Transvaginal ultrasound in laparoscopic myomectomy for reduction of residual myoma and reduction of fibroid recurrence / 中国内镜杂志

Objective To explore the application of vaginal ultrasound in laparoscopic myomectomy for the reduction of fibroid residue and reduce the recurrence of fibroids value. Methods 120 patients were randomly divided into observation group and control group according to the patient's admission time, all the patients received laparoscopic surgery, while the observation group operation was cooperated with vaginal ultrasound continued to eliminate the remaining uterine fibroids. After operation, all the patients were followed up for 12 months. The operation of the two groups was observed, the remnants of postoperative myoma and the recurrence of myoma at 12 months after the operation were recorded. Results The observation group's treatment time, blood loss, postoperative exhaust time and hospital stay compared with the control group had no significant difference (P > 0.05). One case of hematoma, one case of ureteral injury, two cases of bladder injury and one case of rectal injury occurred in the observation group. One case of hematoma, one case of bladder injury, two cases incision infection and two cases of rectal injury occurred in the control group. Comparing the incidence of intraoperative complications between the two groups show no significant difference (P > 0.05). The average diameter of fibroids in the observation group was significantly smaller than that in the control group (P < 0.05). In the observation group, only 4 patients showed fibroid remnants that were significantly smaller than those in the control group (P < 0.05). One case of recurrence occurred in the observation group at 9 months and 12 months after operation, while in the control group, at 3, 6, 9, and 12 months after the operation 1, 2, 5, and 7 cases recurred, respectively. The patients in the observation group had a significantly lower incidence of recurrence than those in the control group (P < 0.05). Conclusion The use of colposcopy in patients underwent laparoscopic myomectomy to guide the removal of residual fibroids can significantly reduce the residual fibroids, it is worth to clinically promote.

Photochemotherapy with psoralen and ultraviolet A induced apoptosis of NB4 cells and its effects on caspase-8 and caspase-8 protein expressions / 中国中西医结合杂志

<p><b>OBJECTIVE</b>To study the regulatory effects of psoralen (PSO) plus ultraviolet A (UVA), which is PUVA, on cell apoptosis of human leukemia cell line NB4 and signal pathway of cell apoptosis.</p><p><b>METHODS</b>Human leukemia cell line NB4 was cultured in vitro. The NB4 cells were treated with PSO extracted from Chinese medicine psoralea fruits at different concentrations (0, 5, 10, 20 and 40 microL) plus UVA of wave length 360 nm at different irradiation time points (0 and 5 min). The apoptosis ratio was detected by flow cytometry (FCM). The ultrastructure changes were observed using transmission electron microscope (TEM). The expressions of Caspase-8 and Caspase-8 protein were detected by immunocytochemical method (ICC).</p><p><b>RESULTS</b>After treatment of PSO at different concentrations with a 0 and 5-min exposure of UVA, the apoptosis rate of NB4 cells increased dose-and time-dependently, and was up to peak after treatment of PSO at 40 microg/mL with 5-min exposure of UVA. An interaction was shown between the two factors (P <0. 01). There were obvious morphological apoptosis of NB4 cells under TEM after treated with PUVA. The expressions of Caspase-3 and Caspase-8 protein were up-regulated by PSO, UVA, and PUVA, but the effects of PUVA on Caspase-3 protein were stronger than PSO and UVA at 12 h time-dependently (P <0.01).An interaction was shown between the concentration of PSO and time of UVA (P <0.01).</p><p><b>CONCLUSIONS</b>The optimal combination of PUVA was PSO in 40 microg/mL and 5-min exposure of UVA. PUVA could induce the apoptosis of NB4 cells and in vitro activate Caspase-3 and Caspase-8 genes.</p>

Development of chemical constituents and bioactivities of Camptotheca acuminate / 中国药学杂志

OBJECTIVE: To review the chemical constituents from Camptotheca acuminate Decne and report bioactivities, which provides detailed information for further investigations and applications of C. acuminate, a plant resource endemic to China. METHODS: Based on 39 domestic and foreign literatures, the analysis, induction and summarization of chemical constituents and bioactivities of C.acuminate. RESULTS and CONCLUSION: The major chemical components of C. acuminate are alkaloids, ellagic acid, and flavonoids. The crude extract of C. acuminate shows anti-tumor, immunosuppression and anti-virus activities.

