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Objective: To analyze the level and clinical significance of serum 25-hydroxyvitamin D [25-OH-D] levels in pregnant women with subclinical hypothyroidism [SCH] and gestational diabetes mellitus [GDM]
Methods: One hundred pregnant women of 24-28 weeks' pregnancy with SCH combined GDM were selected into the observation group, and 100 healthy pregnant women were selected into the control group during the corresponding period. Examined the thyroid stimulating hormone [TSH], free thyroxine [FT4], 25-OH-D, serum calcium [Ca2+], fasting plasma glucose [FPG], and glycosylated hemoglobin [HbAIc] levels and thyroid peroxide antibody [TPOAb], Thyroglobulin antibody [TgAb] status. Examine and compare TSH, FT4, Ca2+, FPG, HbAIc, TPOAb, and TgAb at different levels of serum 25-OH-D in the observation group. Compared the 25-OH-D levels and the ratio of different contents of 25-OH-D of TPOAb-positive and TgAb-positive SCH pregnant women
Results: The levels of TSH, FPG and HbAIc in the observation group were significantly higher than those in the control group [P<0.01]. Through comparison of FT4 levels between the two groups, the difference had no statistical significance [P>0.05]. The levels of 25-OH-D and Ca2+ in the observation group was significantly lower than those in the control group, and the difference had statistical significance [P<0.01]. Through comparison of TSH, Ca2+, FPG and HbAIc in groups with different serum 25-OH-D levels, the difference had statistical significance [P<0.01]. The positive rates of TPOAb and TgAb of pregnant women in the observation group were higher than in the control group [P<0.05]. The vitamin D deficiency rate of TPOAb or TgAb positive pregnant women in the observation group was higher than those in the TPOAb or TgAb negative pregnant women, the difference had statistical significance [P < 0.05]
Conclusion: blood glucose level in pregnant women with GDM and SCH increased significantly, blood calcium level decreased significantly. This group of people are more likely to have VitD deficiency. Thyroid stimulating hormone and blood glucose levels in pregnant women are negatively correlated with VitD levels. Therefore, serum 25-OH-D level can be used as an important reference index for gestational diabetes mellitus with subclinical hypothyroidism, and it has great clinical significance to maintain it at a normal level
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Purpose To evaluate three-dimensional strain changes of the left ventricle in patients with type 2 diabetes and poor blood glucose control using real-time three-dimensional speckle tracking imaging (RT3D-STI), and to assess the correlation between three-dimensional strains and hemoglobin A1C (HbA1c). Materials and Methods Sixty-nine patients with type 2 diabetes and forty-two control subjects were recruited in this study. The patients were divided into DM1 group (patients with well blood glucose control, HbA1c0.05). LVM of DM2 group was higher than that in control group (P<0.01), but GAS and GLS of DM2 group were lower than those in control group (P<0.05). LVM of DM2 group was higher than that in DM1 group (P<0.01), but GAS and GLS of DM2 group were lower than those in DM1 group (P<0.01). HbA1c was negative correlated with GAS and GLS (r= - 0.773 and 0.465, P<0.01). Conclusion The reduced GAS and GLS of the patients with type 2 diabetes and poor blood glucose control may indicate damaged myocardial function.
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Objective To investigate the expressions of insulin-like growth factor receptor-1 (IGF-1R) and insulin-like growth factor binding protein-3 (1GFBP-3) and their correlations with clinicopathological parameters in the primary colon cancer,as well as their roles in lymph node metastasis of colon cancer.Methods The expressions of IGF-1R and IGFBP-3 in 78 cases of colon cancer tissues and 78 cases of normal colon mucosa tissues were detected by SP immunohistochemical technology and the correlations between the expressions and the clinical pathological parameters of colon cancer were analyzed.Results The positive rate of IGF-1R in colon cancer (66.7％,52/78) was significantly higher than that in control group (24.4％,19/78),x2 =28.150,P =0.000.The positive rate of IGFBP-3 in colon cancer (73.1％,57/78) was significantly lower than that in control group (89.7％,70/78),x2 =7.158,P =0.007.IGF-1R expression in colon cancer was significantly correlated with the invasion (x2 =5.804,P =0.016),TNM stage (x2 =5.246,P =0.022) and lymph node metastasis (x2 =12.955,P =0.000).IGFBP-3 expression in colon cancer was signi-ficantly correlated with the TNM stage (x2 =7.096,P=0.008),lymph node metastasis (x2 =5.893,P =0.015) and distant metastasis (P =0.003).Both with other factors had no significant correlation (P ＞ 0.05).1GF-1R expression and IGFBP-3 expression showed a negative correlation (r =-0.245,P =0.03).Conclusion The over expression of IGF-1R and low expression of IGFBP-3 are associated with TNM stage and lymph node metastasis in colon cancer.IGF-1R and IGFBP-3 may become new targets of the treatment of colon cancer.
