ABSTRACT
The aim of this investigation was conducted to proteomic analysis of plasma obtained from pregnant women who destined to develop late-onset preeclampsia without intrauterine growth restriction [IUGR] during 16[th] week of gestation. Plasma was obtained from primiparous women during 16[th] week of gestation. 2-DE proteomic analysis was done for plasma from 11 healthy pregnant women and 11 women who developed preeclampsia later. Using bioinformatic analysis with Progenesis SameSpots ver4.0 software and ANOVA test, expression of 2 spots were statistically different between two groups. In preeclamptic state, expression of both were decreased, one of these spots was vitamin D binding protein [p-value: 0.047], the other one will be discussed in another paper. According to results, we concluded that during 16[th] week of gestation, occurance of late-onset preeclampsia without IUGR is predictable. During this week, pathology of disease is present and may be the process of placental degeneration and impaired placentation are include in disease pathology
ABSTRACT
The aim of this study was to compare three routine precipitation methods, including: acetone, TCA/acetone wash and TCA/acetone. 30 plasma samples were precipitated using above mentioned three methods. Pellets were dissolved in rehydration solution and protein quantification was done for this solution. According to statistical analysis using SPSS ver 16.0 software and one-way ANOVA test, the protein yield of acetone method was greater than two other methods and was statistically different from TCA/acetone precipitation method [p-value: 0.006]. The acetone method is more efficient than two other methods in protein precipitation