Partial Purification of Cellulase Enzyme from Fusarioum oxysporium by Using Egg White Matrix.

Aim: Protein matrix possesses a capacity to bind organic and inorganic matter. Egg white matrix as an adsorbent was utilized for partial purification of cellulase enzyme in detailed batch study. Methodology: Effect of pH, matrix size, enzyme concentration, contact time and temperature on the binding of cellulase on egg white matrix was studied. Desorption studies was carried with variable pH, sodium chloride and ammonium sulphate system to desorbs the cellulase for the egg white matrix. Results: Equilibrium isotherms for the adsorption of the cellulase enzyme on egg white matrix were well fitted to Freundlich and Langmuir isotherm models. The adsorption process has been found endothermic in nature and thermodynamic parameters, Gibb’s free energy (ΔGº), change in enthalpy (ΔHº) and change in entropy (ΔSº) have been calculated as -13276.21, 2025.47 and 698.64 (KJ mol-1) respectively. Thermodynamic studies revealed that the adsorption process is spontaneous and endothermic in nature. In desorption studies it was concluded that maximum elution (66.8% was obtained for cellulase enzyme with 0.3M (NH4)2SO4 with respect to different pH and sodium chloride solution. Cellulase enzyme obtained after desorption process has maximum purification fold of 4.25 with 60% recovery. Conclusion: The results from the current research suggest that egg white can be used for partial purification of cellulase enzyme with less cost.

Optimization of process parameters for cellulase production from Bacillus sp. JS14 in solid substrate fermentation using response surface methodology

The aim of this work was to isolate the potent bacterial strains for the production of cellulose enzyme. A total 30 bacterial isolates showed positive results for the cellulase production but highest enzyme activity was shown by isolate JS 14. From the morphological and biochemical reactions, the isolate was identified as Bacillus sp. Cellulase production was studied by this strain using response surface methodology (RSM). A central composite design (CCD) quadratic response surface was applied to explicate the parameters that significantly affected cellulase production in solid substrate fermentation (SSF). The wheat bran concentration and incubation period were significant factors. The process parameters optimized with response surface methodology was wheat bran concentration 400 g/L; pH, 6.5; temperature, 400C and incubation period 5 days when inoculum 10 % (1x107 cells/ ml) was used for cellulase production in SSF. Supplementation of lactose and CMC to the wheat bran medium favored the enzyme formation.

Partition of glucose oxidase from Aspergillus niger in aqueous two-phase systems based on salt and polyethylene glycol

The aim of this work was to study the isolation of glucose oxidase (GOx) from Aspergillus niger in aqueous two phase system consisting of PEG 7500 (150g l-1), potassium phosphate (175 g l-1, K2HPO4 +KH2PO4) and glucose (10 gl-1), the enzyme was partitioned in polymer phase. By sequential extraction GOx (69.2 percent) was recovered in polymer phase by 11.8 fold purification, giving a yield of 129U mg protein-¹.