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Totally 177 individuals (87 males and 90 females), who took abdominal computer tomography were enrolled. Anthropometric surrogates such as abdominal height( AH), waist circumference( WC), body mass index(BMI), and waist to height ratio(WHtR) were measured. Correlations between AH, WC, BMI, and WHtR with visceral abdominal fat were analyzed, and receiver operating characteristic curves ( ROC) were prepared for detecting abdominal adiposity at or beyond 80cm2 using lumbar vertebra 4-5 cross-section visceral fat areas as the screening cut-point within gender separately. Youden′s index was used to determine cut-off values of AH, WC, BMI, and WHtR by which to classify excess abdominal fat. 70(80. 5% ) males and 59(65. 9% ) females had abdominal obesity. Compared to other anthropometic indicators, AH presented the highest correlation with visceral adipose fat. The pearson correlation efficiencies were 0. 825 and 0. 732, the cut-off points were 20 cm and 20. 3 cm in male and female respectively. After controlling for age, receiver operating characteristic analysis showed that AH, followed by WHtR, outperformed WC and BMI in identifying central obesity participants. Optimal cut-off values of the physical anthropometric indices to indentify abdominal adiposity were 20 cm(AH), and 91 cm(WC) 25 kg/ m2 (BMI), and 0. 52(WHtR) for men, and 20. 3 cm(AH), 86. 5 cm(WC), 25 kg/ m2(BMI), and 0. 54(WHtR) for women. AH is effective for predicting visceral adiposity and might be considered the best predictor of abdominal obesity at a cut-point of 20cm in Chinese adults.
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Objective To investigate the expression of fibroblast growth factor-2 (FGF-2) and fibroblast growth factor receptor-4 ( FGFR-4 ) in the papillary thyroid carcinomas ( PTC ) and clinical significance . Methods Immunohistochemistry and Western blotting for the expression of FGF-2 and FGFR-4 were performed in 89 cases of PTC and 30 cases of normal thyroid tissues ( NTT) adjacent to the tumors .Results Immunohistochemistry results showed that , FGF-2 and FGFR-4 expressions were high in thyroid carcinoma (P0.05).Analyzed by Western blotting technique ,FGF-2 and FGFR-4 expressions in thyroid carcinoma were significantly higher than that in normal tissue ,with decrease of cancer degree of tissue differentiation and significantly up regulated expression (P<0.05).Expressions of FGF-2 and FGFR-4 were in a positive linear correlation in the disease (rs=0.434,P<0.01).Conclusion The expressions of FGF-2 and FGFR-4 are correlated with papillary thyroid cancer and they participated in the process of invasion and metastasis , both of which have a positive synergistic effect .The degree of malignancy and biological behavior are meaningful and comprehensive indicators ,which provide a theoretical basis for the subsequent experimental studies of cellular and molecular biology .
