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Objective To study the influence of long time exposure to low levels of sodium arsenite on Warburg effect in cultured human epithelial cells (SV-HUC-1) at different times.Methods SV-HUC-1 cells were exposed to 0.5 μmol/L sodium arsenite for 10,20,30 weeks and cells cultured without sodium arsenite for 10,20,30 weeks were regarded as control groupsin vitro.Lactate assay kit and glucose assay kit were used to measure the lactate secretion and glucose consumption levels,and cells mRNA and protein expressions of SCL2A1 and hexokinase2(HK2) were detected using Real-time PCR and Western blotting.Results The levels of lactate secretion [(4.67 ± 0.20),(7.47 ± 0.28),(12.46 + 0.47) mmol/L],glucose consumption [(2.86 ± 0.11),(4.25 ± 0.19),(6.38 ± 0.05) mmol/L] and expression of HK2 protein (1.21 ± 0.06,1.36 ± 0.13,1.60 ± 0.12) increased significantly after treated with 0.5 μmol/L sodium for 10,20,30 weeks compared with those of control groups [(3.04 ± 0.11),(3.90 ± 0.32),(4.77 ± 0.24) mmol/L;(2.17 ± 0.15),(2.48 ± 0.24),(2.71 ± 0.13) mmol/L;1.00 ± 0.00;P ＜ 0.05].Compared with control group,the expressions of SCL2A1 mRNA,HK2 mRNA and SCL2A1 protein in SV-HUC-1 cells treated with sodium arsenite for 10 weeks increased but the difference was not statistically significant (P ＞ 0.05).While the expressions of SCL2A1 mRNA,HK2 mRNA and SCL2A1 protein in SV-HUC-1 cells treated with sodium arsenite for 20 and 30 weeks increased significantly compared to those of control groups (P ＜ 0.05).Conclusion Long-term exposure to low concentrations of sodium arsenite can increase glycolysis in SV-HUC-1 and induce Warburg effect.
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Objective To systematically review the relationship between arsenic exposure through drinking water and human cancer risk.Methods China National Knowledge Infrastructure(CNKI), VIP Database, WanFang Data,China Biology Medicine Disc(CBM),PubMed,Embase and Cochrane Library were searched through computer to find out the relationship between arsenic exposure and tumorigenesis.The search period was from January 1,1997 to January 1,2017.Meta-analysis was performed using RevMan 5.3.The heterogeneity of the literatures that met the inclusion criteria was examined,and analysis was performed by using fixed effect model or random effect model. OR and 95%CI were calculated, the publication bias was evaluated by inverted funnel plot, and sensitivity analysis was performed. Results Totally 14 literatures entered into this study, 13 927 people in arsenic exposure group, 5 720 people in control group. The meta-analysis showed that, 14 literatures were homogeneous through the heterogeneity test, and was analyzed in the fixed effect model with OR (95 % CI) = 1.20 (1.09, 1.33),which suggested that the risk of cancer in the arsenic exposure group was 1.20 times higher than that in control group. Inverted funnel plot was basically symmetrical,literatures publication bias was small;the result was more reliable by sensitivity analysis.Conclusion Arsenic exposure through drinking water may be a risk factor of tumor.
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Objective To study the changes of serum biochemical parameters of rats after subchronic fluoride exposure. Methods Totally 60 Wistar rats were randomly divided into three groups by weight, 20 in each group. Control group drinking distilled water, fluorine groups drinking distilled water containing 60 and 120 mg/L NaF,respectively. After exposed to fluoride for 4 and 12 weeks (n = 10), rats were sacrificed by anesthesia and their serum samples were collected. The biochemical techniques were used to test serum triglyceride (TG), total cholesterol (T-CHO), high density lipoprotein (HDL), low-density lipoprotein cholesterol (LDL), magnesium (Mg2+), alkaline phosphatase (AKP), potassium (K+), phosphate (Pi), and calcium (Ca2+) contents. Results After comparison of serum TG, T-CHO, LDL, Mg2+, K+, Pi, Ca2+levels of rats in the 3 groups at 4 and 12 weeks of fluoride exposure, no statistical significant difference was found (P > 0.05). At 4 and 12 weeks, the HDL levels in fluoride-exposed rats decreased compared to that of the control rats (mmol/L,4 weeks:0.40 ± 0.07,0.22 ± 0.05, 0.16 ± 0.04; 12 weeks: 0.38 ± 0.32, 0.31 ± 0.14, 0.26 ± 0.13), the 4 week of 60, 120 mg/L fluoride groups were significantly lower than the control group (P < 0.05). After exposed to fluoride for 4 and 12 weeks, the serum concentrations of AKP in the three groups were significantly different statistically(King unit/100 ml,4 weeks:16.18 ± 5.03,7.57 ± 5.83,6.01 ± 1.65;12 weeks:8.65 ± 3.71,13.70 ± 9.31,18.57 ± 9.16;F=12.38,4.31,P<0.05).At 4 weeks, fluorine group with 60 and 120 mg/L were significantly lower than that in the control group (P < 0.05). However, AKP levels increased with increased fluoride concentrations in rats exposed to fluoride for 12 weeks. Conclusion Subchronic fluoride exposure could change the serum levels of HDL and AKP in rats.
