BARRIERS AND CHALLENGES FACED BY SCHEDULED TRIBE SECONDARY SCHOOLS STUDENTS: A STUDY OF DISTRICT POONCH

Education of Scheduled Tribes is of particular importance in the perspective of country planning and resource development as it is one of the primary agents of change towards development and determines employment prospects. Lack of education of Tribal students is a major concern, as they lag behind their socio-economic, cultural and educational background. The investigator in current tries to investigate the social- economic and personal barriers faced by tribal students in Poonch district of Jammu and Kashmir with respect to gender. The information related problems is obtained through the self-constructed questionnaire, scheduled tribe secondary school students are the participant for the present study. The study depicted a noteworthy variance among the male and female students; female scheduled tribe's students have faced more Social, Economic and Personal problems as compare to their male counterparts

Efeito do treinamento pliométrico de curta duração no salto vertical e velocidade de sprint em jogadores de vôlei / Effect of short duration plyometric training on vertical jump and sprint speed in volleyball players

INTRODUÇÃO: O voleibol é um esporte de ritmo acelerado que envolve projeções verticais e horizontais da bola por parte do jogador. O uso de treinamento pliométrico é um método popular para melhorar o desempenho dos jogadores. OBJETIVO: O presente estudo visa verificar o efeito de quatro semanas de treinamento pliométrico em jogadores de voleibol. MÉTODO: Estudo randomizado, controlado, de grupos paralelos. Foram triados 39 participantes para participação no estudo, dos quais 30 foram selecionados seguindo os critérios de inclusão e exclusão da pesquisa. Os critérios de inclusão foram: jogadores de voleibol na faixa etária de 18 a 24 anos, de ambos os sexos, experiência de jogo de no mínimo um ano e disponibilidade para participar. Os critérios de exclusão compreendiam jogadores com histórico de lesão nos últimos seis meses, qualquer distúrbio neurológico ou musculoesquelético e qualquer condição psicológica. Os participantes selecionados foram alocados aleatoriamente no Grupo A e Grupo B usando o método de sorteio. O Grupo A recebeu treinamento pliométrico por três dias em uma semana, e o Grupo B foi solicitado a continuar seus exercícios regulares de rotina. A duração total da intervenção foi de quatro semanas. A medida de resultado para o estudo foi a velocidade do sprint e a altura do salto vertical. A velocidade de sprint foi medida pelo teste de sprint de 20 m e a altura do salto vertical foi medida pelo teste sargent jump. A avaliação foi feita no início e no final de quatro semanas. O teste t não pareado foi usado para analisar as diferenças entre os grupos e o teste t pareado foi usado para analisar as diferenças dentro do grupo nas variáveis de resultado. RESULTADOS: O resultado do estudo mostrou uma melhora significativa na altura do salto vertical (MD= -7,133, IC 95% (-12,657,- 1,609) e nenhuma melhora significativa na velocidade do sprint quando comparações entre grupos foram feitas (MD=0,084, 95% IC (-0,177,-,345) com um tamanho de efeito de 0,75 para a altura do salto vertical. A comparação dentro do grupo foi considerada significativa apenas para a altura do salto vertical no Grupo A, e não houve melhora significativa na velocidade de sprint para ambos Grupo A e B. CONCLUSÃO: Quatro semanas de programa pliométrico é eficaz em melhorar a altura do salto vertical e não a velocidade de sprint em jogadores de voleibol.

INTRODUCTION: Volleyball is a fast-paced sport that involves vertical and horizontal projections of the ball by the player. The use of plyometric training is a popular method to enhance performance in players. OBJECTIVE: The present study aims to see the effect of short-duration plyometric training on vertical jump and sprint speed in volleyball players. METHOD: This study is a parallel group randomized controlled trial. Thirtynine participants were screened for participation in the study, of which 30 were selected following the inclusion and exclusion criteria for the study. The inclusion criteria for the study comprised of volleyball players of age group 18 to 24 years, both males and females, with playing experience of at least one year, and willing to participate. The exclusion criteria for the study included players with a history of injury in the last six months, any reported neurological or musculoskeletal disorder, and any psychological condition. The selected participants were randomly allocated to Group A and Group B using the lottery method. Group A was given plyometric training for three days a week and Group B was asked to continue their regular routine exercises and playing schedule. The total duration of the intervention was four weeks. The outcome measure for the study was sprint speed and vertical jump height. Sprint speed was measured by the 20 m sprint test, and the vertical jump height was measured by the Sargent jump test. The assessment was done at the baseline and at the end of four weeks. An unpaired t-test was used to analyze between-group differences, and paired t-test was used to analyze the within-group differences in the outcome variables. The significance level was set as p<0.05. RESULTS: The result of the study showed a significant improvement in vertical jump height (MD= -7.133, 95% CI-12.657, -1.609) and no significant improvement in sprint speed when between-group comparisons were made (MD=0.084, 95%CI -0.177,-.345) with an effect size of 0.75 for vertical jump height. The within-group comparison was found significant only for vertical jump height in Group A, and there was no significant improvement was found in sprint speed for both Group A and B. FINAL CONSIDERATIONS: The short-duration plyometric training is effective in improving the vertical jump height and not the sprint speed in volleyball players. The specificity of plyometric training is important for optimal improvement in sports performance.

