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Atoh1 gene encodes a helix-loop-helix transcription factor which is involved in the generation and differentiation of mammalian auditory hair cells and supporting cells, and regulation of the proliferation of cochlear cells, therefore plays an important role in the pathogenesis and recovery of sensorineural deafness. This study reviews the progress of the Atoh1 gene in hair cell regeneration, with the aim of providing a reference for the study of hair cell regeneration gene therapy for sensorineural deafness.
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Animals , Humans , Basic Helix-Loop-Helix Transcription Factors/genetics , Hair Cells, Auditory/physiology , Transcription Factors , Hearing Loss, Sensorineural , Cell Differentiation , Deafness , Regeneration/genetics , MammalsABSTRACT
Objective@#To analyze the influencing factors for residual lesions after radiofrequency ablation in elderly patients with liver cancer.@*Methods@#Clinical data of 65 elderly patients with liver cancer treated by radiofrequency ablation in our hospital from January 2014 to December 2016 were retrospectively analyzed.The influencing factors on residual lesions after radiofrequency ablation were analyzed by univariate analysis and multivariate logistic regression.@*Results@#Of 102 lesions in 65 elderly patients with liver cancer, 87 lesions were completely ablated at the first time, and the first complete ablation rate of lesion was 85.29%(87/102). All visible lesions were completely ablated in 56 patients at the first time, and the first complete ablation rate of cases was 86.15%(56/65). Fifteen lesions in 9 patients were incompletely ablated.Univariate analysis showed that gender(χ2=0.740, P=0.390), cirrhosis(χ2=0.745, P=0.388), hepatitis B(χ2=0.057, P=0.812)and type of liver cancer(χ2=0.171, P=0.682)had no significant effect on the residual lesions after radiofrequency ablation, but the number of lesions(χ2=6.694, P=0.010), the lesion size(χ2=14.382, P=0.000)and liver function classification(χ2=5.359, P=0.030)had statistically significant effects on residual lesions after radiofrequency ablation.Further multivariate logistic regression analysis showed that the number of lesions ≥ 3(OR=1.916, 95%CI: 1.326~2.571, P=0.029), the size of lesions≥3 cm(OR=2.362, 95%CI: 2.180~2.923, P=0.000)were risk factors for residual lesions after radiofrequency ablation.@*Conclusions@#Radiofrequency ablation has a better therapeutic effect on liver cancer in elderly patients.The number and size of lesions have a certain impact on the residual lesions after radiofrequency ablation.
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Objective To analyze the influencing factors for residual lesions after radiofrequency ablation in elderly patients with liver cancer.Methods Clinical data of 65 elderly patients with liver cancer treated by radiofrequency ablation in our hospital from January 2014 to December 2016 were retrospectively analyzed.The influencing factors on residual lesions after radiofrequency ablation were analyzed by univariate analysis and multivariate logistic regression.Results Of 102 lesions in 65 elderly patients with liver cancer,87 lesions were completely ablated at the first time,and the first complete ablation rate of lesion was 85.29 ％ (87/102).All visible lesions were completely ablated in 56 patients at the first time,and the first complete ablation rate of cases was 86.15％ (56/65).Fifteen lesions in 9 patients were incompletely ablated.Univariate analysis showed that gender(x2 =0.740,P =0.390),cirrhosis (x2 =0.745,P =0.388),hepatitis B (x2 =0.057,P =0.812) and type of liver cancer(x2=0.171,P =0.682)had no significant effect on the residual lesions after radiofrequency ablation,but the number of lesions(x2 =6.694,P =0.010),the lesion size(x2 =14.382,P =0.000) and liver function classification(x2 =5.359,P =0.030) had statistically significant effects on residual lesions after radiofrequency ablation.Further multivariate logistic regression analysis showed that the number of lesions ≥ 3(OR =1.916,95 ％CI:1.326～2.571,P=0.029),the size of lesions≥3 cm(OR =2.362,95 ％ CI:2.180 ～ 2.923,P =0.000) were risk factors for residual lesions after radiofrequency ablation.Conclusions Radiofrequency ablation has a better therapeutic effect on liver cancer in elderly patients.The number and size of lesions have a certain impact on the residual lesions after radiofrequency ablation.