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Objective The study aimed to explore the reliability and validity of public health practitioners 'job satisfaction scale. Methods A questionnaire survey was conducted with professional and technical personnel in the province engaged in the work of public health of investigation;they were enrolled through the multi?stage cluster random sampling method for establishing the scale 's internal consistency reliability and structur?al validity. Results The Cronbach's alpha coefficient of the total satisfaction scale was 0.900. The Cronbach's alpha coefficients were 0.896, 0.781,and 0.799 for career development and achievement,relationship and career retention,and income and promotion latitudes,respectively. Four principal components were extracted and the results showed that the cumulative contribution rate was 69.72%and the factor analysis results were basically consistent with the theoretical structure. Conclusion The scale is thus considered to be a reliable and effective measurement tool assessing public health practitioners'job satisfaction,as it had good reliability and validity.
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Arsenic is one of the chemical pollutants,which is widely distributed in natural environment.International agency for research on cancer (IARC) has made it clear that arsenic and its compounds are carcinogens;endemic arseniasis has become a public health problem that seriously endangers human health.However,the arsenic metabolic and toxic effects of the species vary widely,failure to replicate a suitable carcinogenic model on animals,and there is no recognized carcinogenic mechanism.The author provide a brief review of arsenic metabolism,arsenic genotoxicity,arsenic-induced epigenetic changes,arsenic-induced changes in cell signaling pathways,arsenic and estrogen receptor expression,and arsenic immunotoxicity,to provide a reference for control and prevention of endemic arseniasis.
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Objective To investigate the satisfaction status of patients in the Community Health Service Center,and to provide the basis data for reform and development of the Community Health Service Center in future. Methods 481 patients and 9 Community Health Service Center in Shenyang were randomly recruited in this study by the cluster sampling survey.Several trained interviewers used questionnaires to collect requisite information.Results In the all community health professionals,including technical level,therapeutic efficacy,preventive health service, service attitude and requirement satiation,the trust status of patients was the highest,the rates of satisfaction and very satisfaction got to 92.8%.The satisfaction status for preventive health service was the lowest,only 67.7%.In analysis on influencing factors,medical insurance of patients was the important factor for their satisfaction status.There were significant differences in technical level (χ2 =22.61 ,P <0.01 ),therapeutic efficacy (χ2 =21 .52,P <0.01 ), preventive health service (χ2 =1 4.35,P <0.05),service attitude (χ2 =22.43,P <0.01 ),requirement satiation (χ2 =22.30,P <0.01 ),the trust status of patients (χ2 =1 7.81 ,P <0.01 ).In addition,age and education degree also were the factors on the satisfaction status of patients.There were significant differences among different age group in therapeutic efficacy (χ2 =33.38,P <0.01 ),preventive health service (χ2 =24.43,P <0.05 ),requirement satiation (χ2 =26.55,P <0.01 ),the trust status of patients (χ2 =22.39,P <0.05 ).There were significant differences among different education degree in therapeutic efficacy (χ2 =1 5.79,P <0.05),service attitude (χ2 =1 4.63,P <0.05)and the trust status of patients (χ2 =1 3.50,P <0.05).Conclusion The satisfaction status of patients for service attitude is the highest and age,education degree and medical insurance of patients are the important factors influencing satisfaction status of patients.