Perceived esthetics and value of clear aligner therapy systems: A survey among dental school instructors and undergraduate students

ABSTRACT Objective: To investigate the attractiveness, acceptability, visibility and willingness-to-pay for clear aligner therapy (CAT) systems in first-year and final-year dental students and instructors. Methods: A questionnaire designed to collect information regarding esthetic preferences and intentions related to seven CAT systems was handed out to 120 undergraduate students and instructors at the Academic Centre for Dentistry Amsterdam (ACTA). Proportional odds models and population average generalized estimating equation models were used to examine potential association between participant characteristics, esthetic perceptions and CAT systems. Results: Overall, the examined CAT systems received favorable esthetic ratings. Expertise status was significantly associated with willingness-to-pay additionally for CAT, compared to fixed orthodontic appliances. There was no association between sex, previous orthodontic treatment history, satisfaction with own dental appearance and potential interest in treatment and aligner visibility and willingness-to-pay. CAT system was significantly associated with the perceived aligner visibility, acceptability and attractiveness by students and instructors. Conclusions: CAT systems were considered to a great extent attractive and acceptable for future treatment by dental school instructors and students. Willingness-to-pay for CAT systems was significantly associated with expertise status, with instructors appearing more reluctant to pay for CAT.

RESUMO Objetivo: Comparar diferentes sistemas de tratamento com alinhadores transparentes (CAT), quanto à atratividade, aceitabilidade, visibilidade e disposição a pagar, por parte de alunos (primeiro e último anos) e instrutores de Odontologia. Métodos: Um questionário elaborado para coletar informações sobre preferências e intenções estéticas, em relação a sete sistemas CAT, foi distribuído para 120 alunos de graduação e instrutores do Academic Centre for Dentistry Amsterdam (ACTA). Modelos de riscos proporcionais e modelos de equação de estimação generalizada para a média da população foram usados para examinar a possível associação entre as características dos participantes, percepções estéticas e os sistemas CAT. Resultados: No geral, os sistemas CAT examinados receberam avaliações estéticas favoráveis. O nível de experiência foi significativamente associado com a disposição em pagar mais por sistemas CAT do que por aparelhos ortodônticos fixos. Não houve associação entre sexo, histórico de tratamento ortodôntico anterior, satisfação com a própria aparência dentária, potencial interesse em tratamento, visibilidade do alinhador e disposição em pagar mais. Os sistemas CAT foram significativamente associados à visibilidade percebida, aceitabilidade e atratividade dos alinhadores por alunos e instrutores. Conclusões: Os sistemas CAT foram considerados, em grande parte, atraentes e aceitáveis para tratamentos futuros pelos instrutores e alunos do curso de Odontologia. A disposição em pagar mais pelos sistemas CAT foi significativamente associada ao nível de especialização, com os instrutores parecendo mais relutantes em pagar mais pelo CAT.

A restrição de alimentos lácteos é obrigatória para pacientes com doença inflamatória intestinal? Estudo transversal multinacional / Is dairy foods restriction mandatory for inflammatory bowel disease patients: a multinational cross-sectional study

ABSTRACT Background: The role of dairy foods in inflammatory bowel disease (IBD) has been controversial and it is debatable if patients with IBD should avoid milk and dairy products or not, as well as the relationship between these foods and symptoms among those population. Objective: This multi centric cross-sectional study designed to evaluate if it is really necessary to deprive IBD patients from consumption of dairy foods. Methods: A multicenter study with 12 gastroenterology referral centers in four countries was designed to evaluate gastrointestinal (GI) symptoms after consumption of dairy foods from all outpatients with IBD during 6 months and to compare patients treated at the same centers without IBD (non IBD cases). Results: Overall 1888 cases included (872 IBD patients and 1016 non IBD cases). 56.6% of participants were female with average age of 40.1 years. Racially 79.8% participants were Caucasians and originally they were citizens of 10 countries. Relative prevalence of IBD was higher in Africans and Indians and the most frequent prevalence of dairy foods intolerance was seen in Asians. Among IBD patients, 571 cases diagnosed as ulcerative colitis and 189 participants as Crohn's disease. Average duration of diagnosis as IBD was 6.8 years (from 2 months to 35 years). The most prevalent GI symptoms after consumption of all the dairy foods were bloating and abdominal pain. Totally, intolerance of dairy foods and lactase deficiency was more prevalent among IBD patients in comparison with non IBD cases (65.5% vs 46.1%, P=0.0001). But the rate of GI complains among IBD patients who had not any family history of lactase deficiency, history of food sensitivity or both were 59.91%, 52.87% & 50.33% respectively and similar to non IBD cases (P=0.68, 0.98 & 0.99 respectively). Conclusion: The rate of dairy foods intolerance among IBD patients without family history of lactase deficiency or history of food sensitivity is similar to non IBD cases and probably there is no reason to deprive them from this important source of dietary calcium, vitamin D and other nutrients.

