ABSTRACT
<p><b>OBJECTIVE</b>To investigate the specific cell-mediated immune efficacy of the an HPV16 prophylactic vaccine.</p><p><b>METHODS</b>C57BL/6 mice were randomly divided into 3 groups: experimental group I (treated with pcDNA L1), control group II (treated with pcDNA3.1) and control group III (treated with PBS buffer). The mice were immunized three times during a three-week interval. Ten to fourteen days after the third inoculation, a footpad swelling test was used to detect delayed-type hypersensitivity (DTH) responses. Antigen-specific splenocyte proliferation assay and quantitation of IFN-gamma cells in splenocytes were performed by FACS assay.</p><p><b>RESULTS</b>In the experimental group, splenocytes actively proliferated after stimulation with HPV16 VLP, and had developed a markedly larger amount of CD8(+) IFN-gamma(+) cells, which is an index for special CTL. Also, the footpad was significantly thickened upon inoculation with HPV16 VLP.</p><p><b>CONCLUSION</b>Naked DNA vaccine of HPV16 L1 can induce specific cell-mediated immune responses in mice, which should be considered for evaluation of HPV16 DNA vaccine feasibility.</p>
Subject(s)
Animals , Mice , Hypersensitivity, Delayed , Immunization , Interferon-gamma , Lymphocyte Activation , Mice, Inbred C57BL , Papillomaviridae , Allergy and Immunology , Spleen , Cell Biology , Vaccines, DNA , Allergy and Immunology , Viral Vaccines , Allergy and ImmunologyABSTRACT
The TNA mRNA expression in 33 samples of cervical carcinoma and 28 samples of condyloma have been observed by using ~3H and Digoxigenin labeled TNF—? cDNA probes and in situ hybridization technique, we found that the results of in situ hybrdfzation with these two kinds of labeled probes were alike, the sensitivity with ~3H labeled probe was slightly higher than that with Digoxigenin labeled probe. Nevertheless, the nonisotope probe would be used more and more in future because of its safety, rapidity and convenience in work.