Hodgkin's disease: cytochemical studies of deoxyribonucleic acid [DNA] and glucose 6-phosphate dehydrogenase [C6PD] after cyclopho-sphamide [endoxan] treatment

Deoxyribonucleic acid [DNA] and glucose 6- phosphate dehydrcgenase [G6PD] enzyme were studied in human normal lymph node and Hodgk in's disease cells. It has been observed that DNA was present in chromatin materials, whereas [G6P D] was present as granules distributed uniformaly in the cytoplasm. Under the effect of endoxan, a decreased activity of DNA and G6PD has been noticed. This could be explained on the basis that alkylation of phosphate groups was the main point of attack on the DNA which result in the breakdown of the DNA molecule

Cytochemistry of ribonucleic acid [RNA] in murine liver cells infected with schistosoma mansoni

The changes in the concentration of ribonucleic acid [RNA] were studied in liver cells of mouse after infestation with the cercariae of Schistosoma mansoni. It was found, that there is a fluctuation in the intensity of basophilia in parenchymal cells depending on the interval of schistosomal infection. Using the methyl green-pyronin stain, it was found that RNA is localized in the nucleolus and cytoplasm of both normal and infected hepatic cells of mouse. Pronnounced changes in the concentration of RNA were observed in hepatic cells after 50, 75, and 100 days from the beginning of schistosomal infestation, These changes include a decrease in the intensity of basophilia in 50 days post infection and its increase in 75 and 100 days of infection respectively