RESUMO Contexto: O papel dos alimentos lácteos na doença inflamatória intestinal (DII) tem sido controverso e é discutível se os pacientes com DII devem ou não evitar leite e laticínios, bem como a relação entre esses alimentos e sintomas nesta população. Objetivo: Estudo transversal multicêntrico foi projetado para avaliar se é realmente necessário privar os pacientes com DII do consumo desta classe de alimentos. Métodos: Um estudo multicêntrico com 12 centros de referência em gastroenterologia de quatro países foi projetado para avaliar sintomas gastrointestinais após o consumo de alimentos lácteos em todos os ambulatórios de DII durante seis meses e comparar pacientes tratados nos mesmos centros sem DII. Resultados: No total, foram incluídos 1888 casos (872 pacientes com DII e 1016 casos sem DII. 56,6% dos participantes eram do sexo feminino com idade média de 40,1 anos. 79,8% dos participantes eram caucasianos e originalmente eram cidadãos de 10 países. A prevalência relativa de DII foi maior em africanos e indianos e a prevalência mais frequente de intolerância a alimentos lácteos observada nos asiáticos. Entre os pacientes com DII, 571 casos foram diagnosticados como colite ulcerativa e 189 participantes como doença de Crohn. A duração média do diagnóstico como DII foi de 6,8 anos (de 2 meses a 35 anos). Os sintomas de gastrointestinais mais prevalentes após o consumo de todos os alimentos lácteos foram inchaço e dor abdominal. No total, a intolerância aos alimentos lácteos e a deficiência de lactase foi mais prevalente entre os pacientes com DII em comparação com os casos sem DII (65,5% vs 46,1%, P=0,0001). A taxa de queixas gastrointestinais entre os pacientes com DII que não tinham histórico familiar de deficiência de lactase, histórico de sensibilidade alimentar ou ambos foram de 59,91%, 52,87% e 50,33% respectivamente e semelhantes aos casos sem DII (P=0,68, 0,98 e 0,99, respectivamente). Conclusão: A taxa de intolerância de alimentos lácteos entre pacientes com DII sem histórico familiar de deficiência de lactase ou histórico de sensibilidade alimentar é semelhante aos casos sem DII e provavelmente não há razão para privá-los dessa importante fonte de cálcio dietético, vitamina D e outros nutrientes.

ACHIEVEMENT MOTIVATION AND ACADEMIC ACHIEVEMENT OF SECONDARY SCHOOL STUDENTS: AN EMPIRICAL STUDY OF JAMMU AND KASHMIR

Motivation is the motivating force behind someone's behaviour and actions in life. Different desires or rewards empower different types of people, so trying to identify someone's motivators can certainly assist individuals to employ them for bene?t. The core objective of the present study was to investigate relationship among the academic and achievement motivation with respect to gender. Data was gathered from 240 secondary school students various secondary schools in Jammu and Kashmir UT. An equal number of boys and Girls are selected by a simple random method. For Achievement Motivation, Mukherjee's Achievement Test was used for data collection, and for Academic Achievement was measured through the marks obtained in a previous class by the students. The ?nding of the study shows there is positive correction between the academic motivation and achievements motivation. In addition, the study also reveals the males shows signi?cant positive relationship as compare to their counterpart.

Maternal Outcome of Eclamptic Patients Admitted in Gazi Medical College Hospital

Background: Eclampsia is one of the major causes of maternal and perinatal mortality. Eclampsia is more common in developing countries compared to developed countries.This study aimed to assess the maternal outcome in eclamptic patients.Material & Methods:This cross-sectional study was conducted in the Department of Obstetrics and Gynecology, Gazi Medical College, Khulna, Bangladesh, during the period from September 2017 to February 2019.Results:The mean age of study people was 23.84 years (SD±5.03 years), among them 58% had postpartum eclampsia and 42% had antepartum eclampsia. Most of the study people (62%) did not take regular antenatal check-ups during pregnancy and 47% of study people had 1-4 convulsions before admission, and most of the study people (80%) had high blood pressure stage 2 (?140/?90 mmHg), Oedema was presented in 98% of study people, 44% of study people and 3gm/l(+++) albumin in the urine. In this study, 56 % had LUCS delivery and 44% had a vaginal delivery, 7% of study people had pulmonary oedema, 7% had renal failure, 2% had DIC, 7% had CVA, 9% had abruption placenta 16% had PPH, 31% needed admission in ICU. There was no maternal death, mean duration of hospital stay was 7 days (SD±3 days). For most of the study people (56%), BP on discharge was normal (?120/?80 mmHg) and for all study people, urine albumin was nil.Conclusions:Eclampsia was a major cause of maternal and fetal mortality and morbidity in Bangladesh. Race and age appear to be risk factors for eclampsia with Bangladeshi women and those at the extremes of reproductive age at greater risk. Antenatal care is important in reducing perinatal mortality and possibly maternal complications.

Atividade física na gravidez e seu efeito sobre os parâmetros relacionados ao peso: um estudo piloto randomizado e controlado / Physical activity in pregnancy and its effect on weight-related parameters: a pilot randomized controlled trial

