ABSTRACT
The purpose of this in vitro study was to analyse the absorbance of dye material in conventional glass ionomer cement [GIC] by applying various commercially available surface protecting layers on GIC. 90 disc-shaped specimens were made using brass mold measuring 7mm in diameter and 2mm in thickness. 30 specimens were selected for each week testing having 6 groups [n=5]. The groups were: G1 [Control group], G2 [Nail polish coated GIC], G3 [Master bond coated GIC], G4 [Copal varnish coated GIC], G5 [Varnal coated GIC], G6 [Cold mold seal coated GIC]. The specimens of each group were immersed in a separate test tube filled with methylene blue dye, and placed in an incubator [37[degree]C +/- 2[degree]C] for 1 week, 2 weeks and 3 weeks' time. After required time period, the specimens were rinsed under distal water for 1 minute and air dried for 1 hour. Next, the specimens of each group were put into new test tubes containing 1ml absolute alcohol and again stored at [37[degree]C +/- 2[degree]C] for 24 hours. Absorbance were recorded in ultraviolet spectrophotometer. Results were analysed by Student t-test and Pearson's correlation. The results suggest that varnal and copal varnish are effective protecting materials with significant difference [P<0.01] after 3 weeks time. Our results conclude that the application of suitable protecting material may lead to longevity of GIC restorative biomaterial in a complexed oral environment
ABSTRACT
To determine the sorption rate of flowable dental composites by using different photo-activation techniques. Ninety specimens of 3 different dental composites were used for study. 7mm in diameter and 2mm thick disc of each material were prepared in the laboratory by using brass moulds. 30 samples were polymerized each by Quartz Tungsten Halogen [QTH], Light Emitting Diode [LED] and Ultraviolet B [UVB] light curing units. Samples were taken out from mould and placed in clean glass tube, immersed in 2% methylene blue solution and placed in incubator for 24hrs. Absorbance was detected at 590nm by using Visible Spectrophotometer. The significant results were observed by using both Light Emitting Diode and Quartz Tungsten Halogen light units [*P< 0.01]. Ultraviolet B narrowband light found to be inappropriate for polymerization purpose. Similarly, composite Clinpro and Bioseal showed significant results in every photo-activation method [*P<0.01]. Light Emitting Diode units were as efficient in curing resin composite as Conventional Halogen lamps are. Ultraviolet B narrowband light was not appropriate in activation of dental composite while Clinpro and Bioseal are efficient flowable composites
Subject(s)
Ultraviolet Rays , Methylene Blue , Spectrum Analysis , SpectrophotometryABSTRACT
Cisplatin is known by its toxicity by disturbing electrolytes homeostasis. Thus we aimed to find out the role of herbal plant Cichorium intybus on Cisplatin - induced toxicity. 24 male Albino Wistar rats were randomly divided into 4 groups: Group I is termed as untreated control; Group II is Cisplatin control and received 3 mg/kg b.w.; i.p.; Group III received C. intybus ethanolic extract at a dose of 500 mg/kg b.w. orally for 10 consecutive days and Group IV is Cisplatin + C. intybus pretreated group. C. intybus is given 30 minutes prior to Cisplatin. Cisplatin-induced electrolytes disturbances is indicated by increase Intra-erythrocyte sodium content, decreased plasma magnesium, calcium and Intra-erythrocyte Na[+]-K[+]-ATPase which implicates the renal toxicity. At a dose of 500 mg/kg b.w. of C. Intybus pretreatment showed partial counter action on the electrolytes imbalances and Na[+]-K[+]-ATPase activity
ABSTRACT
Cisplatin is one of the most commonly used antineoplastic agents. Free oxygen radicals are known to play a major role in cisplatin induced renal and oxidative stress. Sodium selenite as an exogenous source of selenium is used for endogenous selenoprotein synthesis to scavenge the free radicals. The study was designed to investigate the possible protective role of sodium selenite in cisplatin induced renal stress, by using biochemical approaches. Adult male Albino Wistar rats were randomly divided into four groups. The control group received distilled water; sodium selenite group received only sodium selenite [1 mg / kg]; cisplatin group received only cisplatin [3 mg / kg]; cisplatin+ sodium selenite group received sodium selenite [1 mg / kg] for 5 alternate days before cisplatin [3 mg / kg] administration. The effects of sodium selenite on cisplatin-induced oxidative and renal stress were evaluated by plasma creatinine, urea, malondialdehyde, nitrate; kidney tissue malondialdehyde, superoxide dismutase and catalase activities. Administration of cisplatin induced significant increases in plasma creatinine, urea and nitrate concentrations showing renal stress. Cisplatin also induced oxidative stress, as indicated by increased kidney tissue concentrations of malondialdehyde, and reduced activities of superoxide dismutase and catalase. Furthermore, treatment with cisplatin caused a marked elevation of kidney weight and decreased body weight. Sodium selenite pretreatment markedly reduced elevated plasma creatinine, urea and nitrate levels and counteracted the deleterious effects of cisplatin on oxidative stress markers. These results indicate that the sodium selenite might have a protective effect against cisplatin-induced nephrotoxicity and oxidative stress in rat