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<p><b>OBJECTIVE</b>To explore the distribution of the main clinical symptoms and signs of primary biliary cirrhosis (PBC) in a population of individuals with positivity for anti-mitochondrial antibody-M2 (AMA-M2).</p><p><b>METHODS</b>A total of 20 970 persons who participated in routine health examinations at our hospital were tested for presence and level of antinuclear antibodies (ANAs) using an indirect immunofluorescence (IIF) assay and of AMA-M2 by western blotting. The chi-square test was used for statistical analysis.</p><p><b>RESULTS</b>Titers of ANAs more than 1:320 were detected in 1 243 of all the study participants, with 156 of those individuals having detectable AMA-M2.The overall rate ofAMA-M2 positivity was 0.74%, with a significantly higher rate among female subjects (males:0.3% (32/10 550) vs.females:1.2% (124/10 420); x2=55.85, P less than 0.05). Among the AMA-M2-positive population there were 66 cases of abnormal liver function, 58 cases of increased alkaline phosphatase, 72 cases of abnormal findings for routine blood testing, 47 cases of gallbladder disease history, 49 cases of diabetes history, 22 cases of allergy, 75 cases of abdominal discomfort, 38 cases of weakness, 3 cases of jaundice, and 11 cases of pruritus. There were significant differences between the AMA-M2-negative individuals and the AMA-M2-positive individuals.</p><p><b>CONCLUSION</b>Among the general population, individuals with substandard states of health, such as those with abnormal findings in routine blood tests and abnormal liver function, should be screened for AMA-M2. This screening will facilitate early diagnosis of PBC and timely initiation of disease management, improving the patient's life quality of life and prolonging their life.</p>
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Female , Humans , Male , Biomarkers , Blotting, Western , Fluorescent Antibody Technique, Indirect , Liver Cirrhosis, Biliary , Metabolism , Pruritus , Quality of LifeABSTRACT
Objective To explore the value of real‐time three‐dimensional speckle tracking imaging (RT‐3D‐STI) in the early detection of globle and regional left ventricular myocardial systolic function in patients of coronary stenosis without regional wall motion abnormality. Methods 64 coronary stenosis patients without regional wall motion abnormality were divided into 2 groups according to angiography (CAG):without coronary stenosis group( n = 20) and coronary stenosis group ( n = 44), and all the myocardial segments of the patients were divided into 5 groups:without coronary stenosis group normal myocardial segments ( n = 340 ), coronary stenosis group normal myocardial segments ( n = 235 ), mild coronary stenosis myocardial segments( n = 126), moderate coronary stenosis myocardial segments( n =213) and severe coronary stenosis myocardial segments( n =174). Real‐time three‐dimensional full volume of left ventricle was obtained, left ventricular global area strain (GAS)and regional area strain(AS) of 17 myocardial segments, etc, were measured by RT‐3D‐STI respectively. The parameters between the two groups and among the five sub groups were compared, the correlation between GAS and coronary artery Gensini score was also analyzed. Results GAS in coronary stenosis group significantly reduced compared with without coronary stenosis group( P 0 0.5) S.egmental AS in moderate and severe coronary stenosis myocardial segments significantly reduced ( P < 0.05 ), and that in severe coronary stenosis myocardial segments was lower than that in moderate coronary stenosis myocardial segments, the differences were statistically significant ( P < 0.05 ). In addition, GAS was significantly correlated with coronary artery Gensini score( r =0.828, P <0.01). Conclusions RT‐3D‐STI can quantitatively assess the early changes of left ventricular globle and regional myocardial systolic function in patients with coronary stenosis.
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Objective To evaluate the role of spinal neuronal extracellular signal-regulated protein kinases 5/cAMP response element binding protein (ERK5/CREB) signaling pathway in withdrawal responses in morphinedependent rats.Methods Ninety-six adult male Sprague-Dawley rats in which intrathecal catheters were successfully placed,weighing 200-250 g,were randomly divided into 4 groups (n =24 each):normal control group (group A),morphine withdrawal group (group B),dimethyl sulfoxide (DMSO) + morphine withdrawal group (group C) and ERK5 inhibitor BIX02188 + morphine withdrawal group (group D).Morphine dependence (MD) was induced by increasing doses of subcutaneous morphine for 6 days.The initial dose of morphine was 10 mg/kg once a day and was increased by 10 mg/kg once a day from 2nd to 5th days until 50 mg/kg on 6th day in B,C and D groups.Morphine withdrawal response (MW) was induced by intraperitoneal naloxone 4 mg/kg at 4 h after last morphine administration in B,C and D groups.In addition,BIX02188 10 μg and 1％ DMSO 10 μl were injected intrathecally at 1 h before naloxone injection in D and C groups,respectively.MW and morphine withdrawal-induced hyperalgesia were scored.The rats were then sacrificed after hyperalgesia was scored and the spinal cord was removed for determination of CREB and phosphorylated CREB (p-CREB) expression.Results Compared with group A,MW and hyperalgesia scores were significantly increased and the expression of p-CREB was up-regulated in B,C and D groups.Compared with group B,MW and hyperalgesia scores were significantly decreased and the expression of p-CREB was down-regulated in D group,and no significant change was found in group C.Conclusion The spinal neuronal ERK5/CREB signaling pathway is involved in withdrawal responses in morphine-dependent rats.
