Effect of solvent polarity on extraction yield and antioxidant properties of phytochemicals from bean (Phaseolus vulgaris) seeds

The effect of solvent polarity on extraction yield and antioxidant properties of phytochemical compounds in bean seeds was studied. Seed flour of three varieties of bean was extracted in a series of organic solvents with increasing polarity (n-hexane, petroleum ether, chloroform, ethyl acetate, ethanol, acetone and water). Preliminary screening of phytochemicals showed the presence of tannins, flavonoids, cardiac glycosides, anthocyanins, terpenoids, carotenoids, ascorbic acid and reducing compounds in all extracts. One way analysis of variance (ANOVA) of results showed that extraction yield, phytochemical content and antioxidant properties were significantly influenced (p<0.05) by the polarity of extracting solvents. The regression analysis of data showed polarity-dependent second order polynomial variations in the extraction yield, phytochemical contents, antioxidant activity, reducing properties and free radical scavenging activity of each variety. Extraction in highly polar solvents resulted in high extract yield but low phenolic and flavonoid content as compared to non-polar ones. The polarity-dependent increase in total antioxidant activity and reducing properties indicates the extraction of strong antioxidant compounds in polar solvents. The study suggests the use of a combination of polar and nonpolar solvents to increase the extraction efficiency of phytochemicals with good antioxidant quality from the bean and other legume seeds.

Comparative Evaluation of Physical and Physicochemical Properties and Antioxidant Potential of Various Cooking Oils

The study was based on the comparative evaluation of physical and physicochemical properties and antioxidant potential of different cooking oils as awareness for the consumers. The cooking oils extracted from sunflower, corn, canola, soybean, and rapeseed and available for consumers as different commercial brands were purchased from the local market and analysed for their physical, physicochemical and antioxidant properties. All of the selected oils were found to be statistically similar on the basis of their physical properties including odour, specific gravity (P=.65) and refractive index (P=0.84). All of the selected oils contained vitamin A except one brand of each of the sunflower, corn and canola oils. The selected oils and their blend showed statistically different physicochemical properties and antioxidant potential (P=.000). The corn oil and rapeseed oil were found to be the best quality oil due to comparatively lower acid, peroxide and saponification values and higher antioxidant potential in terms of free radical scavenging capacity. The study results would provide valuable information to the consumers and the researchers regarding the selection of the best quality cooking oils available in the market.

Influence of high-dose gamma radiation and particle size on antioxidant properties of Maize ( Zea mays L. ) flour

ABSTRACT Influence of high-dose gamma radiation and particle size on antioxidant properties of maize (Zea mays L.) flour was studied using response surface methodology. A central composite design based on three levels of each of particle size, in terms of mesh number (40, 60 and 80 meshes), and gamma radiation dose (25, 50 and 75 kGy) was constructed. A statistically significant dose-dependent decrease (p<0.05) in antioxidant properties of gamma irradiated flour was observed. However, an increase in the mesh number (decrease in particle size of flour) resulted in an increase in antioxidant properties. The optimum level of radiation dose to achieve maximum value of responses was found to be 50 kGy for Trolox equivalent total antioxidant activity (TETAOA), 25 kGy for iron chelating ability (ICA), 25 kGy for reducing power (RP) and 75 kGy for linoleic acid reduction capacity (LARC). However, the optimum level of mesh number to achieve desired levels of TETAOA, ICA, RP and LARC was found to be 80 meshes

Cancrum oris

Cancrum oris [noma] is an orofacial gangrene, which causes progressive and mutilating destruction of the infected tissues. The disease mainly occurs in children having malnutrition, poor oral hygiene and debilitating illness. It is well-documented in literature. In the international statistical classification of WHO, it is mentioned as necrotizing ulcerative stomatitis of children. Noma has disappeared from industrialized countries since the 20[th] century, but still common in third world countries especially in Africa

Optic neuritis: a three years retrospective study

The aim of our study was to review our clinical experience with optic neuritis. A retrospective review of the medical records of patients admitted for optic neuritis from January 2000 through December 2002, in Ophthalmology Department of Lady Reading Hospital, Peshawar was undertaken. Out of 28 cases of optic neuritis, 10 [35.7%] were male whereas 18 [64.3%] were female. The mean age was 31.25 years. Eight [28.6%] cases had bilateral and 20 [71.4%] cases had unilateral optic neuritis. Nine [25%] eyes had retrobulbar neuritis, 24 [66.66%] eyes had papillitis and three [8.33%] eyes had neuroretinitis. Visual acuity [VA] at presentation was between no perception of light-hand movement in 11 [30.55%] eyes, counting finger-6/60 in 18 [50%] eyes, 6/36-6/18 in five [13.88%] eyes and between 6/12-6/6 in two [5.55%] eyes. Patients had mean follow-up of 39.67 days. Final unaided VA of eyes with optic neuritis was between no perception of light-hand movement in 5 [13.88%] eyes, counting finger-6/60 in 9 [25%] eyes, 6/36-6/18 in 4 [11.11%] eyes and between 6/12-6/6 in 18 [50%] eyes. Two [5.55%] cases had recurrence of optic neuritis during study period. Young females are more affected then males. Majority cases were unilateral. Presentation is slightly late with marked impairment of vision. Papillitis is common clinical type of presentation. Fifty percent of patients regain good vision [6/6 6/12]

Frequency of ocular complications of leprosy in institutionalized patients in NWFP Pakistan

There is no systemic disease, which so frequently gives rise to disorders of the eye as leprosy does. The study was conducted to determine the prevalence and gravity of ocular complications in institutionalized leprosy patients in NWFP. It is important to provide necessary information to leprosy health workers and general physicians in order to sensitize them to early detection and treatment or referral to appropriate centre. A prospective study of ocular complications of leprosy patients was conducted at the leprosy centre of Lady Reading Hospital Peshawar and the Leprosy Hospital Balakot, district Mansehra. The study included a record of the name, age, sex, type, duration of disease and completion of multi-drug therapy [MDT]. Classification of the patients was done according to Ridley and Jopling 5-group system. Visual acuity was tested by Snellen chart and those patients having a vision of less than 3/60 were labelled as blind. Ocular adnexa were examined by naked eye and lacrimal sac regurgitation test was done. Slit lamp biomicroscopy was done for anterior segment examination and direct ophthalmoscope was used for fundoscopy. The authors studied 143 patients in the above mentioned leprosy centres. Out of these, 59 had lepromatous leprosy, 39 borderline tuberculoid leprosy, 9 tuberculoid leprosy, 33 borderline lepromatous leprosy, and 33 borderline leprosy. The majority of patients came from the northern districts of NWFP, including Malakand division and district Mansehra. The male to female ratio was 4:1. The age of the patients ranged from 14 to 80 years and the duration of the disease ranged from 1 year to 48 years. Ocular complications were found in 73% of the patients. These complications included loss of eyebrows in 57 patients, loss of eyelashes in 37, corneal changes [including opacity, ulceration, and/or anaesthesia] in 44, iridocyclitis in 31, lagophthalmos in 36, ectropion in 13, and chronic dacryocystitis in 3. Of the total of 15 [11%] patients who went blind from ocular complications, 16 eyes did so due to corneal opacities, 6 eyes due to cataract, 5 eyes due to chronic anterior uveitis and one eye due to corneal ulcer, panophthalmitis and phthisis bulbi each. Conclusions: A significant number of leprosy patients [73%] have ocular complications. The frequency of ocular complications increases with the increasing age and duration of disease of the patients