INTRODUÇÃO: O declínio no nível de atividade física durante a gravidez está relacionado a várias complicações relacionadas à gravidez. Portanto, a atividade física regular durante a gravidez tem um papel importante na manutenção da boa saúde reprodutiva e de um estilo de vida saudável. OBJETIVOS: Avaliar o efeito de exercícios supervisionados, mensagens de texto e intervenções de atividade física baseadas em pedômetros durante a gravidez sobre os níveis de atividade física e parâmetros relacionados ao peso. MATERIAIS E MÉTODOS: Estudo piloto de grupo paralelo controlado e aleatório. Sessenta mulheres grávidas foram aleatorizadas em cinco grupos (N=12 em cada grupo): Grupo A: Exercício supervisionado; Grupo B: Pedômetro; Grupo C: Pedômetro mais mensagem de texto; Grupo D: Mensagem de texto e Grupo E: Controle. Mulheres grávidas de 20 a 30 anos de idade com uma gravidez de um botão e idade gestacional inferior a 16 semanas na inclusão, um IMC ≥ 18,5 kg /m2, uma disponibilidade de telefone celular e capacidade de falar e ler hindi e inglês foram selecionadas para participação no estudo. Mulheres com gestações gêmeas ou múltiplas, IMC >30kg/m2, gravidez de alto risco conforme decisão do ginecologista, quaisquer complicações relacionadas à gravidez, tais como hipertensão pré-gestacional, diabetes pré-gestacional e outras condições de saúde importantes que restringem sua atividade física no momento do recrutamento e mulheres grávidas analfabetas foram excluídas do estudo. O Grupo A recebeu um exercício supervisionado de luz a moderada intensidade de 45-60 minutos uma vez por semana a partir da 15ª semana até o parto. Os grupos B e C foram encorajados a melhorar o nível de atividade física, concentrando-se em uma contagem de passos de pelo menos 5000-7500 passos por dia avaliados por pedômetro em sete dias consecutivos por mês. O grupo C, além do pedômetro e o grupo D receberam SMS padrão relacionados aos cuidados da gravidez (atividade física, dieta, motivacional e educacional específica). Foi utilizada a ANOVA de uma via para estimar as diferenças entre os grupos e foi utilizado o teste t pareado para estimar a diferença dentro do grupo nas variáveis de resultado (p=0,05). RESULTADOS: O resultado do estudo mostrou estatisticamente significativo entre as diferenças de peso dos grupos no 9º mês (p=0,029) e a retenção de peso nos 2 meses pós-parto (p=0,005). O grupo de exercício supervisionado reteve menos peso durante o período pós-parto em comparação com o grupo de controle (Exercício supervisionado vs Controle, MD = 2,79kg, p=0,002). Entretanto, não houve melhora estatística significativa no ganho de peso gestacional, IMC, circunferência da cintura, circunferência do quadril e relação cintura/quadril em comparação com os grupos. CONCLUSÃO: Pode-se concluir que os exercícios supervisionados são eficazes para aumentar a atividade física, reduzir os parâmetros relacionados ao peso e ajudar na adoção de um estilo de vida saudável durante a gravidez.

INTRODUCTION: The decline in the level of physical activity during pregnancy is related to various pregnancy related complications. Therefore, regular physical activity during pregnancy plays an important role in maintaining good reproductive health and healthy lifestyle. OBJECTIVES: was to assess the effect of supervised exercises, text messages, and pedometer-based physical activity interventions during pregnancy on physical activity levels and weight-related parameters. MATERIALS AND METHODS: Controlled, randomized, parallel-group pilot study. Sixty pregnant women were randomized into five groups (N= 12 in each group): Group A: Supervised exercise; Group B: Pedometer; Group C: Pedometer plus text message; Group D: Text message and Group E: Control. Pregnant women aged 20-30 years with a singleton pregnancy and gestational age of less than 16 weeks at inclusion, a BMI ≥ 18.5 kg /m2, an availability of mobile phone and ability to speak and read Hindi and English languages were selected for participation in the study. Women with twin or multiple pregnancies, BMI >30kg/m2, high-risk pregnancy as decided by the gynecologist, any pregnancy related complications such as pre-gestational hypertension, pre-gestational diabetes, and other major health conditions restricting their physical activity at the time of recruitment and illiterate pregnant women were excluded from the study. Group A received supervised light to moderate intensity exercise of 45-60 minutes once weekly from the 15th week till delivery. Groups B and C were encouraged to improve physical activity level, focusing at a steps count of at least 5000-7500 steps per day assessed by pedometer on seven consecutive days per month. Group C in addition to pedometer and group D received standard SMS related to pregnancy care (physical activity, diet, motivational, and educational specific. One-way ANOVA was used to estimate the between-group differences and Paired t-test was used to estimate the within-group difference in the outcome variables (p=0.05). RESULTS: The result of the study showed statistically significant between groups differences in the weight at 9th month (p=0.029) and weight retention at 2months post-partum (p=0.005). The supervised exercise group retained less weight during post-partum period as compared to control group (Supervised exercise vs Control, MD = 2.79kg, p=0.002). However, there was no statistical significant improvement in gestational weight gain, BMI, waist circumference, hip circumference and waist to hip ratio as compared to groups. CONCLUSIONS: It can be concluded that supervised exercises are effective in increasing physical activity, reducing weight-related parameters, and help in adopting a healthy lifestyle during pregnancy.

