ABSTRACT
Objective To investigate the effects of Epigallocatechin gallate(EGCG)on IL-1βinduced MIN6 cells apoptosis. M.Methods The experiment group was divided into control group,IL-1β group,IL-1β+ EGCG low concentration group and IL-1β+EGCG high concentration group.Cell activity was detected by CCK8.Insulin secretion was detected by ELISA.cell apoptosis was detected by flow cytometry.The mitochondrial membrane potential was detected by flow cytometry.ATP content and cell ac-tivity of ROS were detected by colorimetry and chemiluminescence method.Results Compared with normal group,IL-1β group showed much lower cell activity,insulin secretion,cell mitochondrial membrane potential and ATP content,and at the same time IL-1βgroup had significantly higher cell apoptosis and ROS activities.After given EGCG,both low concentration group and high con-centration group had higher cell activity,insulin secretion,cell mitochondrial membrane potential and ATP content,at the same time lower cell apoptosis and ROS activities was showed.And the IL-1β+EGCG high concentration group worked more powerful.Con-clusion EGCG has protective effects on IL-1βinduced MIN6 cells apoptosis.Its mechanism may be related to increasing the content of the ATP and mitochondrial membrane potential and protecting mitochondrial function as well reducing the activity of ROS.
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<p><b>OBJECTIVE</b>To investigate the chemical constituents of the roots of Angelica polymorpha.</p><p><b>METHOD</b>Silica gel column chromatography was employed for the isolation and purification of chemical constituents. The structures were identified on the basis of spectral data and chemical evidence.</p><p><b>RESULT</b>Fourteen compounds were isolated and identified as follows, 5-hydroxy-2-[(angeloyloxy) methyl] furan [3', 2': 6, 7] chromone (1), octacosanoic acid (2), isoimperatorin (3), 3'S-(-)-O-acetylhamaudol (4), bergapten (5), iso-oxypeucedanin (6), beta-sitosterol (7), angeliticin A (8) , saxalin (9), pabulenol (10), noreugenin (11), oxypeucedanin hydrate (12), daucosterol (13), sucrose (14).</p><p><b>CONCLUSION</b>Compound 1 is a new chromone, named polymorchromone A. Compounds 2, 4, 11, 13, 14 were isolated from A. polymorpha for the first time.</p>
Subject(s)
Angelica , Chemistry , Organic Chemicals , Chemistry , Plant Roots , ChemistryABSTRACT
Objective:To study the effects of ICA on HepG2.2.15 cell proliferation, their sensitivity to the lysis by CD3AK effector cell, to investigate the reversal action of ICA on hepatocarcinoma cells from immune escape through Fas/FasL pathway.To provide the theoretical and experimental bases for ICA development.Methods:MTT assay was used to detect cell proliferation and CD3AK cells cytotoxicity activity;flow cytometry assay was used to examine expression of surface molecules and apoptosis rate of HepG2.2.15 cells.Results:When HepG2.2.15 cells line was treated with 50 ?g/ml ICA,a significant reduction of the rate of cell proliferation was observed. Inhibition rate at 48h was 22.04%,and 29.68% at 72h.Kinetic study showed that inhibition of cell proliferation was time dependent (P0.05).ICA could inhibit apoptosis of Jurkat cells induced by HepG2.2.15 cells. In the co-culture system of HepG2.2.15 cell and Jurkat T cell, apoptosis ratio of Jurkat cell was reduced from 46.66% to 18.20% by ICA (P