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Objective:Through the analysis of the evaluation index system of the major rankings of universities and hospitals, this paper aims to provide a reference for the discipline construction of affiliated hospitals in universities.Methods:This paper comprehensively analyzes and compares the evaluation objectives and indicators of the five major rankings of universities and the three major rankings of hospitals in China and abroad.Results:Each main rankings have its own characteristics that both positive and with possible limitations.Conclusions:Hospital management departments can refer certain indicators in order to identify possible gaps of the hospital discipline development. Also, tailored corresponding measurements for discipline development can be developed in combination with real-needs.
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Objective To demonstrate that enolase and Tuf are direct adhesions of Bifidobacterium longum( B.longum) NCC2705.Methods B.lougum NCC2705 was co-cultured with Caco-2.The change of adhesion was tested by microscopy after the purified proteins(GST-Eno and GST-Tuf) were added into the co-culture, and then statistical analysis was conduc-ted.Caco-2 cells were incubated with B.lougum NCC2705 and pathogenic bacteria (Shigella flexneri 2a or Salmonella). The level ofcytokine LDH and TNF-αfor supernatant was tested after the purified proteins ( GST-Eno and GST-Tuf) were added.Results The number of bacteria adherent to Caco-2 was reduced significantly ( P <0.05 ) after the purified proteins ( GST-Eno/GST-Tuf) were added .While Caco-2 was co-cultured with pathogens , the level of LDH and TNF-αwas significantly increased (P<0.05).When Caco-2 was incubated with B.lougum NCC2705 and pathogenic bacteria , the level of LDH and TNF-αwas significantly reduced (P<0.05), but the level of LDH and TNF-αincreased once more (P<0.05) after the purified protein (GST-Eno/GST-Tuf) was added.Conclusion B.longum NCC2705 can competitive-ly inhibit production of pathogenic bacteria in intestinal epithelial cell adhesion , while the enolization enzyme and Tuf proteins can competitively inhibit long bifidobacteria in intestinal epithelial cell adhesion effect .They are adhesion factors of bifidobacteria .
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Objective To investigate the metabolism and function of the intestinal microbiota from liver cirrhosis patients.Methods Sixteen cases of liver cirrhosis and twenty normal individuals were selected , whose intestinal microbiota metagenomic DNA was extracted , followed by high-throughput Solexa sequencing and the bioinformatics analysis of metabo-lism and function annotation to compare the differences between the patients and normal subjects and find out about the cir -rhosis-related functions .Results The functional diversity was significantly reduced in the intestinal microbiota of cirrhotic patients.At the module or pathway level , the intestinal microbiota of patients showed an enrichment in metabolisms of drugs, essential amino acid , propanoate metabolism and inflammatory reaction , whereas an opposite tendency was observed in the metabolic ability of butyrate , bile acid and cell cycle .Conclusion Under the influence of liver cirrhosis , the growth environment in the intestine is destroyed , causing, the intestinal microbiota the exhibit some compensation to adapt to the changed intestinal micro-environment .