The measurement of pgam-b using elisa and changes of serum concentration by physical exercise / 体力科学

The present paper focuses on the leaking enzyme, PGAM (phosphoglycerate mutase), which is attracting increasing interest among medical researchers, and its relations with physical exercise. As a preliminary, purified PGAM-B was extracted from erythrocyte. First of all, erythrocytes were burst by hypotonic buffer. The contents were let through DEAE-Sepharose column and then through an affinity column bound with PGAM antibody. The purified substance thus obtained is analyzed by SDS-PAGE and has been found only in the single band of approximately 30 kDa. It has been injected to a mouse to develop its antibody. The mouse antibody thus developed and commercial anti r-h-PGAM-B rabbit antibody have been used to in order to perform double antibody sandwich ELISA on PGAM-B. R-h-PGAM-B was used as a standard substance. It has been observed that our standard curve shows a linearity in the range of 0-100ng/100μl, with R<SUP>2</SUP>=0.9849. The correla-tion between intensity of physical training and PGAM-B concentration in serum has been analyzed. Non-athlete male students, long distance male and female runners took part in the experiment. The sera of the male runners were found to have 34 times higher PGAM-B concentration than those of the non-athletes. The male runners had 3.6 times higher concentration than those of female runners. Non-athletes ran 10 km and their serum PGAM-B concentration were measured afterwards. It was found that concentration was 3.2 times higher after the run than before it, and this increasing found in all subjects.

The Charactaristic of H-shape sport's mouth guard on athlete's physical capacity. Part II Effect on the leg power and golf perfomance / 体力科学

We have developed a mouth guard which has revolutionized the shape and material of the conventional mouth piece. The H shape mouth guard has the unique shape like that of an H. Even with the new shape of the mouth piece it does not hinder the performance of the wearer. The following are the fundamental data collected by our group. In this study we examined its influences on leg power, the carry (the distance a golf ball is hit) and the motion of the club head during a tee off.<BR>The results are as follows:<BR>1) By wearing or setting the mouth guard, the variation of leg power, measured with a Sybex II, showed an increase at selected angular verocity. The rate of increase averaged aproximately 15%.<BR>2) The amount of work in anaerodush, an intensive, but short exercise, increased significantly by 1.25 seconds.<BR>3) As for golf, all four of the test subjects joint performance increased their carries by 5.2 to 11.6 yards, using a 1st wood.<BR>4) Also the fluctuation of the head of the club decreased from 7.3 cm to 3.6 cm on average.<BR>Our group have found that the new H shaped mouth guard can absorb shock as well as not hinder the performance of the individual who is wearing it. Thus, the H shape mouth guard can be worn during sports to enhance the participant's performance.

The characteristic of H-shape sport's mouth guard on athlete's physical capacity. Effects on shock absorbability and respiratory function / 体力科学

Generally, sport players of boxing or football who contact roughly with other players use mouth guard during match or game to prevent the breaking of jow boneor bursting of soft oral cavity. Moreover, mouth-guard are used for the purpose to lighter the load to root of teeth and to improve the skill and power of sport's player.<BR>The purpose of this study was to examine the charactaristic of unfitted by H shape-mouth guard compared to U shape mouth guard.<BR>The following results were obtained.<BR>1. The H shape-mouth guard of this study could reduce the impact to 1/7 level.<BR>2. A relative decrease in ventilation level was shown when H shape mouth guard fitted.<BR>3. With reference to respiratory function, reduction of the amount of oxygen up-take was not observed at heart rate lower than 170 beats/min, and in this case a player feells subjective symptom of stiffing.

Energy systems during the last spurt (exertion) in an 800-m race / 体力科学

A study was conducted to investigate the relationship between energy systems and running performance, especially during the last spurt in an 800-m race. The subjects were separated into good 800-m runners (group A n=5: best record, 1′54″3±1.4) and a second group of slower 800-m runners (group B n=4: best record, 2′02″1±1.3) . Each group executed two types of running test (600m test and 800m test) . To simulate an 800-m race, the running speed up to 600m was set by means of a lamp pace maker system. The last spurt was running 200m at maximal voluntary running speed. Plasma lactate, plasma ammonia, serum glucose and blood pH were assayed at rest, after warming up, and 6 and 10min after the running test.<BR>The following results were obtained:<BR>1) The last spurt time of group A was significantly faster than that of group B (p<0.01) . 2) In group A, plasma lactate and plasma ammonia concentrations increased significantly during the last spurt (p<0.05) . In group B, however, plasma lactate and plasma ammonia concentrations before the last spurt (600m test values) were very close to the values after the last spurt (800m test values), 3) Last spurt times were significantly related to changes in plasma lactate concentration (r=-0.870, p<0.01) and O<SUP>2</SUP> debt (r=-0.799, p<0.01) during the last spurt.<BR>These results suggest that running performance during the last spurt in an 800-m race depends on anaerobic energy ability, as reflected by plasma lactate, plasma ammonia and O<SUP>2</SUP> debt before the last spurt.

Analysis of human urinary proteins after physical exercise by two-dimensional electrophoresis under non-denaturing conditions / 体力科学

A study was conducted to investigate the changes in human urinary proteins caused by physical exercise. Nine subjects (male middle-distance runners, 19-21 years) were loaded physical exercise with a 10km-run, and quantitative and qualitative changes in urinary proteins were examined using various techniques including polyacrylamide gel electrophoresis, immunoelectrophoresis and two-dimensional electrophoresis. Total protein concentrations in urine from all subjects before exercise were very low (0.68-2.56mg/d<I>l</I>), but those were increased remarkably after exercise. Few proteins except for albumin were detected in urine before run by polyacrylamide gel electrophoresis and immunoelectrophoresis, however, a few proteins were observed after exercise in addition to a marked increase of albumin. Several proteins which were not observed in serum could also be detected in urine after exercise by two-dimensional electrophoresis under non-denaturing condition. It is considered that two-dimensional electrophoresis is a very powerful technique for analyzing dilute and complicated protein mixture in biological fluids such as urine.