ABSTRACT
Ferroptosis is a new type of programmed cell death that is closely associated with the pathophysiological process of ischemic stroke. Ferroptosis inhibitors can improve neurological function and provide neuroprotection after cerebral ischemia. Therefore, the role of ferroptosis in ischemic stroke and the regulation of ferroptosis to intervene in the occurrence and development of ischemic stroke have become a research hotspot. This article reviews the molecular mechanism and potential therapeutic targets of ferroptosis during ischemic stroke, hoping to provide new perspectives for the treatment of ischemic stroke.
ABSTRACT
Objective: To investigate the effects of miR-26a targeting high mobility group protein 1 (HMGA1) gene on the growth, invasion and migration of colon cancer cells, and to clarify whether the HMGA1 gene was the target gene of miR-26a. Methods: The miR-NC (miR-NC group), miR-26a mimics (miR-26a mimics group) and miR-26a inhibitor (miR-26a inhibitor group) were transfected into the human colon cancer SW480 cells. RT-PCR and Western blotting methods were used to detect the mRNA and protein expression levels of HMGA1. Luciferase reporter gene detection kit was used to detect the double luciferase activity in SW480 cells and to determine whether HMGA1 was the target gene of miR-26a. The cell proliferation activities of cells in various groups were detected by CCK8 assay; the number of invasion and migration cells was detected by Transwell chamber method. Results: HMGA1 gene was the target gene of miR-26a. Compared with miR-NC group, the proliferation activities of colon cancer SW480 cells in miR-26a mimics group at after 24, 48 and 72 h after transfection were significantly decreased (P<0. 01), while the proliferation activities of colon cancer SW480 cells in miR-26a inhibitor group were significantly increased (P<0. 01). Compared with miR-NC group, the proliferation activities of colon cancer SW480 cells, the number of invasion and migration cells in miR-26a mimics group and miR-26a mimics + pcDNA3. 1-HMGA1 group at different time points were significantly decreased (P<0. 01); the proliferation activities of colon cancer SW480 cells, the number of invasion and migration cells in miR-NC + pcDNA3. 1-HMGA1 group were increased (P<0. 01). Compared with miR-26a mimics group, the proliferation activities of colon cancer SW480 cells, the number of invasion and migration cells in miR-26a mimics+ pcDNA3. 1-HMGA1 group at different time points were increased (P<0. 01); compared with miR-NC+pcDNA3. 1-HMGA1 group, the proliferation activities of colon cancer SW480 cells, the number of invasion and migration cells in miR-26 mimics + pcDNA3. 1-HMGA1 group at different time points were decreased (P<0. 01). Conclusion: HMGA1 gene is the target gene of miR-26a. Up-regulation of the expression of miR-26a can inhibit the proliferation of colon cancer SW480 cells and downregulation of the expression of miR-26a can promote the proliferation of colon cancer SW480 cells.
ABSTRACT
Objective To investigate the efficacy of frontoparietal drill versus parietal drill in the burring hole and drainage for chronic subdural hematoma(CSDH),and provide an evidence for clinical application.Methods A total of 82 patients with CSDH from January 2013 to January 2016 in our hospital were randomly divided into observation group and control group.The observation group chose frontoparietal drill which located at the front 1/4 point at the axial view with the thickest hematoma.Control group chose parietal drill which nearby the parietal tuberodties at the axial view with the thickest hematoma.The postoperative residual hematoma volume,intracranial gas volume,recurrence rate,neurologic function and mental state between two groups were compared.Results The postoperative residual hematoma volume,intracranial gas volume,the recurrence rate and CSS were lower compared with the control group,the differences were significant(P<0.05).The MMSE score of observation group were higher that of the control group,the difference was significant(P<0.05).Conclusion The frontoparietal drill has a better effect to remove the hematoma and promotes neural functional recovery.
ABSTRACT
Objective To observe the correlation between CD64 index levels in peripheral blood and the severity and prognosis factors of postoperative traumatic sepsis,and investigate the clinical effect of these changes in the development of postoperative traumatic sepsis.Methods A total of 560 patients with trauma were enrolled in the study,and these patients were divided into the postoperative traumatic sepsis group (210 cases) and the postoperative general trauma group (350 cases) according to clinical manifestations.According to the severity of the postoperative traumatic sepsis,these patients were divided into low-risk group (64 cases),moderate-risk group (75 cases) and high-risk group (71 cases).According to clinical prognosis,these patients with post-traumatic sepsis were divided into survival group (178 cases) and death group (32 cases).The CD64 index in peripheral blood and APACHE Ⅱ score were analyzed and measured by flow cytometry.The differences and correlation between CD64 index levels and APACHE Ⅱ score were compared and analyzed,and the relationship between CD64 index levels and the severity and prognosis of postoperative traumatic sepsis were also assessed.Results The CD64 index levels of postoperative traumatic sepsis group were higher than the postoperative general trauma group (P<0.05).The differences in terms of CD64 index,APACHE Ⅱ score and mortality rate among low-risk group,moderate-risk group and high-risk group were statistically significant,in which high-risk group was the highest,followed by moderate-risk group,and low-risk group was the lowest(P<0.05).Compared with the survival group,the death group had higher CD64 index levels and APACHE Ⅱ score (P<0.05).The CD64 index levels were positive correlated with APACHE Ⅱ score (r=0.72,P=0.00) and mortality (r=0.56,P=0.00).ROC curves analysis results showed that the area under curve of CD64 index was 0.874 (95%CI:0.765~0.896), and the optimal operating point was 7.08,which had 82.6% sensiticity and 86.4% specificity.Conclusion The increased CD64 index levels in postoperative traumatic sepsis are closely related to the severity and prognosis of disease,and the CD64 index levels can be effective predictor with some clinical application value.
ABSTRACT
Objective To explore the clinical efficacy of dynamic hip screw( DHS) and proximal femoral nail anti-rotation( PFNA) in treatment of patients with Parkinson’ s disease and intertrochanteric fracture. Methods A total of 62 elderly patients of Parkinson’ s disease with femoral intertrochanteric fracture in our hospital from February 2010 to February 2014 were divided into two groups according to different internal fixations,with 31 cases in DHS group and PFNA group respectively. The operation time,X-ray fluoroscopy times,intraoperatve blood soss,the healing time of fracture,postoperative complications and Harris score between two groups were recorded and compared statistically. Results The operation time,intraoperatve blood soss and the clinical healing time of PFNA group were significantly lower than those of DHS group,the differences were statistically significant (P0. 05). Conclusion The PFNA has the advantages of shorter operation time,less bleed-ing,faster healing time in treatment for elderly patients with Parkinson’ s disease and intertrochanteric fracture,worth clinical promotion.