización domiciliaria.

Apparent life threatening events are defined as an acute episode in which the observer fears an infant < 1 year may die. ALTE is characterized by some combination of apnea, color or muscle tone change, chocking and has to be followed by cardiorespiratory reanimation. The present consensus paper reviews international and national evidence concerning diagnosis, etiologies, hospitalization criteria and indications for home monitoring.

Prevalencia de caries temprana de la infancia en niños con enfermedades respiratorias crónicas / Prevalence of early childhood caries in children with chronic respiratory diseases

Introducción: Se ha estipulado que los niños con condiciones crónicas de salud, pueden tener un alto riesgo de caries. Aunque la literatura sobre caries tempranas de la infancia (CTI) es extensa, poco se sabe acerca de su prevalencia en niños con estas condiciones. Objetivo: Conocer la prevalencia de CTI en niños con enfermedades respiratorias crónicas (ERC) en el Hospital Calvo Mackenna, Santiago, Chile y determinar su asociación con CTI. Pacientes y Método: Se realizó un estudio de corte transversal, sobre un total de 120 niños de 2 a 5 años 11 meses de edad, un grupo de estudio de 60 niños con ERC y un grupo control de 60 niños sanos fueron analizados. Se diagnosticó CTI de acuerdo a la definición aceptada por la AAPD, mediante un examen oral realizado por un operador bajo condiciones estandarizadas. Para el análisis estadístico se utilizó el programa Stata 11. Resultados: La prevalencia de CTI fue de 53 por ciento en niños con ERC y 25 por ciento en niños sanos (p < 0,0001). El valor de Qdds Ratio para niños con ERC fue de 7,046 mostrando un incremento del riesgo de CTI, comparado con niños sanos. (95 por ciento CI: 2,372-20,929). Conclusión: En esta población, los niños con ERC presentan una alta prevalencia de CTI. En esta muestra se encontró una asociación estadísticamente significativa entre CTI y ERC.

Introduction: Children with chronic health conditions may have an increased risk for developing caries. Although the literature on early childhood cavities (ECC) is extensive, little is known about its prevalence in children with these conditions. objective: To determine the prevalence of ECC in children with chronic respiratory disease (CRD) at Luis Calvo Mackenna Hospital in Santiago, Chile, and to determine their association. Patients and Methods: A cross-sectional study was performed on 120 children aged 2 to 5 years 11 months, a study group of 60 children with CRD and a control group of 60 healthy children were analyzed. ECC was diagnosed meeting the ALAPD criteria, an oral exam conducted by an operator under standardized conditions. The Stata 11 program was used for statistical analysis. results: The prevalence of ECC was 53 percent in children with CRD and 25 percent in healthy children (p < 0.0001). The value of odds ratio for children with CRD was 7.046 showing an increased risk of ECC compared with healthy children. (95 percent CI: 2.372 to 20.929). Conclusion: In this population, children with CRD have a high prevalence of ECC. In this sample, a statistically significant association between ECC and CRD was found.

Reglas de decisión para la selección de sistemas de citas basadas en características del paciente y de la institución de salud / Decision rules to schedule patient appointments

Background:Outpatient scheduling has a significant impact on the perceived quality of service by the users and the efficient use of resources in the health system. There are mathematical methods that assist in solving this problem, but are seldom applied. Aim: To propose decision rules that are based on the own conditions of each institution and indicate which appointment system is the most suitable for the decision makers. Material and Methods: Through computer simulation, the effect of a wide range of decision and environmental factors over the appointment systems performance was assessed, in order to determine how these factors affect them. Re-suits: Consideringperformance indicators associated to the patient's satisfaction and resources utilization, scheduling shorter length patients (e.g. check-up patients) in the beginning of the working day resulted to be in the efficient solutions frontier, as well as scheduling patients in one person blocks (shifting to multiple patient blocks only if resources utilization indicators are prioritized). Conclusions: Performance indicators are more sensitive to the sequence used to schedule different length patients, rather than the number of patients scheduled per block. Moreover, decision rules based on the institution priorities are proposed, which are quite robust to environmental factors.