Um estudo randomizado controlado sobre a eficácia da diatermia de ondas longas na dor, incapacidade e amplitude de movimento em pacientes com dor cervical / A randomized controlled trial on the efficacy of longwave diathermy on pain, disability and range of motion in the patients with neck pain

INTRODUÇÃO: A cervicalgia é uma importante causa de incapacidade em todo o mundo, causada por várias condições como doenças de base, anormalidades mecânicas e neuropáticas. A Diatermia por Ondas Longas (LWD) é uma modalidade de aquecimento terapêutico usada para tratar muitas condições musculoesqueléticas. OBJETIVO: Avaliar a eficácia da LWD na dor, incapacidade e amplitude de movimento (ADM) na dor no pescoço. MÉTODOS E MATERIAIS: O estudo foi conduzido após aprovação ética da universidade e registro em registro de ensaio clínico (PTY/2022/155 e CTRI/2022/06/043033). 30 pacientes com cervicalgia de 20-60 anos foram alocados aleatoriamente em dois grupos. O grupo controle (n=15) realizou exercícios domiciliares simples, enquanto no grupo experimental (n=15) a diatermia de ondas longas também foi administrada juntamente com exercícios domiciliares, 3 vezes/semana por 2 semanas. As medidas de resultado, como a escala visual analógica (EVA), índice de incapacidade do pescoço (IIP) e amplitude de movimento do pescoço, foram avaliadas na linha de base, no final de 2 semanas (pós-tratamento) e após um acompanhamento de 2 semanas. RESULTADOS E CONCLUSÃO: Houve melhora significativa da dor, incapacidade e ADM pós-intervenção no grupo controle e no grupo experimental. Além disso, houve diferença significativa na dor após o acompanhamento em ambos os grupos. A comparação entre os grupos sugeriu que houve uma diferença significativa para EVA, IIPe ADM de extensão do pescoço (p<0,05), mas não para ADM em outras direções. Portanto, pode-se concluir que a LWD é uma intervenção terapêutica eficaz para melhorar a dor, a incapacidade do pescoço e a amplitude de movimento do pescoço, juntamente com exercícios de pescoço em pacientes com dor no pescoço.

INTRODUCTION: Neck pain is a significant cause of disability worldwide, caused by various conditions like underlying diseases, mechanical and neuropathic abnormalities. Longwave Diathermy (LWD) is a therapeutic heating modality used to treat many musculoskeletal conditions. OBJECTIVE: To evaluate the efficacy of LWD on pain, disability, and range of motion (ROM) in neck pain. METHODS AND MATERIALS: The study was conducted after ethical approval from the university and registration in clinical trial registry (PTY/2022/155 & CTRI/2022/06/043033). Thirty patients with neck pain of 20-60 years were randomly allocated into two groups. The control group (n=15) performed simple home-based exercises, while in the experimental group (n=15) longwave diathermy was also given along with home exercises, 3 times/week for 2 weeks. The outcome measures like the Visual analogue scale (VAS), neck disability index (NDI), and Neck range of motion were assessed at baseline, at the end of 2 weeks (post-treatment), and after a followup of 2 weeks. RESULTS AND CONCLUSION: There was a significant improvement in pain, disability, and ROM post-intervention in the control group and experimental group. Additionally, there was a significant difference in pain after follow-up in both groups. The between-group comparison suggested that there was a significant difference for VAS, NDI, and neck extension ROM (p<0.05) but not for ROM in other directions. Therefore, it can be concluded that LWD is an effective therapeutic intervention for improving pain, neck disability, and neck range of motion along with neck exercises in patients with neck pain.

Effects of pre-treatment with metoprolol and diltiazem on cerebral ischemia/reperfusion-induced injuries

Abstract Stroke is one of the most important health concerns worldwide. Calcium ions accumulation in the nerve cells and increase in the catecholamines level of the brain following cerebral ischemia/reperfusion (I/R) are accompanied by damaging effects. Therefore, the present study aimed to evaluate the effects of diltiazem, as a calcium channel blocker, and metoprolol, as a β-adrenoceptors antagonist, on I/R injury. In this study, 30 male Wistar rats were divided into control, I/R, metoprolol, diltiazem, and metoprolol plus diltiazem groups (n=6 in each). Metoprolol (1 mg/kg/day) and diltiazem (5 mg/kg/day) were injected intraperitoneally (i.p.) for 7 days before I/R induction. On day 8, the animals underwent ischemia by bilateral common carotid arteries occlusion for 20 min. Histopathological analysis showed a significant reduction in leukocyte infiltration in diltiazem, metoprolol, and diltiazem plus metoprolol treated rats compared with the I/R group (P<0.05, P<0.01, P<0.01, respectively). In addition, in all treated groups, myeloperoxidase activity and malondialdehyde levels in the brain tissue significantly declined compared with the I/R group (P<0.001). Furthermore, pre-treatment with diltiazem and metoprolol alone or in co-administration remarkably reduced infarct size following I/R (P<0.001). Overall, the results indicate the considerable neuroprotective effects of metoprolol and diltiazem in cerebral I/R.

Genotyping of Listeria monocytogenes isolates by high-resolution melting curve (HRM) analysis of tandem repeat locus

Abstract Listeria monocytogenes is responsible for causing listeriosis, a type of food poisoning with high mortality. This bacterium is mainly transmitted to humans through the consumption of contaminated foods. Detection of L. monocytogenes through molecular methods is crucial for food safety and clinical diagnosis. Present techniques are characterized by low discrimination power and high cost, as well as being time-consuming and taking several days to give the final result. In our study, MLVA-HRM (Multiple-Locus Variable-number tandem repeats Analysis ‒ High-Resolution Melting) was investigated as an alternative method for a fast and precise method for the genotyping of L. monocytogenes isolates. Forty-eight isolates of L. monocytogenes obtained from the microbial bank of Department of Microbiology, Iran University of Medical Sciences, were typed by MLVA-HRM analysis using five Variable Numbers of Tandem Repeat (VNTR) loci. A total of 43 different types were obtained. This research demonstrated the usefulness of the MLVA-HRMA method and its ability to discriminate L. monocytogenes isolates. Since this method is easier and more efficient than existing methods, it can be widely used in food processing plants and diagnostic laboratories as a fast and accurate method.