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<p><b>OBJECTIVE</b>To investigate the effect of different concentrations of fluoride on the expression of transforming growth factor-beta1 (TGF-beta1), and explore the mechanism of dental fluorosis.</p><p><b>METHODS</b>40 Wistar rats were randomly divided into three groups, and supplied with low dose F(-)(60 mgxL(-1), 13 rats), high dose F(-)(120 mgxL(-1), 13 rats) and distilled water(control group, 14 rats) respectively. After 10 weeks, hematoxylin-eosin (HE) and immunohistochemistry staining were used to observe the changes of the ameloblasts and the expression of TGF-beta1 in rat incisors.</p><p><b>RESULTS</b>The fluoride groups showed typical symptoms of dental fluorosis. The surfaces of the rat teeth fed with fluoride appeared chalky color and cross striation on the enamel surface. The HE staining showed that the morphous of ameloblast were disarranged and cellular derangement, even appeared vacuolar change. TGF-beta1 were expressed both in ameloblast of the secretory and maturation stage, and also in stellate reticulum and stratum intermedium. The expression of TGF-beta1 in rat's ameloblasts in experiment group was significantly lower than that in the control group (P<0.01), yet low dose group and high dose group still had no significant difference (P>0.05).</p><p><b>CONCLUSION</b>By inhibiting the expression of TGF-beta1 in ameloblast, fluoride interferes the normal signal transduction between epithelial and mesenchymal, and affect the differentiation and development of enamel, so leading to the occurrence of dental fluorosis.</p>
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Animals , Rats , Ameloblasts , Cell Differentiation , Dental Enamel , Fluorides , Fluorosis, Dental , Incisor , Phosphates , Rats, Wistar , Transforming Growth Factor beta1ABSTRACT
This paper introduces the airway care of a patient with motor neuron disease treated with mechanical ventilation for eight years. The airway care for the patient focused on individualized suctioning,measures to prevent atelectasis such as lung hyperinflation,adjustments of ventilator parameters,regular weaning exercise,as well as measures to prevent ventilator-associated pneumonia such as prevention of aspiration,strict disinfection and isolation,wound care of tracheotomy. The ventilator-associated pneumonia was effectively prevented. Effective nursing care for patients with long-term mechanical ventilation can prevent atelectasis and ventilator-associated pneumonia.
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Objective Based on the health standards for arsenic in drinking water recommended by WHO (0.01 mg/L) and adopted by decentralized rural water supply in China (0.05 mg/L), to explore the conditions of arsenic metabolism and levels of methylation in population consuming drinking water with different concentrations of arsenic. Methods Three hundred and forty-eight adults in Shanxi exposed to different concentrations of arsenic(≥0.05 mg/L, ≥0.01-0.05) in arsenic species , TAs , first methylation ratio (FMR) and SMR in lower exposure group and the control group. In higher exposure group, the FMR of adults aged over 60 years was significantly higher than that in groups of 18-39 years old and 40-49 years old (P0.05). Conclusion In higher exposure group, the 2nd reaction of the metabolic pathway is more active in female than that in male, and arsenic methylation capacity of the higher age group arise. No significant effects of gender and age on arsenic methylation capacity is found among population exposed to drinking water with lower level of arsenic.
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0.05), iAs% was much higher and the levels of FMR, SMR and DMA% were significantly lower in skin lesion group compared with the control (P
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Objective To study the state of oxidative stress induced by sodium arsenite (NaAsO2) in SV-HUC-1 cell. Methods MTT assay was used to evaluate the viability of the cells. The level of ROS was detected by staining cells with DCFH-DA. The content of GSH and MDA were measured by DTNB and thiobarbituric acid methods. The activity of SOD was measured by xanthine oxidase method. Results Compared with the control group, the viability cells decreased in all the treated groups (P0.05), the activity of SOD in all the treated groups was significantly decreased (P
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Objective To make sure the psychological condition of patients with liver transplantation,and then make out certain effective nursing methods. Methods Investigated 94 patients with liver transplantation by MSSNS,analyzed the datum. Results The average score of the patients with liver transplantation was higher than the in-patients expected those in the department of psychology,P
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Objective To explore the effects of air pollution on children's nonspecific immune function,and find the sensitive indexes reflecting the earlier damages of human health induced by air pollution.Methods The data on air pollution were provided by Benxi and Shenyang environment protective bureaus. 300 children in grade 1 and grade 6,half for girls and half for boys were selected from area with slight air pollution and area with heavy air pollution in Shenyang and Benxi respectively by cluster sampling method.The contents of SIgA and the activities of bacteriolytic enzyme in saliva of children were measured by radioimmunoassay and agar spread assay respectively.Results The difference of the contents of saliva SIgA was observed in children in grade 1 between area with heavy air pollution(70.60 ?g/ml)and area with slight air pollution(97.77 ?g/ml),P
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0.05). In stratified studies, risk of asthma in individuals with null genotype of GSTM1 is 2.667 times of that with wild genotype after exposure to light air pollution. Risk of asthma in individuals living in heavy air pollution area is 2.125 time of that in light pollution area for all wild genotype of GSTM1 individuals, but without statistical significance. Conclusion It was not found that the relationship between GSTM1, GSTT1 polymorphism and asthma. Synergism of genotype of GSTM1, GSTT1 and air pollution was not also seen in this study.
