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Objective To study the treatment of highly active antiretroviral therapy (HAART) in patients with HIV infection and AIDS and the patients′ survival time after HAART treatment. Methods Three thousand and one hundred cases of HIV infection and AIDS patients received HAART in 12 months by monthly flow cytometry instrument MultiSET absolute counting method detecting the CD4+T cell levels, bDNA method (detection range 1.60 log ~ 6.10 log RNA copies/mL) detecting serum HIV-1 viral load under rigorous clinical observation. Results Twelve months after treatment, the CD4+T cell count increased an average of 430 × 106/L (P < 0.01). Eight months after treatment, all viral loads reached their measurable levels (below 1.70 log copies/mL) by decreasing 45 log copies/mL on average. Conclusion HIV/AIDS patients showed treatment extremely significant possitive responses to highly active antiretroviral therapy. The patients′ survival rate after treatment has greatly improved compare to that in previous literature.
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<p><b>OBJECTIVE</b>To study the chemical changes of salvianolic acid B and lithospermic acid of Salvia miltiorrhiza under the conditions of high temperature and high pressure and explore the reaction mechanism.</p><p><b>METHOD</b>S. miltiorrhiza extracts, salvianolic acid B and lithospermic acid were put in the reactor under the conditions of high temperature and high pressure (120 degrees C, 0.2 MPa), and the chemical changes and stability was studied.</p><p><b>RESULT</b>Salvianolic acid A was the primary product in salvianolic acid B and lithospermic acid's conversion process, and lithospermic acid was an intermediate in the conversion process of salvianolic acid B. Compared with salvianolic acid B, lithospermic acid could convert into more salvianolic acid A and fewer other products in the same conditions. Salvianolic acid A was not stable under the conditions of high temperature and high pressure, and could sequentially convert into other small molecules.</p><p><b>CONCLUSION</b>Referring to the chemical conversion of salvianolic acid B and lithospermic acid, a method of large-scale preparation of salvianolic acid A can be developed.</p>
Subject(s)
Benzofurans , Caffeic Acids , Depsides , Hot Temperature , Lactates , Pressure , Salvia miltiorrhiza , ChemistryABSTRACT
Objective To study the expression of EI-1 in myocardium during cerebral ischemia and reper-fusion, and to investigate the mechanism of cerebral cardiac syndrome. Method Two hundred and eight SD rats weighting 220～250 gram, were divided into three groups: sham control group (n=48), cerebral ischemia group (n=80), cerebral ischemia/reperfusion group (n=80). The area of cerebral ischemia, and the concentration of sennn ET-1 and CK-MB, and the content of myocardial ET-1 were determined in 0,6, 12,24,48,72 h after cerebral ischemia and reperfusion, and were analyzed by t-test or F-test. Results Cerebral necrosis area was ob-served at 6 h after cerebral ischemia in cerebral ischemia group, and peaked at 12 h (P>0.05). The concentra-tion of CK-MB increased gradually after cerebral ischmia, peaked at 12 h (P<0.05), and then gradually de-creased. The serum concentration of ET-1 peaked at 6 h and then gradually decreased. The content of ET-1 in my-ocardium began to increase at 6 h after cerebral ischemia, and peaked at 12 h (P<0.05). In cerebral ischemia/reperfusion group, all of cerebral necrosis size, CK-MB concentration and myocardial ET-1 concent paelced at 12 h and then gradually decreased (P<0.05). Change of ET-1 concentration in blood was similar to that in cerebral ischemia group. Compared with cerebral ischemia group, the size of cerebral necrosis reduced obviously at 24 h,48 h,72 h in cerebral ischemia/reperfusion group (P<0.05). The concentration of CK-MB in cerebral ischemia/ reperfusion group was higher than that in cerebral isehemia group (P<0.05). The peak time of myocardial ET-1 was shatened in cerebral ischemia/reperfusion group. The change of serum ET-1 was not different between two groups. Conclusions Large area of cerebral ischemia, might cause myocardial injury. ET-1 is involved in the course of myocardial injury following cerebral ischemia. Though cerebral reperfusion can protect brain,but it make myocardial injury more serious,and ET-1 might participate in this course.