ABSTRACT
<p><b>OBJECTIVE</b>To investigate the chemical constituents of lipid-soluble and water-soluble extracts in Flos Sophorae Carbonisatus.</p><p><b>METHOD</b>The compounds were isolated by means of solvent extraction and column chromatography, and their structures were determined by spectral analysis.</p><p><b>RESULT</b>Two compounds from petroleum ether extract and ten from n-BuOH extract were isolated and identified as sophoradiol (1), beta-sitosterol (2), 3 beta, 22 beta, 24-trihydroxy-olean-12-ene (soyasapogenol B) (3), daucosterol (4), kaikasaponin I (5), quercetin (6), isorhamnetin (7), 2-O-methyl-insitol (8), isorhamnetin-3-O-rutinoside (9), isoquercitrin (10), orobol-7-O-beta-D-glucoside (11), rutin (12), respectively.</p><p><b>CONCLUSION</b>Compound 3, 8-11 were isolated from Flos Sophorae Carbonisatus for the first time. The results could be basic foundation for further study on processing mechanism of Flos Sophorae Carbonisatus.</p>
Subject(s)
Sophora , ChemistryABSTRACT
<p><b>OBJECTIVE</b>To study and establish the optimal technology for the preparation of evodiae juice.</p><p><b>METHOD</b>The contents of evodiamine, rutaecarpine and evodin were simultaneously determined with HPLC, and each yield of the three compounds were chosen as the evaluating indicator. The orthogonal test coupled with the weighted coefficient method were adopted to acquire the optimal technology for the preparation of evodiae juice.</p><p><b>RESULT</b>The study showed that the optimal technology for the preparation of evodiae juice was as follows: decocted three times while the first time was with 12-fold of water socked 30 minutes and decocted 45 minutes, the second time was with 8-fold of water decocted 20 minutes and the third time was with 6-fold of water decocted 20 minutes.</p><p><b>CONCLUSION</b>This method is simple and accurate. The optimal technology is suitable for industry manufacture of evodiae juice.</p>
Subject(s)
Drugs, Chinese Herbal , Evodia , Chemistry , Technology, Pharmaceutical , MethodsABSTRACT
On the basis of studying processing method and quality control standard of Realgar,we did researches on the differences of toxicity and efficacy between new processing method(Washed by Acid)and traditional processing method(Grinding Without Water).The result showed that the efficacy was improved and the toxicity reaction Was lowered after Realgar being processed by new method.
ABSTRACT
AIM: To explore the effects of carbonizing temperature and time on HPLC fingerprints of Flos Sophorae for improving the processing technics of Flos Sophorae carbonized.METHODS: Samples were prepared by an oven for different time and at different temperatures,separately.Then the fingerprints of the samples were determined by HPLC,separately.According to the fingerprints of the crude drug,the differences between the fingerprints of the samples were compared. RESULTS: Heated for 30 min,there was no significant change of the fingerprints of the samples which were heated at 120(?C)-150(?C);there was a little change in the fingerprints when the samples were heated at 170(?C),but the significant change was observed when they were heated at 180(?C)-190(?C).The fingerprints showed the chemical constitutents were almost destroyed over 200(?C).Over 160(?C),the effects on the samples which were heated for 60 min were more obviously than on those which were heated for 30 min. CONCLUSION: According to the change of the fingerprints and in view of the efficient usage of the energy,Flos Sophorae carbonizing should be heated at 185(?C)?2 (?C) and for 30 min.