ABSTRACT
Objective:To explore the in-depth experience of nursing undergraduates′ management experience and managed experience in open laboratory. Analyze management problems and offer suggestions.Methods:Using qualitative research methods and purposive sampling, from April to May of 2019, 40 nursing undergraduates were selected. Semi-structured interview was adopted to interview them and Colaizzi seven-step method was used to analyze the data.Results:Nine themes were grouped into three major parts including work content and feeling of lab assistant, students′ feelings about the management, their needs and suggestions.Conclusions:Teaching departments should pay attention to the feelings of nursing students, and encourage them to jointly build a simulative clinical environment. According to the needs of students, teachers should improve the effectiveness of practice in open laboratory by setting supporting facilities and establish a post practice evaluation.
ABSTRACT
Objective To investigate the effects of ultrasound irradiating hydroxycamptothecin (HCPT)-loaded microbubbles on apoptosis in human hepatoma SMMC-7721 cells, and to clarify its probable mechanism. Methods The SMMC-7721 cells were randomly divided into six groups as follows: control group, blank liposome microbubbles plus ultrasound, HCPT-loaded microbubbles, HCPT, HCPT plus ultrasound,and HCPT-loaded microbubbles plus ultrasound. Apoptosis was ascertained by Annexin V-Fire/ PI and TUNEL methods. The expression of Bcl-2 and Bax protein was detected by immunocytochemistry staining technique. Results In the group of HCPT-loaded microbubbles plus ultrasound,the apoptosis rate at early term was found to reach (12.18 ± 1.38)% by Annexin V-Fitc/PI assay, brown-colored positive apoptotic cells were observed and the apoptosis rate at advanced term was found to reach (34.25 ± 1.83)% with TUNEL method. The expression of Bcl-2 was found to decrease,the expression of Bax increase,and the ratio of Bcl-2/bax significantly decrease by immunocytochemistry staining technique in the same group. Differences with other groups were significant. Conclusions The method of ultrasound irradiating HCPT-loaded microbubbles can notability induce human hepatoma SMMC-7721 cells apoptosis. The decrease in the ratio of Bcl-2 to Bax might be responsible for cells apoptosis.