1.
Chinese Journal of Information on Traditional Chinese Medicine
;
(12)2006.
Article
in Chinese
| WPRIM
| ID: wpr-576566
ABSTRACT
Objective To study the stability of the Bradford method for determination of soluble protein in animal materials. Method The absorption of CBB G-250 combined with proteins was determined by the UV-Spectrophotometry method with the detection wavelength at 595 nm,and the dried bufo was selected as sample,BSA as control. Results The method showed a good linear relationship between absorption and protein content (r =0.996). The sample was stable with in 10 min (RSD=5.0%). Conclusion This method was reliable,simple and rapid.