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Objective To investigate the roles of spinal N-methyl-D-aspartic acid receptor-extracellular signal-regulated protein kinases 5 signaling pathway in naloxone-induced withdrawal response in morphine-dependent rats.Methods Ninety-six adult male SD rats weighting 230-250 g were randomly divided into 4 groups:control group,withdrawal group,DMSO group and MK801 group.Rats were subcutaneously injected with morphine.On day 6,rats were injected with naloxone (intraperitoneal) to precipitate morphine withdrawal syndromes.To identify the function of NMDAR-ERK5 signaling pathway in morphine withdrawal,morphine withdrawal-like behavior test and western blot technique were used in this research.The scores of morphine withdrawal symptom,morphine withdrawal-induced allodynia and the activation of ERK5 in spinal cord were observed after intrathecal injection of MK801.Results There was no withdrawal symptoms and withdrawal-induced allodynia in group A after intraperitoneal injection of naloxone.Compared with group A,withdrawal score (45.2±7.3),score of withdrawal-induced allodynia (14.4±3.7) of group B,withdrawal score (44.7±6.2),score of withdrawal-induced allodynia (13.2±2.7) of group C and withdrawal score (28.3±1.6),score of withdrawal-induced allodynia (5.9± 1.1) of group D were significantly increased (P＜ 0.05).Compared with group C,the total withdrawal score (28.3 ± 1.6),score of withdrawal-induced allodynia (5.9± 1.1) of group D were significantly decreased (P＜0.05).Compared with group A,the expression of spinal p-ERK5 of group B (12848±621) and group C (12579±396) were significantly increased (P＜0.05).Compared with group C,the expression of spinal p-ERK5 of group D (5 123±546) was significantly decreased (P＜0.05).Condusion The signaling pathway of spinal N-methyl-D-aspartic acid receptor-extracellular signal-regulated protein kinases 5 contributes to naloxone-induced withdrawal response in morphine-dependent rats.
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Objective To evaluate the changes in the activity of extracellular signal-regulated kinase 5 (ERK5) in the distal cerebrospinal fluid contacting neurons (CSF-CNs) in the mid-brain of morphine dependent rats.Methods Forty-eight male adult Sprague-Dawley rats weighing 230-270 g,were randomly divided into 2 groups (n =24 each) using a random number table:control group (group A) and morphine dependence group (group B).Morphine dependence was induced by increasing doses of subautaneous morphine for 5 consecutive days.The initial dose of morphine was 10 mg/kg twice a day and was increased by 10 mg/kg everyday until 50 mg/kg on 5th dav.The equal volume of normal saline was injected subcutaneously instead of morphine in group A.On 3rd day after morphine dependence was induced,the distal CSF-CNs in the mid-brain was labeled with 30％ cholera toxin subunit B and horseradish peroxidase compound (CB-HRP) 3 μl injected in the lateral cerebral ventricle in the morning.At 4 h after the last injection of morphine,the segments in which CSF-CNs were located were removed,and CB-HRP positive neurons,phosphor-ERK5 (p-ERK5) positive neurons and CB-HRP/p-ERK5 positive neurons were counted.Results Compared with group A,the number of p-ERK5 and CB-HRP/p-ERK5 positive neurons in the mid-brain was significantly increased (P ＜ 0.05),and no significant change was found in CB-HRP positive neurons in group B (P ＞ 0.05).Conclusion The enhanced activity of ERK5 in the distal CSFCNs in the mid-brain may contribute to the development of morphine dependence in rats.
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Objective To investigate the expressions and significances of SIRT1 and p53 in endometrial carcinoma with diabetes mellitus.Methods Immunohistochemistry was carried out to examine the expressions of SIRT1 and p53 in 39 cases of endometrial carcinoma with diabetes mellitus and 40 cases of endometrial carcinoma without diabetes mellitus and 25 cases of normal endometrium,and the correlations between these indexs were analysed.Results The positive rate of SIRT1 and p53 in experimental group was 64.1％,59.0％ respectively,and 85.0％,52.5％ in control group.The positive rate of SIRT1 in experimental group was lower than that of control group (x2 =4.561,P =0.033).The positive rates of SIRT1 and p53 in control group were higher than that of normal endometrium (x2 =21.462,P＜ 0.001; x2 =6.771,P =0.009).Expression of SIRT1 was positively correlated with p53 (r =0.360,P =0.024).Conclusions Hyperglycemia deregulates SIRT1 in endometrial carcinoma.SIRT1 may promote tumorigenesis by silence of p53.