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Objective To investigate the risk factors of laparoscopic cholecystectomy (LC) in patients with previous gastrectomy.Methods This retrospective study reviewed a database of patients who underwent LC at our hospital during the period of Jan 2010 and May 2015.Results Comparesd with those of no history of gastrectomy the average operation time in patients with previous gastrectomy was longer (t =15.608,P ＜ 0.05) the intraoperative blood loss was higher (t =3.061,P ＜ 0.05),and the operation time of patients with a long interval (＞ 5 years) between gastrectomy and LC was shorter (t =6.405,P ＜ 0.01).The conversion rate did not differ between the two groups (P ＞ 0.05),but the conversion rate significantly reduced after a comprehensive preoperative evaluation procedure (x2 =15.282,P ＜ 0.01).Conclusion LC for benign gallbladder diseases is safe,effective and feasible in patients with a history of gastrectomy,if a comprehensive preoperative evaluation is adopted and in experinced hands.
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OBJECTIVE:To establish a method for simultaneous determination of sodium danshensu,protocatechuic aldehyde,salvianolic acid B and phellodendrine hydrochloride in Shenbai shuxin granules.METHODS:HPLC method was adopted.The separation was performed on Waters sunfire-C18 column with mobile phase consisted of acetonitrile-0.05 ％ trifluoroacetic acid (gradient elution) at the flow rate of 1.0 mL/min.The detection wavelength was set at 288 nm,and the column temperature was 30 ℃.The sample size was 5 μL.RESULTS:The linear ranges of sodium danshensu,protocatechuic aldehyde,salvianolic acid B and phellodendrine hydrochloride were 0.040 01-1.600 46 μg(r=0.999 9),0.013 84-0.553 7 μg(r=0.999 9),0.049 32-1.972 94 μg(r=0.999 6),0.014 46-0.578 6 μg(r=0.999 8).The limits of quantitation were 7.68,2.66,4.74,1.38 ng,and the limits of detection were 1.92,0.66,2.36,0.69 ng,respectively.RSDs of precision,stability and reproducibility tests were all lower than 2％.The recoveries were 98.846％-100.762％ (RSD=0.77％,n=6),96.632％-99.463％ (RSD=0.98％,n=6),98.541％-100.432％ (RSD=0.82 ％,n =6),98.607 ％-101.521％ (RSD =1.11％,n =6),respectively.CONCLUSIONS:The method is simple,accurate and precise.It can be used for 4 components in Shenbai shuxin granules.
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Objective To study the effect of enhanced MK gene expression in hepatic carcinoma cells.Methods The recombinant plasmid pIRES2-EGFP-MK was transfected into SMMC 7721 cells.The mRNA and protein expression levels of MK gene in these cells were determined by real-time PCR,Western blotting and flow cytometry.The intracellular DNR accumulation of these cells was measured by flow cytometry.To investigate the effect of MK gene mediated multidrug resistance,MTT assay was employed to determine the cellular sensitivity of different chemotherapeutic drugs in MK-overexpressed SMMC 7721 cells.Results The mRNA and protein expression levels of MK gene significantly increased after the recombinant plasmid pIRES2-EGFP-MK transfected into SMMC 7721 cells,suggesting that the recombinant plasmid pIRES2-EGFP-MK can enhance the transcription of MK effectively.The DNR accumulation of MK transfected cells decreased significantly (4.06 ± 0.88,P ＜ 0.05),and IC50 of MK transfected cells to ADM/5-FU increased significantly (15 ± 3,27 ± 4,P ＜ 0.05).Conclusions After the recombinant plasmid pIRES2-EGFP-MK transfected into hepatic carcinoma cells,expression of midkine increased,enhancing the resistance of hepatic carcinoma cells to chemotherapeutic drugs.
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OBJECTIVE:To establish a method for simultaneous determination of paeoniflorin,berberine hydrochloride and ammonium glycyrrhizinate in Xiaoer huadu powder.METHODS:HPLC method was adopted.The separation was performed on Waters SunFireTM-C18 column with mobile phase consisted of acetonitrile-0.1％ phosphoric acid (gradient elution) at the flow rate of 1.0 mL/min.The detection wavelength was set at 238 nm,and column temperature was 25 ℃.The sample size was 10 μL.RESULTS:The linear ranges of paeoniflorin,berberine hydrochloride and ammonium glycyrrhizinate were 8.808-88.08 μg/mL(r=0.999 8),1.778-17.78 μg/mL(r=0.999 6),2.533-25.33 μg/mL(r=0.999 9),respectively.LOQ were 4.404,0.889,2.533 μg/mL;LOD were 1.101,0.445,1.267 μg/mL.RSDs of precision,stability and reproducibility tests were all lower than 2％.The recoveries were 95.08％-99.61％ (RSD=1.77％,n =9),96.93％-99.94％ (RSD=0.92％,n=9),98.33％-102.05％ (RSD=1.27％,n=9).CONCLUSIONS:The method is simple,accurate and reproducible,and can be used for simultaneous determination of paeoniflorin,berberine hydrochloride and ammonium glycyrrhizinate in Xiaoer huadu powder.
