HLA-antigen association with cancer cervix: prognostic and genetic values

The present study was carried out on 26 patients with cancer cervix of various grades and histological types to investigate the possible association of HLA-A, HLA-B and HLA-DR in sera and paraffin embedded sections prepared from tumor tissue. Significant increase in frequency of HLA-B7, B14 and HLA-DR7 was detected in patient's sera compared to those of controls. HLA-DR antigen was expressed in tumor tissue in majority of patients. The intensity of immune expression varied inversely with the survival of patients and varied inversely with the degree of tumor differentiation and proportionally with the severity of associating immuno-competent cellular infiltrate. Despite the relatively little number of studied patient our data confirms an immuno-genetic base in susceptibility to the disease and points to HLA-antigen expression in tumor tissue as a negatively influencing factor, not to be forgotten during evaluating the prognosis of patients with cancer cervix

Oral lichen planus and its association with diabetes mellitus [histopathological and histochemical study]

The present study was carried out on 50 oral Lichen planus patients in an attempt to throw light on the interesting association of oral lichen planus and diabetes mellitus.They were subjected to: 1. Clinical examination 2. Glucose tolerance test 3. Histopathological study 4. Histochemical study of glycogen and succinic dehydrogenase enzyme. According to glucose tolerance test 64% of studied patients had abnormalities in glucose metabolism, with diabetes mellitus was diagnosed in 28% of them.The incidence of diabetes mellitus in patients with oral lichen planus showed predilection towards males and old age. It was directly proportional to remission and exacerbation of the lesions. The histopathological difference between diabetic, potentially diabetic and non- diabetic oral lichen planus lesions was found to be non specific where only the epithelial atrophy and ulceration were found to be more common in diabetic cases while signs of dysplasia were found to be more common in non- diabetic cases. Histochemically, there was decreased glycogen content of tissues involved by oral lichen planus lesions with the degree of glycogen reduction was found to be more marked in diabetic than in non diabetic cases As regard succinic dehydrogenase, it showed decreased activity within the epithelium, especially in the basal cell layer, of oral lichen planus lesions and the degree of enzyme reduction was found to be the same among diabetic, potentially diabetic and non-diabetic lesions.From this study, we concluded that there is an association between oral lichen planus and diabetes mellitus without significant histopthological difference between the lesions of diabetic and non-diabetic patients

Influcence of low energy laser treatment on antigen induced experimental arthritis

This study was performed to evaluate the effect of low energy laser irradiation on arthritis induced by type II collagen in rats 10 rats were used as normal control and 50 were utilized for induction of arthritis, 10 of them were not irradiated [arthritic control group] 20 were subjected to local laser application [experimental group] and 20 were exposed to sham laser irradiation [sham laser irradiated group]. Animals of arthritic control group were sacrificed 2 weeks after induction of arthritis Animal of experimental and sham laser irradiation group were subjected to clinical and radiographic examination before and after irradiation. Then they were sacrificed to evaluated histopathological changes in ankle joints after laser application, compared to arthritic control group. Our data reveal improvement of clinical radiographic and histopathological changes after laser therapy

Cytopathological significance of eosinophilic pleural effusion

388 cases of pleural effusions were examined and out of them 21 eosinophilic effusions were detected. Malignant lesions were present in 9 out of the all 21 detected cases constituted [42.85%] while, non neoplastic lesion were present in the remaining 12 cases. These were heart failure [4 cases], bronchial asthma [one case] SLE [one case] and 6 cases were idiopathic

Correlaton between estrogen receptors and histo-pathological parameters in human breast cancer

A monoclonal antibodies immunoassay technique was used to demonstrate oestrogen receptors in 50 in freshly excised human breast tumours. 48 cases were infiltrating duct carcinoma and 2 cases were infiltrating lobular carcinoma. The relationship between oestrogen receptors, histopathological type, histopathologicalIy and nuclear grades, fibrosis, elastosis and necrosis was evaluated. Oestrogen receptors positivity was demonstrated in 44 out of 50 cases. A significant positive correlation was found between histopathological and nuclear grades and oestrogen receptors content, well differentiated tumours rarely lacked oestrogen receptors. Tumours with marked fibrosis showed less oestrogen receptors content than tumours with mild fibrosis, Elastotic tumours were more oestrogen receptors positive than non elastotic tumours. Those with marked elastosis showed higher oestrogen receptors content than tumours with moderate and mild elastosis. A significant correlation was found between oestrogen receptors content and necrosis, tumours without necrosis showed higher oestrogen content than those with necrosis. The previous results may explain significant higher 2 year recurrent survival in cases with well differentiated tumours, little fibrosis and absent necrosis found in the present study

