ABSTRACT
Objective@#To explore the effectiveness of exercise combined with nutrition intervention on body mass index (BMI), blood glucose and blood lipid in obese adolescents.@*Methods@#Eighty obese adolescents who participated in a weight-loss training camp were selected as the intervention group, and 50 healthy adolescents were selected as the control group. Adolescents in the intervention group received 8-week weight-loss training and nutritional intervention, while those in the control group maintained their original lifestyle. The changes in body weight, body fat percentage, blood glucose, lipid and inflammatory indicators were compared before and after intervention in both groups.@*Results@#After intervention, weight, BMI, body fat percentage and waisthip ratio of the intervention group were significantly lower than those before intervention (P<0.05). The levels of insulin, HOMA-IR, TG and LDL in the intervention group before intervention were significantly higher than those in the control group (P<0.05). There was no significant difference in blood glucose after intervention (P>0.05). The levels of insulin, HOMA-IR, TG and LDL after intervention were significantly lower than those before intervention (P<0.05). There was no significant difference in HOMA-IR, TG and LDL levels between the intervention and the control group (P>0.05). The levels of TNF-α and hs-CRP in the intervention group before intervention were significantly higher than those in the control group (P<0.05); the levels of TNF-α and hs-CRP in the study group after intervention were significantly lower than those before intervention(P<0.05); the levels of TNF-α and hs-CRP in the control group before and after intervention had no significant changes (P>0.05).@*Conclusion@#Exercise combined with nutrition intervention can effectively reduce body fat and body weight, improve insulin resistance, regulate glycolipid metabolism and reduce inflammation in adolescent obese patients.
ABSTRACT
BACKGROUND: Human leukocyte antigen-G (HLA-G) is a tumor-associated molecule, whose expression may help the cancer cells to escape the immune response. AIMS: The aim of this study was to evaluate the diagnostic value of HLA-G level in oral squamous cell carcinoma (OSCC). MATERIALS AND METHODS: A total of 52 patients who had definite pathological diagnosis and 20 cases of healthy controls were enrolled in this clinical trial. Immunohistochemisty (IHC) and quantitative real-time reverse transcription-polymerase chain reaction (RT-PCR) analysis were considered for HLA-G identification and multilevel validations. Statistical analysis was performed using SPSS and statistical significance was determined at P < 0.05. RESULTS: IHC results demonstrated that the expression of HLA-G in OSCC was strongly positive and the rate of positive expression was 55.77% (29/52), but the expression of HLA-G in healthy controls was negative (0/20). Furthermore, RT-PCR results showed that the positive expression rate of HLA-G messenger RNA was weak in healthy controls, but strong in OSCC. Besides, HLA-G expression in the tumors was significantly correlated with histological grade. CONCLUSIONS: Our results suggested that HLA-G is associated with the prognosis of OSCC and may serve as a novel therapeutic target.