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Objective To explore the regularity of damage of anti-lipid peroxidation by air pollutants, and to provide experimental basis for revealing the pathogenetic mechanism of air pollutants. Methods 80 Wistar rats weighting 180-200 g were randomly and averagely divided into 4 groups, ie control group, lower dose group, middle dose group and higher dose group, which were exposed to particulate, SO2 and NO2 respectively. The rats in lower dose group, middle dose group and higher dose group were perfused by 1 ml normal saline mixed with 7.5, 15 and 30 mg particulate respectively, while the rats in control group were only perfused with 1 ml normal saline. At the 2nd day after exposure to particulate, the rats in 3 dose groups were exposed to the mixed air of SO2 and NO2. The lower, middle, and higher dose group were exposed to mixed air of SO2 and NO2 at the concentrations as following: 8 and 5 mg/m3, 16 and 10 mg/m3, 32 and 20 mg/m3 respectively while the control group was exposed to fresh air two hour per day, continuously for 7 days. Half of the rats in each dose group were killed at the 1st day after the 7-day exposure to SO2 and NO2 , the rest were killed at the 8th day after the 7-day exposure to SO2 and NO2 . The contents of MDA, the activities of GST and SOD in serum, the activities of SOD and the contents of MDA in BALF were measured. Results At the 1st day after the exposure to SO2 and NO2 , lower activities of SOD and GST, and higher contents of MDA in serum of rats were observed in higher dose group compared with those in control group. At the 8th day after the exposure to SO2 and NO2 , significantly lower SOD activities were still observed in higher dose group compared with those in control group. It revealed that higher concentrations of air pollutants could decrease the activities of anti-oxidase and increase the contents of lipid peroxides in serum of rats. However, the SOD activities in BALF showed no significant differences among different dose groups at different time during the exposure period. Higher contents of MDA in BALF of rats were observed in higher dose group at the first day after the exposure to SO2 and NO2 , and in middle and higher dose group at the 8 th day after the exposure to SO2 and NO2 compared with those in control group (P
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Objective To evaluate the health effects of ambient air pollution on the respiratory systems of school children and screen the risk factors associated with respiratory diseases in order to propose air pollution control measures and to protect susceptible population. Methods The air pollution monitoring data were obtained from the local environmental protection bureau. Six thousand elementary school pupils (grade 1 and grade 6, half girls and half boys) were selected from light and heavy air pollution areas. Respiratory health states were assessed by investigation of the children's respiratory symptoms and diseases using a parent-completed questionnaire. The results were analyzed by non-conditional logistic regression analysis to calculate ORs of respiratory symptoms and diseases and 95% confidence intervals, after adjustment for air pollution levels, age, sex, indoor coal-burning, parents' smoking, ventilation and parents' respiratory disease history. Results TSP in lightly and heavily air-polluted areas were 0.59 mg/m3 and 1.02 mg/m3 respectively (P
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Objective To study the effects of inorganic arsenic on the activities of GS and AChE in the central nervous system of the offspring rats. Methods Wistar rats were exposed to arsenitc through drinking water at doses of 10, 50 and 100 mg/L respectively from gestation day 6 until F1 pups 42 days old. The activities of AChE, GS in rat brain regions such as cortex and hippocampus were separately determined in F1 pups 0, 28, 42 days old. Results On the postnatal day 0, there were not any significant changes in AChE and GS activities in arsenic group rats compared with the control rats. An increase of AChE in 100 mg/L arsenic group rats hippocampus was showed on the postnatal day 28.These changes also appeared on the postnatal day 42. Conclusion Consecutive arsenic exposure from embryo to postnatal may induce the activity changes of GS,AChE in the pups brain,which may cause results in the related neurotransmitter concentration changes.