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Objective To investigate the roles of spinal extracellular signal-regulated protein kinases 5 (ERK5) on morphine withdrawal in rats.Methods Ninety-six male and adult SD rats weighting 230-250 g were randomly divided into saline-naloxone-DMSO group (group A),saline-naloxone-BIX02188 group (group B),morphine-naloxone-DMSO group (group C) and morphine-naloxone-BIX02188 group (group D).To set up morphine dependent model,rats were subcutaneously injected with morphine in the increasing dosage method.On day 6,4 h after the injection of morphine,rats were injected with naloxone (intraperitoneal) to precipitate morphine withdrawal syndrome.The scores of morphine withdrawal symptom and morphine withdrawal-induced allodynia were observed after intrathecal injection of ERK5 inhibitor BIX02188.Result There were not withdrawal symptoms and withdrawal-induced allodynia in group A and B after intraperitoneal injection of naloxone.Compared with group A,teeth chatting (7.5± 1.1),wet dog shacks (4.6± 0.7),jump (5.3± 0.7),abnormal position (8.9± 1.9),diarrhea (7.1 ± 1.6),salivation (2.8±0.6),weight loss (7.9±0.9),total withdrawal score (44.8±5.9),score of withdrawalinduced allodynia (14.6±2.4) of group C and teeth chatting (3.1±0.5),wet dog shacks (1.5±0.4),jump (2.2± 0.5),abnormal position (7.9± 1.6),diarrhea (1.8±0.5),salivation (2.8±0.9),weight loss (3.7±0.6),total withdrawal score (23.1± 1.3) and score of withdrawal-induced allodynia (3.5± 1.1) of group D were significantly increased (P＜0.05).Compared with group C,teeth chatting (3.1±0.5),wet dog shacks (1.5±0.4),jump (2.2±0.5),diarrhea (1.8±0.5),weigbt loss (3.7±0.6) and total withdirawal score (23.1±1.3),score of withdrawal-induced allodynia (3.5±1.1) of group D were significantly decreased (P＜0.05).But there was not significant change in abnoral position (7.9±1.6) and salivation (2.8±0.9).Conclusion Inhibition of the activation of spinal cord ERK5 can significantly alleviate withdrawal symptoms of morphine dependent rats by intrathecal injection BIX02188.
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Objective To evaluate the role of extracellular signal-regulated protein kinase 5 (ERK5) in the spinal cord in withdrawal responses in morphine-dependent rats.Methods Ninety-six adult male SpragueDawley rats in which intrathecal catheters were successfully placed,weighing 200-250 g,were randomly divided into 4 groups (n =24 each) using a random number table:normal saline group (group A),withdrawal group (group B),dimethyl sulfoxide (DMSO) group (group C) and ERK5 inhibitor BIX02188 group (group D).Morphine dependence (MD) was induced by increasing doses of subcutaneous morphine for 6 days.The initial dose of morphine was 10 mg/kg once a day and was increased by 10 mg/kg once a day from the 2nd to 5th days until 50 mg/kg on the 6th day in B,C and D groups.Morphine withdrawal response (MW) was induced by intraperitoneal naloxone 4 mg/kg at 4 h after last morphine administration in B,C and D groups.In addition,BIX02188 and 1％ DMSO 10 μl were injected intrathecally at 1 h before naloxone injection in D and C groups,respectively.MW and morphine withdrawal-induced hypemlgesia were scored.The rats were then sacrificed after hyperalgesia was scored and the spinal cord was removed for determination of ERK5 and phosphorylated ERK5 (p-ERK5) expression.Results Compared with group A,MW and hyperalgesia scores were significantly increased and the expression of pERK5 was up-regulated in B,C and D groups (P ＜ 0.05).Compared with group B,MW and hyperalgesia scores were significantly decreased and the expression of p-ERK5 was down-regulated in D group (P ＜ 0.05),and no significant change was found in group C (P ＞ 0.05).Conclusion ERK5 in the spinal cord is involved in withdrawal responses in morphine-dependent rats.