Diferentes técnicas para colocação de enxerto de extensão caudal em rinoplastia / Different techniques for caudal extension graft placement in rhinoplasty

Resumo Introdução: O enxerto de extensão caudal é geralmente um enxerto de cartilagem que se sobrepõe à margem caudal do septo nasal. Uma combinação do enxerto de extensão caudal e a técnica de tongue-in-groove é usada para estabilizar a base nasal, definir a projeção da ponta e refinar a relação alar-columelar. Objetivo: Apresentar algumas novas modificações na colocação dos enxertos de extensão caudal na rinoplastia. Método: Revisão retrospectiva de um banco de dados prospectivamente coletado de 965 pacientes que se submeteram a rinosseptoplastia de junho de 2011 a julho de 2015. Desses, 457 pacientes necessitaram de enxerto de extensão caudal e foram incluídos no estudo. O seguimento mínimo foi de 13,2 meses, com tempo médio de seguimento de 17,4 meses. Resultados: Na maioria dos casos, a comparação das fotografias antes e após a cirurgia foi satisfatória e apresentou melhora do contorno. Pequenas deformidades foram detectadas em 41 pacientes e 11 pacientes necessitaram de cirurgia de revisão. Conclusão: Com essas modificações, o cirurgião pode usar o enxerto de extensão caudal mesmo em desvios angulares do septo caudal. Vários métodos têm sido propostos para correção do desvio septo-caudal.

Local tissue reaction and histopathological characteristics of three different bulking agents: a rabbit model

ABSTRACT Purpose: We assessed the efficacy and safety of a single injection of three bulking agents over the short- and long-term follow-ups in rabbits. Dermal and preputial matrices were compared with Deflux (DxHA) injection. Material and methods: Twenty-four rabbits were divided into three groups. Group I (n=8) underwent the injection of a lyophilized dermal matrix (LDM) beneath the seromuscular layer of the bladder wall. Rabbits in group II (n=8) were injected with lyophilized preputial matrix (LPM). Rabbits of group III (n=8) were injected with DxHA as the control group. They were followed up for 1 and 6 months after the injection. Subcutaneous injection of all bulking agents was also performed in nude mice. Biopsies were stained with LCA (leukocyte common antibody), CD68, CD31, and CD34. Scanning electron microscopy (SEM) and MTT assay were also performed. Results: Immunohistochemistry staining with CD68 and LCA revealed higher inflammation grade in LDM as compared with LPM and DxHA. Fibrosis grade was also higher in LDM both in short- and long-term follow-ups. However, no significant difference was detected in CD31 and CD34 staining between control and experimental groups. SEM analysis showed that the particle size of LPM was more similar to DxHA. MTT assay revealed that cell proliferation was similar in DxHA, LDM, and LPM. In-vivo assay in nude mice model showed more promising results in LPM as compared with LDM. Conclusion: The long-term results demonstrated that LPM was more similar to Deflux with the least local tissue reaction, inflammation, and fibrosis grade.

A Rare Odontogenic Cyst in an Uncommon Area: Case Report and Review of the Literature / Um cisto odontogênico raro em uma área incomum: Relato de caso e revisão da literatura

Abstract Objective Orthokeratinized odontogenic cyst is a rare developmental odontogenic cyst of the jaws. It is a less aggressive intraosseous cyst identified by an orthokeratinized epithelium. Case Report A 50-year-old male patient with the chief complaint of swelling in the anterior part of his face, and, intraorally, there was diffuse swelling in the palatal cortex. On panoramic radiography, there was a well-defined unilocular radiolucency on the right side of the maxilla and palatal cortical expansion, and thinning of the buccal and palatal cortexes was observed. The histopathological examination revealed a pathologic cyst that was lined by a thick orthokeratinized epithelium. Therefore, the diagnosis was orthokeratinized odontogenic cyst. Conclusion The orthokeratinized odontogenic cyst displays characteristic clinical, histopathological, and biological features that differ significantly from those of keratocystic odontogenic tumor (KCOT), but it has a better prognosis and lower recurrence rate. Thus, other radiolucent lesions of the jaws, including keratocystic odontogenic tumor (KCOT), must be considered in the differential diagnosis.

Resumo Objetivo O cisto odontogênico ortoceratinizado é um raro cisto odontogênico maxilar. É um cisto intraósseo menos agressivo, identificado por um epitélio ortoceratinizado. Relato de caso Um paciente do sexo masculino, de 50 anos de idade, com queixa principal de edema na parte anterior da face, e, intraoralmente, havia edema difuso no córtex palatal. Na radiografia panorâmica, havia uma radioluminescência unilocular bem definida no lado direito da maxila e expansão cortical palatina, e desbastamento dos córtex vestibular e palatino. O exame histopatológico revelou cisto patológico revestido por espesso epitélio ortoceratinizado. Logo, o dignóstico foi de cisto odontogênico ortoceratinizado. Conclusão O cisto odontogênico ortoceratinizado apresenta características clínicas, histopatológicas e biológicas que diferem significativamente das do tumor odontogênico ceratocístico (TOC), mas tem melhor prognóstico e menor taxa de recorrência. Portanto, outras lesões radiolúcidas dos maxilares, incluindo TOC, devem ser consideradas no diagnóstico diferencial.