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Objective To measure the volume of olfaction-related cortex in olfactory dysfunction patients after upper respiratory tract infections via MRI,and to analyze the differences in the volume of olfaction-related cortex.Methods Fifteen olfactory dysfunction patients after upper respiratory tract infections (patient group) and fifteen age-and gender-matched normal volunteers (control group) were enrolled in this study to undergo 1.5 Tesla MR scanning.The volumes of olfaction-related cortex,including entorhinal cortex (EC),perirhinal cortex (PRC) and insular cortex (IC),were drawn and computed with Dr.View software.Olfactory function test was performed with the Sniffin' Sticks method which consisted of three tests:odor threshold (THR),odor discrimination (DIS),odor identification (ID),and their sum score (TDI).Statistical differences in the volumetric measures of bilateral EC,PRC,and IC between patient and control group were analyzed by analysis of covariance (ANCOVA) with age and intracranial volume (ICV) as covariates.Statistical differences in the olfactory function between patient and control group were analyzed by ANCOVA with age as a covariate.Results (1) The EC volume of patient group in the left and right side were (1.5 ± 0.3),(1.6 ± 0.1) cm3,while the control group were (1.7 ± 0.2),(1.8 ± 0.3) cm3 ; The PRC volume of patient group in the left and right side were (1.9 ± 0.4),(1.9 ± 0.3) cm3,and the control group were (2.5 ± 0.8),(2.3 ± 0.7) cm3 ; The IC volume of patient group in the left and right side were (5.2 ± 0.4),(5.8 ± 0.5) cm3,and the control group were (5.8 ± 0.8),(6.7 ± 0.2) cm3.EC,PRC and IC volumes of patient group and control group were measured and the results showed that the olfaction-related cortex volume was decreased in patient group showing significant statistical difference (F =4.913,4.793,7.832,5.574,9.842,7.221,P ＜ 0.05).(2) Olfactory function test of patient group and control group was performed and the results showed that the scores of patient group were lower than that of control group,and the differences were significant (F =54.508,118.774,93.039,53.692,74.139,53.626,91.842,91.696,P ＜ 0.01).Conclusions It is feasible to measure the volumes of olfaction-related cortex with MRI,and the volumes of EC,PRC and IC decreased in olfactory dysfunction patients after upper respiratory tract infections compared with normal people.
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[Objective] To discuss the relative factors of re-operational effect on iatrogenic bile duct injury(IBDI)induced by laparoscopic cholecystecto-my(LC). [Method] Make analysis on the clinical data of 20 cases of LC-induced IBDI, respectively discussing the relative factors of clinical features, opera-tional opportunity and technology in the operation of IBDI. [Result] LC-induced IBDI was influenced by operational opportunity, the treatment in the past operation and operator’s level of operation for the effect. [Conclusion] The repair opportunity and treatment of LC-induced IBDI were the decisive factors influencing patients’prognosis;it’s better to find and repair timely and for the surgeon with rich experience to do the operation.
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ObjectiveTo evaluate effect intervened by the linden relaxation technique (LRT) in the group of long-term naval shipmen's anxiety on surface ship.MethodsSelected naval surface ship unit 1402 shipmen serve as study subjects.140 anxiety shipmen (70 ≥ SAS score ≥ 50) screen out with Zung'self-rating anxiety scale (SAS).The person's group with anxiety mood,but did not meet the diagnostic criteria for anxiety disorders of DSM-Ⅳ.Anxiety shipmen were random divided into the linden relaxation technique group ( n =70) and No-intervention control group ( n =70).In the LRT intervention group:LRT exercises time course was 1 time/ day,and 30 min/time to persist 10 days.The control group without relaxation training.Before and after interventions,anxiety level separately were evaluated with SAS.ResultsBefore intervention,there were not statistically significant between intervention group and control group in SAS anxiety scores,respectively ( ( 54.00 ± 4.43 ) vs (53.91 ± 4.88 )).After intervention,there were statistically significant between intervention group and control group anxiety scores,respectively was ( (40.57 ± 8.98 ) vs ( 53.13 ± 5.51 ) ).The difference before and after intervention,anxiety scoring in intervention group (13.43 ± 7.82 ) was lower than that of the control group (0.79 ± 3.41 ).After following 2 months,SAS scores in the intervention group was more lower (36.76 ± 8.57 ) than the right finish intervention,there was significant different; but there was not significant different belong to the control group (52.34 ± 5.50).ConclusionLinden relaxation technique improves anxiety mood in the naval servicemen.
