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Objective:To explore the relationship of matrix metalloproteinase 2 (MMP-2) protein expression and microvessel density (MVD) in gastric cancer tissues with clinicopathological characteristics of patients as well as the significances.Methods:The disease-free survival (DFS) time, overall survival (OS) time and other clinicopathological data of 60 patients with gastric cancer after radical resection at Pei Country People's Hospital of Xuzhou from January 2010 to December 2015 were retrospectively analyzed. The MMP-2 protein expression and MVD marked by CD105 in 60 specimens of gastric cancer tissues and corresponding adjacent normal tissues were detected by using immunohistochemistry. The correlation of MMP-2 protein expression with MVD and their relationship with clinicopathological characteristics were analyzed.Results:The positive expression rate of MMP-2 protein in gastric cancer tissues was higher than that in adjacent normal tissues, and the difference was statistically significant [75% (45/60) vs. 17% (10/60), χ2 = 59.668, P < 0.05]. The MVD in gastric cancer tissues was higher than that in adjacent normal tissues, and the difference was statistically significant [32±5 vs. 20±4, t = -2.32, P < 0.05]. The expression of MMP-2 protein in gastric cancer tissues had no relationship with gender, age, the longest tumor diameter, tumor location, OS time, and DFS time (all P < 0.05), while the expression of MMP-2 protein in gastric cancer tissues had statistically significant relationship with the depth of tumor invasion, lymph node metastasis, TNM stage, and histological differentiation (all P < 0.05). There were no statistically significant differences in the expressions of MVD marked by CD105 in gastric cancer tissues of patients with different gender, age and tumor location (all P < 0.05). The expressions of MVD marked by CD105 in gastric cancer tissues had statistical differences in patients with different the longest tumor diameter, depth of tumor invasion, lymph node metastasis, TNM stage, histological differentiation, OS time, and DFS time (all P < 0.05). In gastric cancer tissues, the expression of MMP-2 protein and MVD were positively correlated ( r = 0.198, P = 0.027). Conclusions:The MMP-2 protein expression and MVD in gastric cancer tissues are on the increase, and play key roles in the occurrence and development of gastric cancer. They might cooperatively participate in the malignant progression of gastric cancer, and can be used as poor prognostic factors of gastric cancer.
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Objective To report 1 case of iatrogenic botulinum poisoning misdiagnosed as myasthenia gravis,to discuss the clinical manifestation,distinguishing feature and prognosis of iatrogenic botulinum poisoning combined with literature.Methods We briefly introduced one patient with botulinum toxin poisoning misdiagnosed as myasthenia gravis.A 26-year old female was admitted in June 2017 in Gaochun People's Hospital of Nanjing,who received two injections of botulinum toxin 500 U for a short period of time due to cosmetic needs,then she suffered from fatigue,chest distress,eyelid ptosis and other symptoms.The patient was once misdiagnosed as myasthenia gravis,but the results of neostigmine test and repeated nerve stimulation examination were negative.After careful examination of the body (mandibular swelling) and repeated medical history requiring,the final diagnosis was clarified.After the treatment such as fluid infusion for 10 days,her discomforts were relieved.The literature was retrieved from "US National Library of Medicine National Institutes of Health (Pubmed) "," China National Knowledge Infrastructure (CNKI)" and "Wanfang Medicine Data China Information",a total of 17 articles were included in the analysis,including 30 effective cases.We analyzed the general characteristics,clinical manifestations and prognosis of botulinum toxin injection.Results Of the 30 cases,28 cases (96.7％) were female,2 cases (3.0％) were male,aged from 3 to 80 years old.Most of them were botulinum toxin type A,except 2 cases unknown.The clinical onset began from the injection day to 3 weeks after injection.The duration of the disease lasted from 10 days to 6 months.Most of the prognosis was good after treatment.Conclusion Careful attention should be paid to the serious reaction of botulinum toxin to ensure safe medication use.
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Restless legs syndrome (RLS) is defined as a sleep-wake disorder, with the key feature being an urge to move, which can be classified as two types as primary RLS and secondary RLS. Iron deficiency is a key role of RLS, low levels of serum ferritin is noted in RLS lab tests. Pathophysiology of RLS is complex, genetic variants, anatomical network vulnerability, neurotransmission abnormalities and abnormal dopaminergic neurotransmission may all contribute to its pathogenesis. The mild RLS can choose non-drug therapy, but most of the patients need drug therapy especially the severe cases. The aboved aspects are discussed in this review based on recent literatures.
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Rheumatic diseases such as rheumatoid arthritis and Sjogren′s syndrome can be associated with a wide range of peripheral neuropathy. The diagnosis and treatment are more difficult in rheumatic diseases combined with peripheral neuropathy due to the complex etiology and clinical manifestations. This article reviews the recent progress in the diagnosis and treatment of rheumatic diseases-related peripheral neuropathy.
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As a kind of nervous system disease,migraine is more common in female,and has the clinical characteristics of repeated attacks,it is of great significance with standardized treatment in the control of the attacks.Yong female patients with migraine during pregnancy and lactation stage will face lots of special problems because they must first consider the impact of treatment on the mother and fetus.Generally,non-drug therapy is recommended as a first-line treatment,if it is not sufficiently effective,paracetamol is recommended during the pregnancy or sporadic use of sumatriptan,NSAIDs is not recommended during the first or third trimester of pregnancy.Preventive therapy should only be considered in the most severe cases.This review summarized recent documents of the safety of the most used antimigraine medications during pregnancy and breastfeeding,in order to provide treatment recommendations in clinical practice.