Evaluation of flexural strength and antibacterial effect of orthodontic acrylic resins containing Galla chinensis extract

ABSTRACT Objective: To evaluate different concentrations of Galla chinensis extract (GCE) added to poly(methyl methacrylate) (PMMA), which is widely used for fabrication of removable orthodontic appliances, regarding the effectiveness of this herbal extract on antimicrobial effect and flexural strength of PMMA. Methods: Acrylic resin samples containing 0.4%, 0.8% and 1.6% GCE were prepared. Flexural strength was investigated via three-point flexural strength test for the 15 acrylic resin blocks of each concentration. Disk diffusion test was used to evaluate antibacterial effects of incorporating the same concentrations of GCE into acrylic resin. All these three groups were compared with the control group, with no added GCE, regarding flexural strength and antibacterial properties. Results: Comparison of flexural strength between the three study groups and the control group showed significant differences between the groups (P=0.018). However, there was no significant difference between the groups containing GCE. There were significant differences in antimicrobial activity between the four groups (P=0.026). Conclusion: Within the limitations of this study, it is suggested that incorporation of GCE into PMMA would be beneficial for antimicrobial activity and flexural strength of PMMA, but further studies on other physical properties and antimicrobial effects on other bacterial strain would be beneficial prior to clinical investigations.

RESUMO Objetivo: Avaliar se diferentes concentrações de extrato de Galla chinensis (EGC) adicionado ao polimetilmetacrilato (PMMA), que é amplamente utilizado para a fabricação de aparelhos ortodônticos removíveis, interferem no efeito antimicrobiano desse extrato e na resistência à flexão do PMMA. Métodos: Foram preparadas amostras de resina acrílica com concentrações de 0,4%, 0,8% e 1,6% de EGC. Para a avaliação da resistência à flexão, utilizou-se o teste de flexão em três pontos para as 15 amostras de resina em cada concentração. O teste de disco-difusão foi utilizado para avaliar os efeitos antibacterianos da incorporação das mesmas concentrações de EGC na resina acrílica. Esses três grupos foram comparados ao grupo controle, sem adição do EGC, em relação à resistência à flexão e quanto às propriedades antimicrobianas. Resultados: As comparações dos três grupos com o grupo controle mostraram diferenças significativas (p=0,018) para a resistência à flexão. Entretanto, não houve diferença significativa entre os grupos contendo EGC. Foram encontradas diferenças significativas na atividade antimicrobiana entre os quatro grupos (p=0,026). Conclusão: Dentro das limitações desse estudo, parece que a incorporação de EGC no PMMA seria benéfica para a atividade antimicrobiana e a resistência à flexão do PMMA. Porém, estudos adicionais sobre outras propriedades físicas e sobre os efeitos antimicrobianos contra diferentes cepas de bactérias seriam interessantes antes de se fazer pesquisas clínicas.

The modified method of luxol fast blue for paraffin-embedded myelin sheath staining / El método modificado de luxol fast blue para la tinción de mielina fijada en parafina

SUMMARY: Histological techniques are the study of animal and human tissues through staining and examining them under a microscope. To demonstrate the axonal degeneration and demyelination in histological studies, the Luxol Fast Blue staining is gold standard techniques. In this study, a new histochemical method based on modified Luxol Fast Blue for the staining of the myelin sheath in sciatic nerve tissues described. The sciatic nerves of rats were removed and then the sciatic nerve was immersed in 10 % formaldehyde for one week and embedded in paraffin block. Next, thin sections (5 µm) were cut, using a microtome and stained with conventional and modified Luxol Fast Blue. Our results showed that a new method of modified Luxol Fast Blue staining can accurately identify the myelin in the sciatic nerve fibers. The current study showed that the Luxol Fast Blue combination with Light Green has a good effect on myelin coloration, and the results of this study are comparable to LFB combination with Sirius red.

RESUMEN: Las técnicas histológicas son el estudio de tejidos animales y humanos mediante tinción y examen bajo un microscopio. Para demostrar la degeneración axonal y la desmielinización en estudios histológicos, la tinción Luxol Fast Blue es una técnica estándar de oro. En este estudio, se describe un nuevo método histoquímico basado en Luxol Fast Blue modificado para la tinción de mielina en los tejidos del nervio ciático. Se seccionaron los nervios ciáticos de ratas y luego el nervio ciático se sumergió en formaldehído al 10 % durante una semana y se fijó en bloque de parafina. Posteriormente, se cortaron secciones delgadas (5 µm) usando un microtomo y se tiñeron con Luxol Fast Blue convencional y modificado. Nuestros resultados mostraron que un nuevo método de tinción Luxol Fast Blue modificado puede identificar con precisión la mielina en las fibras del nervio ciático. El estudio actual mostró que la combinación Luxol Fast Blue con Light Green es un buen efecto sobre la coloración de mielina, y los resultados de este estudio son comparables a la combinación LFB con Sirius red.