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Objective To investigate an effective therapy to cure Diabetic Neurogenic Bladder (DNB).Methods By adopting multi-center,random,comparative and single-blind clinical experiments,138 patients with DNB from three centers were divided equally into two groups,namely a control group and a therapy group.Patients in the therapy group were treated with acupoint injection therapy with Astragalus injection,while others were treated by western medical based therapy.After four weeks,the effectiveness was collected.Results After the treatment,the TCM syndrome integral of the treatment group and the control group was (18.47± 1.67) and (23.19±2.82) respectively,both reduced than that before the treatment[(29.25±2.12) and (29.13 ± 1.69) respectively]; the difference between the two groups after treatment was statistically significant (P＜0.05) ; bladder residual urine volume in the treatment group and the control group was (88.47± 16.7) ml and (143.19±28.2)ml respectively,both reduced than that before the therapy [(308.90±22.6)ml and (305.90± 20.8)ml respectively],the difference between the two groups after treatment was statistically significant (P＜0.05) ;The total effective rate was 88.4％ and 72.5％ in the treatment group and the control group respectively,showing statistical difference(P＜0.05).Conclusion It is confirmed that acupoint injection therapy with Astragalus injection is an effective curative method for DNB.
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Objective To investigate the differences of related clinicopathologic features in colorectal cancer (CRC) patients with or without Type 2 Diabetes Mellitus (T2DM).Methods Using case-control studies,retrospective analysis of colorectal cancer of 60 patients with colorectal cancer hospitalization and 32 cases of T2DM were taken.The clinical data,histological grade,tumor size,clinical stage and lymph node metastasis pathology indicators conditions of the hospitalized CRC patients with or without type 2 diabetes were compared.Results The average age of the team with hospitalized CRC patients combined with type 2 diabetes is (64.90 ±8.87),fasting blood-glucose is (8.33 ±4.66) mmol/L.BMI and TG are (25.77 ±3.80) kg/m2 and (1.71 ±0.85) mmol/L which were all higher((54.70 ± 11.62),(5.85 ±0.88) mmol/L,(23.57 ±3.64)kg/m2,(1.33 ± 0.83) mmol/L) than patients without DM.There were significant differences between two groups (t =4.324,t =2.982,t =2.728,t =2.045,respectively,P ＜ 0.05).However,total cholesterol,high density lipoprotein-cholesterol and low density lipoprotein-cholesterol had no significantly differences with those patients without DM (P ＞ 0.05).There were no significant differences of clinicopathologic features between CRC patients with and without DM (P ＞ 0.05).Conclusion There is a certain correlation between CRC and DM,DM increases the risk of colorectal cancer so diabetes patients should be early screened for colorectal,with a view to early detection,treatment and improve the prognosis.
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HIC1 (hypermethylated in cancer 1) encodes a transcriptional repressor,and extensively resides in various kinds of normal tissue.HIC1 gene is located in chromosome 17p13.3,in which loss of heterozygote or super-methylation is frequently found in a variety of human cancers.As a new tumor marker,HIC1 has been confirmed down-regulated in a wide variety of solid cancers because of HIC1 promoter hypermethylation,and may be associated with tumor prognosis.As a tumor suppressor,HIC1 participates in the development of tumor process through various ways,and is involved in cell proliferation,tumour growth,and angiogenesis.Therefore,the abnormal hypermethylation or the loss of expression of HIC1 in tumor cells or abnormal function could be one of the important mechanisms of tumor development,and may become a new potential therapeutic targets for cancer.
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ObjectiveTo investigate the control rate and influence factors of glycolated hemoglobin A1C(HbA1c) of type 2 diabetes in Baoding city community.MethodsA cluster-randomized study was conducted and three communities were selected randomly.The study involved all of people aged 45 years old and above in the three communities.The type 2 diabetic patients diagnosed before,were recorded through the cross section study.Then the questionnaire was finished.All diabetic plasma HbA1c was determined by HPLC method.ResultsEighty-seven patients with HbA1c≤7％ were only 18.6％ in all diabetic patients.As the plasma level of HbA1c increased,occurrences of macroangiopathy and microangiopathy were both increased,by the trend test.Logistic regression analysis showed that therapeutic measures and knowledge about diabetes were main influence factors of achieving the hemoglobin Alc target of ＜7％ in type 2 diabetes.ConclusionThere were low HbA1c control in diabetic patients of Baoding community,and knowing diabetes well and receiving insulin treatment might decrease HbA1c level apparently.
