ABSTRACT
This study was conducted to know the influence of alcohol and aging on carbohydrate metabolic profiles in the kidney tissue of control and experimental male albino rats. Alcohol significantly decreased the levels of glucose, total proteins, total carbohydrates and increased free amino acids, pyruvate and lactate in the kidney tissue of both age groups of rats. Aging induced the elevation of the levels of glucose, total carbohydrates, free amino acids and lactate, while it decreased the total protein and pyruvate contents. This suggests rapid utilisation of carbohydrates and degradation of proteins to meet energy demands under alcoholic stress. Thus more alterations were observed in the carbohydrate metabolic profiles in kidney of both age groups of experimental rats.
ABSTRACT
Exercise together with chronic ingestion of ethanol produces physiological and morphological alterations in skeletal muscle. The present study has been carried out to investigate the combined effect of exercise and ethanol ingestion on selected energy metabolic profiles of skeletal muscle fibres with reference to age induced changes. Wistar strain albino rats of two age groups (3 months & 18 months) were divided into four groups - Group I, sedentary control (SC); Group II, exercise (ExT) (30 min, speed of 23 m/min/day/5 days/week for a period of 8 weeks); Group III, ethanol treated (Et) (20% ethanol, 2 gm/kg body weight); Group IV, exercise trained + ethanol treated (ExT + Et) as mentioned in Groups II and III. The animals were sacrificed after 24 hours of the last treatment by cervical dislocation, and the skeletal muscle fibres of gastrocnemius (GN) and soleus (SOL) were isolated from the hind limbs, and selected energy metabolic profiles such as carbohydrates, glycogen, and free amino acids were estimated. The total carbohydrate content, glycogen and FAA are significantly elevated with ExT and also with combination treatment. However, the same parameters were decreased with ethanol intoxication in both skeletal muscle fibres when compared with sedentary control rats. The results suggest a beneficial role of exercise in preventing ethanol-induced toxicity.