Evaluation of nucleolar organizer regions in Non-hodgkin's Lymphoma

46 lymph nodes specimens of N.H.L were included in the present study. Paraffin processed sections were prepared and stained with the following: 1. Hx. and E. for histopathological typing of N.H.L. according to the working formulation classification. 2-Argyrophil silver staining for analysis of NORs. Our results demonstrated there is well defined difference: between low, intermediate and 5 of grade lymphomas on the basis of NORs count of their cells.Also, NORs count increased in patient living than 2 years. Our findings documented the considerable value of the Ag-NORs for both grading and prognosis in NHL

Histopathological and histochemical, effects of snake venom and antivenom: an experimental study

The work is an attempt towards a better and clear understanding of the effect of snake venom [Ceriastes cerastes] on the different tissue of the experimental of the results occurring after the use of antivenom in the treatment of envenomation was done.The study includes the following groups : Group I: To demonstrate the effect of single different doses [0.5, 1.15 and 2 ug/gm body weight] of Cerastes venom The different organs taken [Liver, Kidneys, lungs and heart] showed marked histopathological and histochemical [alkaline phosphatase] changes which their degree being proportional according to the dose given. On in the other hand, the brain was the least sensitive of all the organs examined.Group II : To demonstrate the effect of repeated small doses [0.5 ug/gm, body weight] of Cerastes cerastes venom every other day for six doses on the different tissues of guineapigs. The same changes with group I were noticed but to a lesser extent. Group III: To demonstrate the effect of polyvalent antivenom after the venom.The toxic-induced changes were markedly reduced. It is to be concluded from this study that single different doses of Cerastes cerastes venom have both histopathological and histochemical effects on different tissue and the degree of these effects being proportional to the dose given. Repeated small doses have a lesser effect. It is recommended that the appropriate antivenom should be given to prevent or reduce the damaging effect of the venom

Characterization of nucleolar organizer regions [NORs] in benign and malignant epithelial breast lesions

This study was conducted on 36 benign and 36 malignant epithelial lesions.They were processed as paraffin sections and attained with: 1] Hx. and E. for : a-Histopathological typing of the different lesions b- Histopathological grading of the malignant lesions 2] Argyrophil silver colloid technique for NORs demonstration It has been shown that, in the present study, NORs can differentiate between benign and malignant lesions, a finding which facilitate the resolution of some well-known diagnostic problems in the breast. The rapidity of the method of staining, the ease of interpretation and being inexspensive add advantages to it. Moreover, NORs number in malignant lesions can provide an information about the proliferative status of the cells and so it can be used as a prognostic factor supplementary to other prognostic indices in breast carcinoma

Histopathological and histochemical studies on experimentally infected hamsters with stictodora tridactyla

Histopathological changes in the intestinal mucosa in the form of inflammation with atrophic changes in the villi and foci of cellular reaction were seen at the sites of experimental infection in hamsters with Stictodora tridactyla. The activity of succinic dehydrogenase, non specific esterase and alkaline phosphatase enzymes in the villi and the activity of acid phosphatase in the glands were markedly reduced. These changes suggest a disturbance in both digestive and absorptive mechanisms of the small intestine

Histopathology of cervix after injectable contraceptives

This study included 40 women aged between 25- 40 years. 20 of them were injected I.M. with Depoprovera 150mg every 3 months for 5 injections [15 months], and the other 20 were injected with Noresterat 200mg every 2 months for 7 injections [14 months]. Punch biopsy was taken from the cervix before and after the injections to show the histopathology of the cervix. With Depoprovera 3 cases of mild dysplasia, 2 cases of microglandular hyperplasia, and 2 cases of squamous metaplasia. In Noresterat users, one case of mild dysplasia, one case of microglandular hyperplasia, and 2 cases of squamous metaplasia