The study of in vitro culture conditions for induction of human neural stem cells to neuron like cells / 中国医师杂志

Objective To explore the in vitro culture conditions for neural stem cells to differentiate into neuron-like cells.Methods Neural stem cells were induced to differentiate by a medium containing bFGF and heparin through adhesive culture.Immunohistochemistry was used to identify the expressions of NSE and GFAP after differentiation.Results Positive cells for NSE were 47.3%?1.7% on the 7th day after induction.The number of NSE positive cells in the induction group was 3 times as many as that in the control group(P

Cultivation of human bone marrow mesenchymal stem cells and induced differentiation into neuron-like cells / 中华器官移植杂志

Objective To explore a way that induce human bone marrow mesenchymal stem cells to differentiate into neuron-like cells in vitro.Methods Bone marrow mesenchymal stem cells were cultivated in a medium containing serum by using density gradient centrifugation method and whole marrow method and were induced to differentiation by ?mercaptoethanol.Immunohistochemistry were used to identify the type of cells.Results The number of cells was((1.73)?(0.44))?10~(7)/ml by using whole marrow method and ((7.65)?(0.52))?10~(7)/ml by using density gradient centrifugation method at the 10th day cultivation respectively.There was statistically significant difference(P