The Effect of Propranolol on Sperm Parameters, CatSper 2 Gene and Protein Expression, and Oxidative Stress in Adult Mice

The aim of this study was to investigate the effects of propranolol on sperms, histopathology of testes, andCatSper 2 gene and protein expression in adult mice.18 adult male mice were randomly divided into control, propranolol 1 (receiving 10 mg/kg dose) and propranolol 2 group (receiving 15 mg/kg dose for 35 days). The mean amount of sperm parameters in the propranolol 1 and propranolol 2 groups was significantly lower than the control group (p<0.05). CatSper2 gene and protein expression have significantly decreased in propranolol 1 and propranolol 2 groups compared to the control group (P<0.05). Reduction of CatSper2 gene and protein expression in low dose of propranolol was more severe than high dose. In testicular tissues of the propranolol 1 group, vacuoles and necrosis in the germinal epithelium were observed, and in testicular tissues of propranolol 2 group decrease in the thickness of the germinal epithelium, some vacuoles and necrosis were observed in germinal epithelium as well as congestion in the interstitial space. The mean value of thiol and catalase enzyme in the propranolol 1 and propranolol 2 groups, and the mean value of superoxide dismutase in propranolol 1 group, were significantly different compared to the control group (P<0.05)

Analysis of physicochemical and sensory parameters of wine produced from Carica papaya

Wine is one of the most ancient beverages and it consists of a complex mixture of alcohol, sugars, aldehydes,tannins, pectins, vitamins, minerals, and organic acids formed by the action of Saccharomyces cerevisiae onfruit juices. Papaya (Carica papaya) is a common plant originally from South America and grown mainly inAsian and African countries. In the present study, C. papaya fruit pulp was used for the production of wineunder controlled fermentation using S. cerevisiae. The physicochemical parameters such as pH, yeast cellcount, specific gravity, carotenoid content, and alcohol content were observed during the study. The analysiswas done once every 5 days. Fourier Transform Infrared Spectroscopy analyses of wine and unfermented fruitjuice samples also were carried out. During fermentation, the pH was changed from 5.1 to 4.4. The initial valueof yeast cell count during the fermentation process was 8.5×106 cells/ml, later it attained the maximum valueof 9.53×106 cells per ml on the 5th day. It then gradually decreased to a final value of 2.92×106 cells/ml on the20th day. The specific gravity was initially 1.1573 and it decreased to 1.1483. Carotenoid content was 0.034mg/ml on the first day and it increased to 0.279 mg/ml. Alcohol content of papaya was found to be increasedduring the course of fermentation. It was estimated as 3.01% on the 5th day and it increased to 10.11% on the20th day. The overall point for the sensory analysis was 4.8.

Clinical and Histopathological Correlation of Cutaneous Leishmaniasis in Pir Panjal region of Jammu and Kashmir, India

Introduction: Cutaneous leishmaniasis has a wide distribution, spreading from the Indian subcontinent, across Central and South -Western Asia, to the Mediterranean Basin, the northern half of the African continent, and Central and south America. In India, it is endemic to the Thar Desert of Rajasthan. Some cases have been reported from the north western region of Indo-Gangetic plain. The present study was aimed to see the correlation of clinical features and histopathological findings in clinically diagnosed cases of cutaneous leishmaniasis in Pir Panjal region of Jammu and Kashmir which is nonendemic area. Material and Methods: It was an observational and descriptive study and was conducted over a period of 1 year from June 2016 to May 2017 in Pir Panjal region of Jammu and Kashmir. 62 patients with clinically suspicious lesions of CL were studied with particular reference to different histological patterns. Clinical features were correlated with histological patterns. 56 of these were diagnosed as true clinical cases on the basis of criteria for clinical diagnosis and therapeutic response. There were 37 males and 25 females with an age range of 2 to 78 years. These cases were then subjected to slit skin smear and histopathological examination. Results: Out of 62 suspected cases, 38 (61.2%) were smearpositive and 24 (39%) demonstrated Leishman Donovan (LD) bodies in histological sections. 29 of the remaining cases showed one of the recognizable histological patterns seen in CL, 6 did not reveal any suggestive histology but responded to antimonial compound, and 1 turned out to be a case of Discoid lupus erythematosis. Conclusion: Keeping in view the magnitude of problem and limited resources, clinical features may be reliable for diagnosis but this health problem needs further epidemiological studies to know the vectors and parasite strains

Can your eye glasses lead to future neck pain and disability? Relation of eyeglasses with neck muscle endurance and posture

Background: Use of spectacles is common for people having vision problems. Unifocal and bifocal are usually the commonly used lenses for vision correction. The aim of the present study was to compare the effect of type of lenses in eyeglasses on the neck muscles endurance and neck posture.Methods: A sample of 132 subjects were recruited and divided in three groups according to use of unifocal, bifocal or no glasses. Normal healthy subjects using eyeglasses for a minimum of 2 years and as per selection criteria were assessed for their deep neck flexor endurance (DNF) and craniovertebral angle (CVA).Results: Females had less DNF endurance than males in all groups. DNF endurance was also least reported in subjects using bifocal lenses followed by unifocal and no glasses. These results were similar for both males and females. Bifocal group also had least CVA amongst both sexes.Conclusions: Use of bifocal lenses reduces the DNF endurance and leads to forward head posture over time. These two factors are commonly associated with development of neck pain and disability. Assessment for the type of lens in eyeglasses should be added in diagnosing musculoskeletal problems in neck. Appropriate neck exercises, corrective postures and advise for correct eyeglasses at an early stage could maintain DNF endurance and therefore, prevent faulty neck postures and associated pain. This small check could be a big step towards reducing the financial and psychological burden on the patient and an affordable and less invasive public health solution to neck related problems.