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Objective Toinvestigatetheexpressionof SIRT1anditsassociationwith clinicopathologic features in breast carcinoma with type 2 diabetes mellitus.MethodsThe expression of SIRT1 in 30 breast cancer with type 2 diabetes mellitus,65 samples of breast cancer without diabetes mellitus and 18 samples of corresponding normal breast tissues was investigated using immunohistochemistry.ResultsThe positive rate of SIRT1 in breast cancer tissues was significantly higher than that breast cancer with type 2 diabetes mellitus and normal breast tissue (P ＜0.05). In breast cancer with type 2 diabetes mellitus group.The positive rate of SIRT1 was significantly higher than that normal breast tissue(P ＜0.05).The expression of SIRT1 was positively correlated with the number of lymph node(P =0.011),pTNM tumor stage (P =0.028), p53 (P =0.003) and Her-2 (P =0.031) in breast cancer with type 2 diabetes mellitus group. The expression level of SIRT1 in lymph node-positive group was higher than that in lymph node-negative group (P ＜0.05).The expression level of SIRT1 was in lymph node-positive group was higher than that in lymph node-negative group(P ＜0.05).ConclusionSIRTI was up-regulated in breast cancer with type 2 diabetes mellitus,but its expression was lower than that breast cancer without diabetes mellitus,and was associated with the progression of diabetes mellitus. SIRT1 was positively correlated with lymph node, pTNM tumor stage,p53 and Her-2, SIRT1 may be a novel biological parameter to evaluate the malignant degree of breast carcinoma and to predict prognosis of breast cancer.
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Objective To investigate differences of ctinicopathologic features in breast cancer patients with or without Type 2 Diabetes Mellitus(DM).Methods The general conditions and elinicopathologic features of the hospitalized breast cancer patients with or without type 2 diabetes were analyzed using a case-control study.Results The average age,fasting blood-glucose,BMI and TG were(58.4 ± 7.80),(8.15 ± 2.80)mmol/L,(27.72 ± 3.47)mmol/L and(2.36 ± 1.18)mmol/L in patients with DM,and(51.6 ± 9.90),(5.13 ±0.63)mmol/L,(24.15 ± 4.95)mmol/L and(1.32 ± 0.59)mmol/L in patients without DM.There were significantdifference between the two groups(t =2.968,P =0.004; t =5.757,P ＜ 0.001 ; t =3.235,P =0.002; t =4.330,P ＜0.001,respectively).HDL-C in patients with DM was(1.39 +0.20)mmol/L,which was significantly lower than that of(1.50 ± 0.24)mmol/L in patients without DM(t =2.000,P =0.05).TC and LDL-C was(4.89 ± 1.16)mmol/L and(3.02 ±0.90)mmol/L in patients with DM,which were not significantly different with those of(4.79 ±0.85)mmol/L and(2.97 +0.61)mmol/L in patients without DM(t =0.396,P =0.693,and t =0.255,P =0.800,respectively).More patients were in the menopausal status in breast cancer patients with Type 2 DM compared to the other group(x2 =11.835,P =0.001).The expression of Her-2 was 76.7％(23/30)in breast cancer patients with Type 2 DM,which was significantly higher than that of 50.8％(33/65)in patients without DM(x2 =5.689,P =0.017).Conclusion The average age was higher in breast cancer patients with Type 2 DM and most of them were in their menopausal status,furthermore the higher body mass index and worse prognosis were observed in this group,so the breast cancer patients with diabetes should choose the more reasonable treatment.
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SIRT1 (Sirtuin type 1 ), a member of histone deacetylase, dependents on nicotinamide adenine dinucleotide ( NAD + ). It involves in the covalent modification of histones, participates in tumor development and progression through transcription, translation and post-translational modification and so on. Therefore, the expression of SIRT1 in tumor cells or abnormal function could be one of the important mechanisms of tumor development, and may become a new potential therapeutic target for neoplasms.