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Objective To discuss some characteristics of aggressive behavior of junior middle school students addicted to violence online game.Methods From Aug.to Dec.at 2009,6000 volunteers have been investigated with the self-made violence online game survey scale and the Chen's were screen out by CIAS,and in which 71 violence game addicts,and 66 entertainment game addicts.They were divided into two groups matched as age and sex(male 55 and female 11 in every group),average age was 14.36±0.81 years old.66 no-play game students served as control group.The mean value and standard deviation of aggressive behaviors in each group were compared with one way ANOVA with SPSS12.0 version.Results There were significant different inter groups in the behavioral scores expressed in physiological attack((21.59±4.36),(18.10±6.10,(14.92±5.61)),spoken language attack((21.04±3.98),(18.36±6.26),(15.66±5.23)),angry mood((21.37±4.43),(18.72±5.90),(16.56±5.70)),hostility cognition((21.69±5.77),(20.06±7.74),(16.39±6.85))and the total score((85.56±13.71),(75.28±22.36),(63.54±20.32))(F1-4=25.07,17.35,3.25,10.40,21.81);Moat severity attack behaviors were observed in the violence online game addictive group,but less done in other two groups(t1-4=3.48,2.68,2.65,10.27,P<0.01;t 1-5=6.66,5.37,4.48,5.30,22.01,P<0.01);and secondary was found in Entertainment online game addicts(t1-5:3.18,2.69,2.16,3.66,11.74,P<0.01).Conclusion Higher attack behavior scores are found in the violence online game in junior middle students,and entertainment online game also induce attack behavior,but is not so notable.
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Objective To find some risk factors correlated with test anxiety of middle school students,and to find out influencing pathway for test anxiety. Methods 647 middle school students were investigated with Sarason' Test Anxiety Scale (TAS), Achievement Motivation Scale (AMS), Coping Style Scale for School Students ( CSS), Eysenck Personality Questionnaire( EPQ), Egma Minnen av Bardndosnauppforrestran(EMBU) and Family Environment Scale-Chinese Version(FES-CV). Statistics were done with version of SPSS14.0,and data were analyzed by Pearson correlation, multiple linenear stepwise regression and path analysis. Results The rates of test anxiety respectively was mild 25.97% ,moderate 45.65% ,severe 28.38%; there were no significant different between the male and female students anxiety ( 16.71 ± 6.44,17.01 ± 7.02, t = 1. 469, P = 0.334). Test anxiety positively correlated with Achievement motivation, reach motivation of competition, endurance, escape, expos, deny the fantasy,family conflicts,parental punished severely,excessive interference,objective deny,overprotective of father.( r 1-16 :0. 214,0. 135,0. 254,0. 216,0. 308,0.472,0. 492,0. 168,0. 249,0. 537,0. 282,0. 102,0. 238,0. 185,0. 233,0.301,0.273; P 1-16 = 0. 000 ～ 0. 030) , and negatively correlated with Problem-solving, rationalizing interpretation, family cohesion, informative, entertaining, emotional expression, organization, parental warmth and understanding ( r1-9: -0. 121, -0. 134, -0. 178, -0. 215, -0. 221, -0. 101, -0. 298, -0. 136, -0. 168; P 1-9 =0.000 ～0.007). Enter test anxiety regression equation is the reached motivation of competition,emotional expose,organization, psychosis, Neuroticism, parent's warm and understanding , mother's refuse and deny ( t 1-7: 2.496,2.521, -2.687, -2. 150,3.503,2.237,2.259; P1-7 =0.001 ～0.038). Conclusion Test anxiety is commonly find in middle school students. Test anxiety is affected by some paths that are personality,achievement motivation,emotional coping style,family environment and parental education methods.
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Objective: To establish the quality standard for Qiankun Liquid.Methods: Fructus Lycii、Fructus Cnidii、Radix Ginseng in Qiankun Liquid were identified by TLC, and the content of icariin was determined by HPLC.Results: Fructus Lycii、Fructus Cnidii、Radix Ginseng could be identified by TLC. Icariin showed a good linear relationship at a range of 0.126～1.008?g, r =0.9999. Conclusion: The method is accurate and can be used for the quality control of Qiankun Liquid.
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Objective: To establish the method of fingerprint analysis on Flos Carthami by HPLC. Methods: The HPLC method was used, chromatography conditions were ODS column, CHOH 3 0.4%H 3PO 4 solution (50∶50) as mobile phase and 380nm at detective wavelength. Results: The result showed that 9 peaks (RSD≤3%, n=10 ) were common.Conclusion: This method is reliable, simple and provides a reference standard for the quality control of Flos Carthami.