1.
Arq. bras. med. vet. zootec
;
61(4): 986-991, ago. 2009. mapas, tab
Article
in Portuguese
| LILACS
| ID: lil-524456
2.
Arq. bras. med. vet. zootec
;
57(5): 710-714, out. 2005. ilus
Article
in Portuguese
| LILACS
| ID: lil-418854
ABSTRACT
The immunohistochemistry standardization for bovine viral diarrhea virus (BVDV) diagnostic was described. The formalin-fixed tissue samples from a heifer with mucosal disease were used as positive control. The validation of the first phase results was performed using samples from an aborted fetus and a calf infected with reference strains of BVDV. The best results were seen using monoclonal antibodies and a commercial kit consisting of labelled streptavidin biotin (LSAB) reagents and the diaminobenzidine (DAB) substrate-chromogen reagent. The immunohistochemistry demonstrated to be an useful method for routine diagnosis for the controll and detection of BVDV infection.