ABSTRACT
Aim of this study was to determine the effect of diazepam on serum electrolytes [sodium, potassium, calcium, and magnesium], osmolarity and anxiolysis of 2hrs restraint stressed rats. Male Albino Wistar rats were used in experiment. Test animals were injected diazepam intrap-eritoneal and control deionized water. After thirty minutes of injection control and test animals were further divided into restrained and unrestrained groups. Animals of unrestrained group were kept in home cages, while restrained grouped animals were restrained by immobilizing for two hours [2hrs] on wire grids. Next day light-dark box activity was monitored for 5 minutes. Rats were decapitated. Blood was collected and serum separated for analysis of electrolytes. Results showed that diazepam increases the serum sodium, potassium, calcium, concentration significantly [p<0.01]. It also increases the osmolarity; number of events and time spent in light box but decreased magnesium concentration significantly [P<0.01] in stressed and unstressed rats. It is concluded that the diazepam reverse the stressed produced changes in serum electrolytes and plasma osmolarity
ABSTRACT
Aim of this study was to determine the effect of immobilization stress on serum electrolytes [sodium, potassium, calcium and magnesium] in rats. Male albino wistar rats were used in experiment. Test animals were restrained by immobilizing for two hours on wire grids. Control animals were kept unrestrained in home cages. Rats were killed after the termination of restraint period. Blood was collected and serum separated for the analysis of electrolytes. Serum sodium, potassium and calcium were analyzed by flame photometer. Magnesium content was determined by spectrophotometer. Result shows a significant decreased [P = 0.001] in sodium, potassium, and calcium but increased [P = 0.001] in magnesium serum level. It is concluded that stress induced decrease in sodium, potassium and calcium and increase in serum magnesium level may contribute to stress induce hypertension