Effect of combined application of psoralen and ultraviolet A for inducing NB4 cell apoptosis and its impact on Fas/FasL gene expressions / 中国中西医结合杂志

<p><b>OBJECTIVE</b>To explore the effect of combined application of psoralen (PSO, an extract from psoralea) and ultraviolet A (UVA) for inducing the apoptosis of human leukemic cell line NB4 and its impact on the Fas/FasL gene expressions.</p><p><b>METHODS</b>According to factorial design, changes of apoptosis rate and ultrastructure of NB4 cells, as well as the gene and protein expressions of Fas/FasL were observed after cells were treated with PSO in different concentrations and irradiated by UVA of 360 nm wavelength for different times, using flow cytometry, transmission electron microscopy and quantitative polymerase chain reaction (PCR), and the outcomes were treated with variable analysis.</p><p><b>RESULTS</b>(1) After treatment of PSO in concentration of 10, 20, 40, 80 microg/mL combined with a 5-min exposure of UVA, the NB4 cells apoptosis rate induced were 26.57% +/- 0.42%, 30.67% +/- 0.11%, 34.90% +/- 0.30% and 24.63% +/- 0.38% respectively. The effects were dose- and time-dependent, and an interaction was shown between the two actors. (2) After being treated by PSO plus UVA, obvious ultrastructure changes with apoptosis characteristics were shown in NB4 cell under electron microscope. (3) PSO plus UVA showed up-regulatory effect on gene and protein expressions of Fas, and down-regulatory effect on gene and protein expressions of FasL in a dose- and time-dependent manner, with the interaction between the two actors in altering Fas gene expression, also in altering FasL gene and protein expressions.</p><p><b>CONCLUSION</b>Combined application of PSO and UVA can induce the apoptosis of NB4 cells, and the Fas/FasL system is one of the pathways for apoptosis inducing.</p>

Compound huangdai tablet as induction therapy for 193 patients with acute promyelocytic leukemia / 中华血液学杂志

<p><b>OBJECTIVE</b>To report the results of curative and adverse effects of compound huangdai tablet (CHDT) as induction therapy for 193 patients with acute promyelocytic leukemia (APL).</p><p><b>METHODS</b>CHDT was administered 1.25 g orally three times a day after meal for three days, then the dosage was gradually increased to 7.5 g/d.</p><p><b>RESULTS</b>One hundred and ninety-three patients achieved complete remission (CR), 78.8% of whom in 30 to 60 days with an average time of 44.3 d. No serious infection, bleeding or DIC occurred during the treatment course. The major adverse effects were gastrointestinal symptoms. There was no change in lanine transaminase, urea, creatinine or electrocardiographic QTc interval in 110 APL patients observed before and after the treatment.</p><p><b>CONCLUSION</b>CHDT therapy is a modality of higher CR rate, good safety and tolerance without bone marrow suppression for APL patients.</p>

Effect of psoralen plus longwave UVA inducing HL-60 cells apoptosis / 中国实验血液学杂志

The aim of this study was to investigate the effects of the traditional Chinese medicine psoralen (PSO) plus long wave ultraviolet A (PUVA) on apoptosis in HL-60 leukemia cells and its mechanism. The effect of PUVA on HL-60 cell growth was assayed by MTT method and the changes of ultrastructure of cells were observed by electron microscopy. The apoptosis ratios, changes of mitochondrial membrane potential, expression of Fas and FasL protein and Fas and FasL mRNA were detected by FCM and fluorescent quantitation RT-PCR respectively. The expression of Caspase 8 and Caspase 3 protein were detected by immunocytochemistry (ICC). The results showed that the growth of HL-60 cells were inhibited by PUVA in time-and concentration-dependent manner through inducing cell apoptosis. When the irradiation time of long wave ultraviolet A lasted 15 minutes and the concentration of PSO was 80 microg/ml, the inhibition of HL-60 cell proliferation and apoptosis ratios reached the peak. There were obvious apoptotic ultrastructure changes and decrease of mitochondrial membrane potential in HL-60 cells after treatment with PUVA. The expression of Fas mRAN increased and expression of FasL mRNA decreased after treating with PUVA for 4 hours, and the same results of Fas, FasL expression on protein level were obtained also after treating with PUVA for 24 hours. The expression of caspase 8 and caspase 3 protein enhanced and reached the peak after treating with PUVA for 8 hours. It is concluded that the PUVA can inhibit the growth of HL-60 cells and induce apoptosis of these cells. The possible mechanism is supposed to be up-regulating Fas, down-regulating FasL levels and then activating the levels of caspase 8 and caspase 3. The decreasing of mitochondrial membrane potential may be involved in this process probably.

Progress in studies of mechanism of anti-fibrosis traditional Chinese drugs and effective components / 中国中药杂志

The progress in the research of pharmacological mechanism of anti-fibrosis traditional Chinese drugs and the effective components is summarized. It's demonstrated by pharmacological experiments that anti-fibrosis traditional Chinese drugs can inhibit the cell proliferation, regulate the cytokines and ECM, and intervente in the signal transduction.