ABSTRACT
Objective: To optimize a technology of integration on field processing and processing crude drugs of Polygoni Multiflori Radix (PMR) and to provide the scientific evidence for the integration of PMR. Methods: Orthogonal test was used to optimize the integration technology on primary processing and reprocessing with two major characteristic components (stilbene glucoside and combined anthraquinone). To compare the differences between the integration technology and traditional technology on pharmacological activity by models of constipation and swelling of ear in mice with Various index (small intestinal propulsion rate, first defecation time, fecal water content, swelling degree of ear, and inflammatory factors). Results: The results showed that the integration technology of 50℃, 16 h was better, and there was significant difference in chemical composition and Laxative effect between the integration technology and traditional technology. The anti-inflammatory effect of the integrated process was better than that of the traditional technology. Conclusion: The technology of integration of field processing and processing crude drugs is feasible and the operation is good.
ABSTRACT
Objective: To establish an HPLC-fingerprint method for evaluating the quality of the root of Polygonum multiflorum from different habitats in China. Methods: A separation was performed on an Agilent extend C18 chromatographic column (250 mm × 4.6 mm, 5 μm) with gradient elution. The mobile phase consisted of acetonitrile-formic acid water solution (0.1%). And the mobile phase was at flow rate of 1.0 mL/min. The detection wavelength was at 280 nm. HPLC fingerprint chromatograms of 16 batches of crude material of the roots of P. multiflorum were analyzed. Chemometric methods including principle component analysis (PCA), and clustering analysis (CA) were used through the software of Matlab for common peaks. And the content of 2,3,5,4'-tetrahydroxy stilbene-2-O-β-D-glucoside was determined by HPLC. Results: The similarities of the 16 batches of samples were between 0.936 and 0.998. This showed that the quality of the roots of P. multiflorum from different habitats in China was stable. Eighteen common peaks were identified by HPLC/Q-TOF/MS. The results of FCA and CA were consistent with similarity evaluation. The content of 2,3,5,4'-tetrahydroxy stilbene-2-O-β-D-glucoside was consistent with principle component loading factors. Conclusion: The method is simple reliable, and it can provide a standard and guidance for the quality control of the roots of P. multiflorum.