ABSTRACT
We studied the effects of simulated microgravity on mouse oocytes maturation, and analyzed whether the tail-suspended model can be applied to investigate simulated microgravity effects on reproductive processes in female mice. Mouse oocytes were cultured in vitro with microgravity simulated by a rotating wall vessel bioreactor and by tail-suspended model, and the maturation rate of the mouse oocytes in the two models were examined in vivo. The maturation rate of mouse oocytes cultured in simulated microgravity was 8.93%, and that was 72.33% in 1g gravity. In ratio, oocyte maturation rate had no significant difference between the rotational group and control group. Microgravity simulated by the tail-suspended model inhibited mouse oocytes maturation and increased the rate of oocytes abnormity. The maturation rate of tail-suspended mouse oocytes was 14.54%, which was significantly lower than that of control group. Tail-suspended model should be an ideal model to investigate simulated microgravity effects on reproductive processes of female mice.
Subject(s)
Animals , Female , Mice , Cells, Cultured , Hindlimb Suspension , Oocytes , Cell Biology , Physiology , Oogenesis , Physiology , Weightlessness SimulationABSTRACT
Abnormal changes during fat formation are closely related to the prevalence of many diseases. In order to understand the formation mechanism of fat, we used atomic force microscopy (AFM) to characterize the morphology and mechanical properties of porcine preadipocytes during the differentiation. Preadipocytes and adipocytes were different morphologically. The surface roughness of adipocytes was less than preadipocytes by detection of the ultrastructure. The mechanical properties of preadipocytes were changed during differentiation with AFM-based force spectroscopy. Preadipocytes were 20% higher than adipocytes in the adhesion force, stiffness and Young's modulus. Therefore, AFM analysis of membrane changes related to adipocytes formation provided quantitative data in the nanometer level for further studying the formation mechanism of the adipocytes.
Subject(s)
Animals , Adipocytes , Cell Biology , Adipogenesis , Cell Differentiation , Physiology , Cells, Cultured , Microscopy, Atomic Force , SwineABSTRACT
@#Objective To explore the clinical features and therapeutic strategies of neuralgia after subfrontal craniotomy.Methods 132 neurosurgical patients undergoing selective subfrontal craniotomy without the pain of the incision of scalp were involved. The onset, severity, and characters of the neuralgia were recorded. The therapeutic effects of medicine and nerve block on neuralgia were observed.Results Supraorbital neuralgia (9.8%) and superficial temporal neuralgia (3%) occurred 3～4 days and aggravated 4～7 days after subfrontal craniotomy. The headache could be persistent and become more serious intermittently and irradiated to frontal, parietal, temperal, and para-orbital region. Physical examination revealed homolateral tenderness of supraorbital notch and para-arteria temporalis superficialis. Severity of headache improved dramatically after nerve block therapy.Conclusion It is important to identify whether supraorbital neuralgia and superficial temporal neuralgia are onset after subfrontal craniotomy. Nerve block therapy gets excellent clinical result in treating post-craniotomy neuralgia.