Condiciones sociales, factores biológicos y conducta de cuidado materno en prevención de enfermedades respiratorias en lactantes / Social conditions, biological factors and maternal care behavior in respiratory diseases prevention, in infants

Introducción: Las condiciones sociales y factores biológicos representados por variables reproductivas, clínicas, de hábitos y la conducta de cuidado materno en prevención de enfermedades respiratorias, constituyen una prioridad en el contexto socioeconómico cultural de la IX Región. Objetivos: Relacionar la conducta de cuidado materno en prevención de Infecciones Respiratorias Agudas (IRA) con la presencia de SBO y NAC. Metodología: Investigación descriptiva, correlacional, en muestra probabilística, secuencial de 239 lactantes y sus madres. Los datos se recolectaron en cuestionario validado y adaptado a las necesidades de la investigación. Se realizó análisis descriptivo y analítico, con prueba de chi cuadrado, prueba exacta de Fisher. La investigación cumplió con los Requisitos éticos de Ezequiel Emmanuel. Resultados: La conducta "evita fuentes de contagio" frecuentemente 49,8 por ciento, "ventila su hogar" frecuentemente 42,4 por ciento, "se preocupa de calefaccionar su hogar" frecuentemente 43,5 por ciento, "evita exponer al lactante a cambios bruscos de temperatura" frecuentemente 39,0 por ciento y "se siente preparada para entregar cuidados básicos de salud a su hijo" frecuentemente, 42,8 por ciento. Discusión y Conclusiones: El Síndrome Bronquial Obstructivo (SBO) se asoció a la conducta de cuidado; las madres revelaron déficit en la conducta de cuidado hacia su hijo (conducta de calefaccionar, ventilar y evitar fuentes de contagio en su hogar), lo que reafirma el propósito de generar una línea educativa dirigida a madres de lactantes con factores de riesgo.

Introduction: The social and biological factors represented by reproductive variables, clinical, and behavioral patterns of maternal care in respiratory disease prevention are a priority in the socio economic culture of our region. Objectives: Relate the maternal care behavior in prevention of acute respiratory infections (ARI) with the presence of SBO and NAC. Methodology: Descriptive and correlational research with a probability sequence sample of 239 infants and their mothers. A validated questionnaire and adapted to the needs of the investigation, was used for data collection. Descriptive and analytical study with chi-square test and Fisher exact was carried out. Research complied with the ethical requirements of Ezekiel Emanuel. Results: They indicated that the behavior "avoid sources of infection", frequently reaches 49.8 percent, "home fan" 42.4 percent, "cares about heating your home" 43.5 percent, "avoid exposing the infant to sudden temperature changes" 39.0 percent and "feels ready to deliver basic health care to your child", 42.8 percent. Discussion and Conclusions: Bronchial obstructive syndrome (BOS) was associated with protective behavior, mothers showed deficits in behavior toward their child care (behavior of heating, ventilation and avoid sources of infection at home) which reaffirms the purpose of generating an educational line, for mothers of infants with risk factors.

Perfil socio-familiar en adultos mayores institucionalizados: Temuco, Padre Las Casas y Nueva Imperial / Socio-family profile in institutionalized older adults: Temuco, Padre Las Casas and Nueva Imperial

Introducción: El proceso de envejecimiento genera cambios importantes en el estilo de vida de la población con repercusiones significativas en el volumen y distribución de la carga social de enfermedad. La Organización de Naciones Unidas (ONU) plantea que es un fenómeno secuencial, acumulativo e irreversible, que deteriora el organismo progresivamente hasta hacerlo incapaz de enfrentar circunstancias y condiciones del entorno. Objetivo general: Conocer el perfil socio-familiar de adultos mayores institucionalizados en comunas de Temuco, Padre Las Casas y Nueva Imperial, año 2009. Metodología: Estudio descriptivo transversal correlacional, en muestra de 80 adultos mayores institucionales. La recolección de la información se realizó con el Cuestionario Calidad de Vida para Ancianos, elaborado por Inga y Vara, adaptado a los objetivos del estudio. Se realizó análisis descriptivo (distribución de frecuencias, medias, promedios-desviación estándar) e inferencial (chi cuadrado, Pearson). Resultados: El promedio de edad fue de 78,1 años, 15,0 por ciento eran mapuches, 55,0 por ciento eran masculinos, 11,2 por ciento "no sabe leer ni escribir" , 40,5 por ciento no tiene contacto con sus hijos, en maltrato ha recibido golpes o empujones (15,1 por ciento más de diez veces).18,7 por ciento piensa casi a diario que está insatisfecho con su vida actual, 59,4 por ciento no recibe atención de salud por la institución. Conclusiones: se constató una situación de aislamiento, soledad personal y déficit de cuidados entregado por las instituciones, reto para Enfermería, donde debería tener participación de privilegio en la toma de decisiones en políticas de cuidado para la población en estudio.

