ABSTRACT
Objective To explore the diagnostic values of procalcitonin(PCT),C-reactive protein (CRP),white blood cell(WBC),and neutrophil(N%)in bacterial infectious diseases. Methods Selected 272 patients with bacterial infectious diseases as the research object,all the patients were in accordance with diagnostic criteria of infection,and confirmed by laboratory examination and microbial cultures. Results The total sensitivity of bacterial infection was PCT > CRP > N% > WBC,and the specificity was PCT > CRP > WBC > N%. After different bacterial infection,klebsiella pneumonia was larger in view of PCT sensitivity than escherichia coli, followed by staphylococcus aureus,streptococcus pneumonia,and bauman acinetobacter in ranking. In view of CRP sensitivity,streptococcus pneumonia was larger than klebsiella pneumonia,followed by escherichia coli, staphylococcus aureus,and bauman acinetobacter in ranking. In respect of WBC sensitiveness,bauman acineto-bacter was larger than escherichia coli,followed by staphylococcus aureus,streptococcus pneumonia and klebsiella pneumoniae.In respect of N% sensitivity,streptococcus pneumonia was larger than klebsiella pneumonia,followed by bowman acinetobacter,escherichia coli,and staphylococcus aureus in ranking.After bacterial infection,PCT, CRP,WBC and N% were significantly higher than normal control group,and significantly higher than normal refer-ence value. After different bacterial infections,in view of the PCT level,klebsiella pneumonia was higher than streptococcus pneumonia,followed byescherichia coli,staphylococcus aureus,and bauman acinetobacter in rank-ing.In view of CRP level,klebsiella pneumonia was higher than streptococcus pneumonia,followed by escherichia coli,staphylococcal aureus and bowman acinetobacter. In view of WBC level,staphylococcus aureus was higher than bowman acinetobacter,followed by escherichia coli,klebsiella pneumonia and streptococcus pneumoniae. In view of N% level,klebsiella pneumonia was higher than streptococcus pneumonia,followed by bowman acineto-bacter,staphylococcus aureus and escherichia coli. Conclusion In terms of the sensitivity of PCT,CRP,WBC and NEC% to bacterial infection,the sequence is PCT>CRP>N%>WBC.
ABSTRACT
BACKGROUND:Bone marrow mesenchymal stem cels can secrete a variety of factors in the local lesion, and these factors can promote cel proliferation and inhibit cel apoptosis. OBJECTIVE: To observe the curative effect of bone marrow mesenchymal stem cel transplantation on the aging heart of rats and to explore the possible mechanism of action. METHODS:Thirty Sprague-Dawley rats were randomized into three groups: normal blank group, model group and treatment group. Aging models were made in the latter two groups by injection of D-galactose. Rats in the treatment group were given alogeneic bone marrow mesenchymal stem cel injection, once a week, totaly four times. At 1 week after final injection, the heart tissues were sliced into sections to observe the pathological changes using hematoxylin-eosin staining. Western blot assay was used to detect the expression of basic fibroblast growth factor in the heart tissues. Real-time PCR was used to measure the expression of p53 mRNA in the heart tissues. RESULTS AND CONCLUSION:Bone marrow mesenchymal stem cel transplantation could improve the pathological morphology of the aging heart. Compared with the model group, the expression of basic fibroblast growth factor in the heart tissues was significantly higher in the treatment group (P < 0.05), but the mRNA expression of p53 was lower (P < 0.05). It is speculated that bone marrow mesenchymal stem cels can interact with heart cels to secrete basic fibroblast growth factor and reduce p53 mRNA expression, thereby playing a curative effect on the aging heart.
ABSTRACT
Objective To explore the application value of combined detection of serum carcinoembryonic antigen(CEA),carbo-hydrate antigen 1 53(CA1 53),carbohydrate antigen 125(CA125)and carbohydrate antigen 1 99(CA1 99)in the diagnosis of lung canc-er.Methods The direct chemiluminescence assay was used to detect the expression levels of serum CEA,CA1 53,CA125 and CA1 99 in 128 cases of patients with lung cancer,84 cases of patients with lung benign disease and 30 cases of healthy individuals. Results The expression levels of serum CEA,CA1 53,CA125,CA1 99 in the lung cancer group was significantly higher than those in the lung benign disease group and the healthy control group(P <0.05).The sensitivity and specificity of serum CEA was high in the lung cancer group,which was 5 1.56% and 92.1 1%,respectively.The optimized group of combined detection was CEA +CA125+CA1 99 and CEA+CA125,and the sensitivity was 75.00% and 64.84%,respectively.Conclusion Single tumor markers have certain auxiliary value in diagnosing lung cancer.Combined detection could increase diagnostic sensitivity and accuracy for lung cancer,but its specificity may decrease accordingly,so clinical diagnosis should pay attention to whether there is the occurrence of false-positive.The combined detection of serum CEA,CA125 and CA1 99 could improve diagnostic sensitivity of lung cancer.