ABSTRACT
AIM:ToinvestigatetheultrastructuralchangesofisletmicrovascularendothelialcellsinSTZ-in-duced type 1 diabetic mice .METHODS:BALB/c mice were randomly divided into diabetic group and control group .The expression of insulin and platelet-endothelial cell adhesion molecule-1 ( CD31) in islet microvessels was detected by immu-nohistochemical staining .The ultrastructural changes of islet βcells and islet microvessels were observed under transmis-sion electron microscope .RESULTS:Compared with control group , the number of islet βcells, ratio of βcells/αcells, average number of secretory granules in βcells and insulin expression area per islet in diabetic group were significantly de-creased (P<0.01).Besides, diabetic group had fewer microvessels with lower expression of CD 31 (P<0.01).Mito-chondria in islet microvascular endothelial cells and pericytes in diabetic group were swelling .The basement membrane of islet microvessels became thicker in diabetic group ( P<0.01 ) .CONCLUSION: Islet microvascular endothelial cells were impaired in type 1 diabetic mice .
ABSTRACT
Objective To explore the correlation between adipocyte factors(adiponectin and visfatin)and fetus intrauterine growth.Methods Enzyme immunoassay was used to measure the adiponeetin and visfatin levels in maternal and umbilical serum from 14 women with fetal growth restriction (FGR group),14 women with maerosomia(macrosomia group)and 14 normal pregnant women(control group).The correlations of cord serum adiponectin and visfatin with matemal serum adiponectin and visfatin were analyzed.Results (1)Serum visfatin levels in FGR mothers[(41.4±5.5)]μg/L were significantly higher than that in control women[(34.7±4.9)μg/L and macrosomia mothers[(37.3±4.4)μg/L;P<0.01,P<0.05].Serum adiponectin levels in maerosomia mothers [(4.1±1.3)mg/L]were significantly lower than that in control women [(6.6±1.5)mg/L]and FGR mothers[(6.4±1.3)mg/L;P<0.01].(2)Serum visfatinin levels in FGR babies[(58.1±7.6)μg/L] were significantly increased than that in control newborns[(42.6±7.8)μg/L]and macrosomia babies[(48.5±9.1)μg/L;P<0.01,P<0.05].Serum adiponeetin levels in macrosomia babies[(6.5±1.3)mg/L]were significantly decreased than that in control newborns[(7.7±1.5)mg/L]and FGR babies[(7.7±1.0)mg/L;P<0.05,P<0.05].(3)Maternal serum visfatin levels were positively correlated with umbilical serum visfatin levels(r=0.720.P<0.01).Umbilical serum adiponectin levels were higher than that in maternal serum,but there were no relationship between them(r=0.301,P>0.05).Conclusion The changes of visfatin and adiponeetin levels may be related to the oceurrenee of FGR and fetal macrosomia.