The aging process produces important lifestyle changes in the population with a significant impact on the volume and distribution of the social burden of disease. The United Nations (UNO) suggests that it is a sequential, cumulative and irreversible phenomenon beginning at age 60 where the human being experiences physical, emotional and social changes which progressively damage the body until it is incapable of dealing with environmental circumstances and conditions. Objective: To learn about the socio-family profiles of institutionalized older adults in Temuco, Padre Las Casas, and New Imperial during the year 2009. Specific objectives: To identify individual characteristics - family, social status, social relationships, satisfaction with life, general health status, negative memories, level of family and social dependency, institutional attention and type of relationship with the consumption of alcoholic beverages in institutions in the towns of Temuco, Padre Las Casas and New Imperial. Methodology: A cross-sectional correlation study was carried out with a sample of 80 institutionalized older adults. Data collection was carried out with the Quality of Life Questionnaire validated for older adults, as elaborated by Inga and Vara and adapted to the study objectives. Descriptive analyses were carried out (with frequency distribution, means, averages, standard deviation, as well as inferential statistics (with Pearson’s chi-squared Test). Results: The average age was 78.1 years old, 15.0 percent were Mapuche, 55.0 percent were male, 11.2 percent were unable to read or write, 32.5 percent were single. 18.7 percent received no pension; 40.5 percent had no contact with their children and were abused having been hit or pushed (15.1 percent more than ten times). 52.5 percent never engaged in physical activity or played or worked with friends (gardening, errands, strolls, games of chance, etc.). In relationship to life satisfaction (18.7 percent thought almost...

Infecciones de catéter venoso central y lock terapia en pacientes oncológicos / Infections of the central line and lock therapy in pediatric oncological children

Introduction: The use of a permanent Central Venous Catheter (CL) in oncologic patients allows a secure and rapid access. Infection is an important risk. "Lock therapy" (LT) consists of the application of a high concentration of antibiotics in the lumen of the catheter, for the purpose of eradicating bacteria. Objective: To describe the use of LT associated with the systemic use of antibiotics in CL infections occurring among pediatric oncological patients. Methods: A descriptive, retrospective review of charts of children seen at the Oncology Unit, Roberto del Rio Children's Hospital, 2005 to 2007. Evaluated parameters included culture and sensitivity studies, removal of CL and mortality. Results: There were 11 episodes of infection associated to CL in 8 patients, most frequently associated to Staphylococcus coagulase-negative. The CL was preserved in 7 patients. There was no mortality associated to infection. Conclusion: For this group, LT and systemic antibiotics preserved the CL in most patients. Randomized prospective studies are needed in a larger population to support these findings.

Introducción: El uso de catéter venoso central permanente (CVC) en pacientes oncológicos aporta un acceso venoso seguro y expedito. Sin embargo, la infección es una de las principales complicaciones asociadas a su uso. La "lock terapia" es una técnica que consiste en la aplicación en el lumen del catéter de una solución con alta concentración de antibiótico, con el objetivo de erradicar las bacterias presentes en el endolumen. Objetivo: Describir el uso de "lock terapia" asociada a antibióticos sistémicos en infecciones relacionadas a CVC en pacientes pediátricos oncológicos. Métodos: Estudio descriptivo, retrospectivo, donde se analizaron las infecciones asociadas a CVC que fueron tratadas con lock terapia y antibióticos sistémicos en la Unidad de Oncología del Hospital de Niños Roberto del Río entre los años 2005 y 2007. Los parámetros estudiados fueron: cultivo y antibiograma; retiro del CVC y mortalidad. Resultados: Se estudiaron 11 episodios de infección asociada a CVC en 8 pacientes. El microorganismo aislado con mayor frecuencia fue Staphylococcus coagulasa negativo. Se logró conservar el CVC en 7 pacientes. No hubo mortalidad asociada a infección. Conclusiones: En el grupo estudiado, el uso asociado de lock terapia y antibióticos sistémicos permitió conservar el CVC en la mayoría de los casos. Se requieren estudios prospectivos randomizados con un mayor número de pacientes que permitan corroborar estos hallazgos.

