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Objective:Analysis of subtype distribution characteristics of human papillomavirus (HPV) infection, pathological findings of HPV-positive patients operated colposcopy and cervical histopathological examination in Changsha from 2020 to 2022.Methods:Retrospective analysis of HPV infection status of 60 354 patients was treated in the First Affiliated Hospital of Hunan University of Chinese Medicine from Jan. 2020 to Oct. 2022. They were divided into<25 years old group (3 250 cases), 25-34 years old group (19 406 cases), 35-44 years old group (17 297 cases), 45-54 years old group (13 104 cases), 55-64 years old group (4 793 cases) and≥65 years old group (2 504 cases). Cervical exfoliated cells were collected from women and specimen of lesion site were collected from men. HPV genotyping detection used polymerase chain reaction (PCR) and flow fluorescence hybridization, which could detect 27 HPV genotypes. Analyze the distribution of HPV subtypes in patients of different age groups and different treatment departments. Analyze the relationship between the infection of HPV subtypes and the outcome of 224 patients with HPV positive who also underwent colposcopy and cervical histopathology. Pearson correlation test was used for correlation analysis, and two-tailed P<0.05 was statistically significant; Pearson chi-square test or continuous correction chi-square test was used to compare the rates between groups, and two-tailed P<0.05 was statistically significant; Chi-square split test was used to compare multiple sample rates. The standard test was corrected according to the number of groups and then compared. The difference was statistically significant when the two-tailed P value was less than the corrected standard test. Results:The overall positive rate of HPV was 16.4% (9 909/60 354). Among all HPV positive patients, affection of single type HPV accounted for 75.5% (7 479/9 909) and affection of multi-type HPV accounted for 24.5% (2 430/9 909). The top six HPV types with infection rates from high to low were: HPV52 22.8% (2 256/9 909), HPV58 11.1% (1 097/9 909), HPV53 10.5% (1 045/9 909), HPV16 9.0% (890/9 909), HPV61 7.8% (774/9 909) and HPV6 7.6% (750/9 909). The difference of overall infection rate between different age groups was statistically significant (χ 2=536.90, P<0.001). The HPV infection rate was the highest in the<25 years old group, 30.1% (978/3 250), and it is higher than the age groups of 25-34 years old, 15.6% (3 035/19 406), group of 35-44 years old, 14.2% (2 464/17 297), group of 45-54 years old, 16.1% (2 115/13 104), group of 55-64 years old, 19.1% (915/4 793) and group of≥65 years old, 16.1% (402/2 504), with statistical significance( P<0.001 respectively). The most common types of HPV infection were type 6, 16.7% (378/2 266) and type 11, 10.5% (239/2 266) in dermatology clinics. The positive rate of HPV16 accounted for 12/17 in cervical intraepithelial neoplasia (CIN) 3 and cervical cancer cases, it was significantly higher than that in the inflammatory cases, 25.0% (35/140) and CIN1 cases, 23.0% (11/48), the statistical values were χ 2=15.02, P<0.001 and χ 2=12.48, P<0.001, respectively, all of which were statistically significant. Conclusions:HPV infection rate is highest in young people under 25 years old. Low-risk HPV6 and 11 are the main types of skin and venereal diseases. Among the total cases, high-risk HPV52, 58 and 53 are the most common types of infection with women in Changsha. However, high-grade cervical intraepithelial neoplasia and cervical cancer are more closely related to HPV16 infection.
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Objective To investigate the feasibility of transplanting bone marrow stromal cells (BMSCs) in the injured spinal cord for differentiation into functional oligodendrocytes in rats. Methods The ultra-structure of transplanted BMSCs in the injured rat spinal cord was observed at 1,3,5 weeks after transplantation by immuno-electron microscopy and low temperature embedding.The expressions of myelin basic protein (MBP) and proteolipid protein (PLP) in the transplanted BMSCs in the injured spinal cord were observed at 1,3,5 weeks after transplantation by immunofluorescence staining and laser confocal scanningmicroscopy. Results The organelles in the BMSCs migrated into the white matter of the spinal cord anddeveloped at 1 week after transplantation,showing the ultra-structure features of oligodendrocytes.The BMSCs migrating into the white matter of the spinal cord showed mature ultra-structure features of oligodendrocytes and formed myelin-like structures at 3 and 5 weeks after transplantation.The BMSCs migrating into the white matter of the spinal cord expressed the MBP and PLP at one week after transplantation,and continued to express the MBP and PLP at 3 and 5 weeks after transplantation. Conclusion The BMSCs transplanted into the injured spinal cord nay partly differentiate into functional oligodendrocytes.
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Objective To determine the optimal time for intravenous injection of bone marrow mesenchymal stem cells(BMSCs)by observing the effects of inflammation after spinal cord injury on the survival and immigration of BMSCs injected intravenously.Methods Forty SD rats were randomized into 1 non-injury group and 7 spinal cord injury(SCI)groups in which the injured spinal cords were harvested at 0 h, 6 h, 12 h, 24 h, 3d, 5 d, and 7 d respectively.Hematoxylin and eosin(HE)staining was used to e-valuate the histopathologic changes.Myoleperoxidase(MPO)activity was measured to study the duration of polymorphonuclear leukocyte(PMNL)infiltration in the spinal cord after injury.Another 40 SD rats were also randomly divided into 1 non-injury group and 7 transplantation groups in which intravenous injection of BMSCs was performed at 0 h, 6 h, 12 h, 24 h, 3d, 5 d, and 7 d after SCI.The survival counts and immigration distance of BMSCs in the spinal cord in all the groups were measured.Results The PMNL infiltration into the injured spinal cord and the MPO activity started from 6 h after SCI.There were a large number of infiltrating PMNL in the spinal cord and the MPO activity reached its peak value at 24 h.The infiltrating PMNL in the injured spinal cord began to decrease and the MPO activity began to weaken from 3 d.Proliferation of glial cells and formation of empty holes in the spinal cord started from 5 d.The survival counts of BMSCs were smaller but the immigration distance of BMSCs was longer in the 0 h, 6 h, 12 h, and 24 h transplantation groups.The survival counts of BMSCs were larger and the immigration distance of BMSCs was longer in the 3 d transplantation group.The survival counts of BMSCs were smaller but the immigration distance of BMSCs was shorter in the 5 d and 7 d transplantation groups.Conclusion The optimal time for intravenous injection of BMSCs may be 3 days after SCI.
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OBJECTIVE: To investigate the therapeutic mechanism of tetromethylpyrazine and aminoguanidine on diabetic nephropathy. METHODS: Diabetic rats were induced by streptozotocin. They were divided into 5 groups: normal control group (group C), untreated diabetic group (group DM), tetromethylpyrazine treated group (group TMP), aminoguanidine treated group (group AG) and tetromethylpyrazine and aminoguanidine treated group (group TMP+AG). The expression of vascular endothelial growth factor (VEGF) in renal cortex of the rats in each group was observed by immunohistochemical staining after 12 weeks. RESULTS: The expression of VEGF in renal cortex of the rats in group TMP+AG and group C was alike. The expression of VEGF in renal cortex of group TMP and group AG decreased significantly as compared with that of group C, but was still above normal level. CONCLUSION: The therapeutic mechanism of tetromethylpyrazine and aminoguanidine on diabetic nephropathy may be inhibiting the over-expression of VEGF in kidney of diabetic rats.