Comparison of volar and dorsal approaches for surgical treatment in fracture of proximal half of the radius

Objective: Fracture of the proximal half of the radius shaft can be exposed by either one of volar or dorsal approaches. The aim of this study was to compare the results of volar and dorsal approach for surgical treatment of proximal half fracture of the radius

Methodology: This prospective study was performed from April 2008 to March 2012 in two teaching hospitals. Seventy adults patients with closed fracture in proximal half of the radius or radius and ulna were operated on and fixed using small plate and screw by volar approach [VA] [39 patients] and dorsal approach [DA] [31 patients]. Comparison of the results in both surgical approach were the primary measurement outcome. Duration and time of procedure, rate and time of fracture union and motion of the forearm were assessed at 4 months after operation

Results: Mean age of the patients with VA and DA was 25.3 and 26.5 years respectively. There was 26 male and 13 female in VD and 22 male and 9 female in DA patients. Radial nerve injury in VA and DA occurred in three and two patients, infection in one and nonunion in one other patient was seen in each group. There was no significant difference in duration of procedure or time of union after both approaches =0.643. Mean rotation of forearm was 135 deg. in VA, and 138 deg. in DA patients at 4 months post surgery

Conclusion: There was no significant difference in term of fracture union, early complications, and range of forearm rotation between volar and dorsal approach for the fixation of radius fractures in its proximal half

Comprehensive phylogenetic, similarity and allergenicity analysis of Boophilus genus tick Tropomyosin protein

Boophilus genus ticks are responsible for transferring some pathogens and reducing production factors in cattle. Tropomysin [TPM] protein has actin regulator activity and playing important role in immune and allergic reactions. The main goal is to determine different aspects of phylogenetic, similarity, homology, structure and allergenicity of TPM protein. In prior study, we identified TPM by using Mass-spectrometry in Boophilus anulatus larva proteins extraction. Analysis by NCBI and Mascot software showed complete similarity of this protein with Boophilus microplus. TPM Blasting, invertebrates TPM sequences retrieval, aligning and analyzing of conserved and variable regions along sequences were next steps. Also, construction the phylogenetic tree, overall mean distances estimation, homology protein secondary structure, allergencity analysis was achieved. The most similar sequences to Boophilus genus TPM are Haemaphysalis sp., Scolopendra sp. and etc., respectively. The multiple sequence alignment showed that conserved and variable regions stretched in different part of TPM. The close relationships in Phylogenetic tree between Ticks and Mites were seen, although the TPM sequences in ticks are more similar to each other than to mites and assume as the nearest relatives. Insects TPM like worms, located in two separated clades, and Trichinella spiralis in worm clades are more related taxa to members of ticks and mites groups. Furthermore, overall mean distances over sequence pairs reflects TPM conservation during speciation. TPM has high homology in different species and has two domain of ?-helix that cannot form disulfide bonds. Finally, allergenicity analysis by separated and hybrid approach showed it undoubted is allergen and candidates some peptides as responsible for allergenicity of TPM. The comprehensive analysis of TPM has never been easy, especially when we attempt to make statements from different aspects about this protein. Our study revealed the some unique and valuable aspects of TPM protein of Boophilus genus, and will help to further studies on mentioned protein

Bacteriophage as a novel antibacterial agent in industry and medicine

Bacteriophage is a kind of virus that infect bacteria, and is distinct from the animal and plant viruses that could have either lytic or lysogenic cycle. Lytic phages known as candidates for phage therapy, since they rapidly replicate into their host and lyse them. Theoretically, phages have more than a few benefits over routine antibiotics. They are extremely specific and for their targeted hosts and also are safe for human, because they have no activity against eukaryote cells. Over this time, phages were used to treat various infections. Although, phages have a lot of advantages against antibiotics, their industrial production as a commercial production have been ceased in most of the western European countries. These days, by raising of antibiotics resistance and inefficiency of antibiotic to overcome bacteria biofilm, there is renewed global attention in phage applications as a potentially powerful antibacterial agents. Different published paper through the world indicates bacteriophage could be recruited as suitable agent for therapeutic purposes in medicine and food industry. Therefore, here we tried to review most of these ideas about phage application as a rapid review