Talla baja idiopática y uso de hormona de crecimiento: documento del consenso de la Sociedad Chilena de Endocrinología y Diabetes / Consensus recommendations of the Chilean Society of Endocrinology an Diabetes on growth hormone use in idiopathic short stature

The diagnosis of idiopathic short stature (ISS) is common among patients with short stature, especially those with a height lower than 2 standard deviations (SD) of the mean. The diagnosis of ISS is considered in children with short stature in whom no recognizable causes are found after a proper evaluation by pediatric endocrinologists. The professional must perform a complete personal and family history, appropriate anthropometry and physical examination and confirm that general and specific laboratory studies including supraphysiological stimuli to measure growth hormone, are normal. Growth hormone (GH) treatment is safe and effective in patients with ISS. Its effects are very similar to those observed in other conditions that affect growth as Turner Syndrome and Small for Gestational Age Short Children. However, treatment is still controversial because ethical, psychological, social, cultural and economic issues, wich are difficult to evaluate, must be taken into account. Individual patient differences and their family environment must also be considered. The hormone is more often indicated to fulfil parent or social environment needs rather than the wish of the patients. Although the treatment is safe, it is not free of complications and its results are often poorer than those expected by patients or their parents. The Chilean Society of Endocrinology and diabetes commissioned a panel of experts among its members, to generate a consensus document on ISS and the use of growth hormone, to provide information and recommendations to the Chilean community.

Síndrome metabólico en niñas púberes con hiperplasia suprarrenal congénita clásica / Metabolic syndrome among puberal girls with classic congenital adrenal hyperplasia

Background: Adult women with adrenal congenital hyperplasia (AH) have a higher risk for insulin resistance, dyslipidemia, hypertension, high body mass index (BMI) and increased body fat. All these factors are associated with cardiovascular risk and metabolic syndrome (MS). Aim: To evaluate the presence of MS in pubertal classic AH girls (CAH) and a control group (C). Material and Methods: We studied 15 pubertal AH patients (12.0 +/- 1.9 years) and 26 controls (11.7+/- 0.3 years) matched by age and tanner stage. Weight, height, BMI, waist/hip ratio, blood pressure and serum lipids were measured. An oral glucose tolerance test (OGTT) and insulin curve was performed in CAH girls whereas in controls basal insulin and glucose were determined. The homeostasis model assessment for insulin resistance (HOMAIR) was calculated. Cook, Ferranti and international diabetes federation (IDF) criteria were used to determine the presence of MS. Results: CAH and C girls had similar BMI (22.0 +/- 5.1 and 20.1 +/- 3.6 kg/m2 respectively; p = 0,11). CAH girls had higher basal blood glucose (80.8 +/- 7.7 and 60.6 +/- 10.6 mg/dl respectively, p < 0.01) and controls had higher triglyceride levels (147.0 +/- 69.3 and 79.7 +/-16.3 mg/dl respectively, p < 0.01) and lower HDL cholesterol levels (45.8 +/- 12.8 and 56.9 +/- 17.5 mg/dl respectively, p = 0.02). According to cook criteria 4 percent of CAH girls and 23 percent of controls has MS. These figures were 14 and 32 percent respectively according to Ferranti criteria and 0 and 5 percent respectively according to IDF criteria. Conclusions: CAH puberal patients do not have a higher prevalence of metabolic syndrome, compared with controls with similar Tanner stage and BMI.