Hymenaea rubriflora Ducke stem bark extract has vasorelaxant and contractile inhibition capacity

Abstract We investigated the vasodilatory effects of Hymenaea rubriflora Ducke stem bark extract (HR- HAc). Vascular reactivity of the aortic rings of Wistar rats was tested by in vitro cumulative doses (0.1 - 729 μg/mL). Rats (n=5) were treated with 25 (G25), 50 (G50) and 100 (G100) mg/ kg of HR-HAc or saline (control group - CG) for four weeks. An in vitro assay resulted in dose-dependent relaxation of the aortic rings with functional endothelium, which was inhibited in the presence of L-NAME. Rings of the treated animals increased acetylcholine relaxing potency at all doses, with a greater effect on G50 (pD2 = 7.8±0.1, Emax = 95.6±1.1) and a decreased contractile potency to phenylephrine in G25 (pD2 = 6.9±0.06, Emax = 61.5±6.0%) and G50 (pD2= 6.6±0.06, Emax = 71.0±8.5%) when compared to the CG in the presence and absence of endothelium (pD2= 6.4± 0.1, 6.4±0.1 and 6.9±0.1, respectively). Cumulative doses of nitroprusside resulted in increased relaxing potency in all treated groups and maintained Emax at 100%. It is concluded that HR-HAc has vasorelaxant capacity and inhibitory vascular contraction activity applied either directly to aortic rings or after treatment with in vivo supplementation, which places this extract as a potential nutraceutical or pharmacological agent for treating diseases associated with vascular dysfunction.

SUPLEMENTAÇÃO COM MUCUNA PRURIENS ASSOCIADA AO TREINAMENTO DE FORÇA NÃO ALTERA CONSUMO ALIMENTAR, PESO CORPORAL E MEDIDAS MURINOMÉTRICAS EM RATOS TREINADOS / MUCUNA PRURIENS SUPPLEMENTATION ASSOCIATED WITH RESISTANCE TRAINING DOES NOT CHANGE FOOD INTAKE, BODY WEIGHT AND MURINOMETRIC VALUES IN TRAINED ANIMALS

RESUMOMucuna pruriens (MP) tem sido comercializada como suplemento para emagrecimento mesmo sem comprovação científica. Este estudo objetiva avaliar o efeito da MP associada ao treinamento resistido sobre parâmetros murinométricos e consumo alimentar em ratos. Os animais foram randomizados nos grupos: controle sedentário e treinado, MP sedentário e treinado. Os treinados executaram protocolo de saltos e os suplementados receberam 250 mg de MP/kg/dia via gavagem. O consumo alimentar e peso corporal foram avaliados semanalmente e medidas murinométricas ao final do protocolo. Não houve alterações no consumo alimentar (147 a 180 g), peso (341 a 368 g), comprimento (entre 24,6 e 25,5 cm), circunferências torácica (de 14,1 a 16,2 cm) e abdominal (variação de 14,5 a 15,8 cm), índice de massa corporal (entre 0,53 e 0,56 g/cm2) e índice de Lee (entre 4,5 e 4,8 cm3). Suplementação de MP associada ao exercício não altera consumo alimentar, peso e medidas murinométricas em ratos.

ABSTRACTMucuna pruriens(mp) has been marketed as a supplement for weight loss even without scientific proof. this study aims to evaluate the effect of mp supplementation associated with resistance training on murinometric parameters and food consumption in rats. animals were randomized into sedentary and control groups, supplemented with mp or not. trained animals performed the jump protocol and received the mp supplementation (250 mg/kg/day) by gavage. food consumption and body weight were assessed weekly and murinometric parameters, at the end of the protocol. there were no changes in food intake (147 - 180 g), weight (341 - 368 g), length (24.6 - 25.5 cm), chest (14.1 - 16.2 cm) and abdominal circumferences (14.5 - 15.8 cm), body mass index (0.53 - 0.56 g/cm2) and lee index (4.5 - 4.8 g/cm3). mp supplementation during exercise does not alter food intake, weight and murinometric measurements in rats.

Treinamento de força reduz perfil glicêmico de ratos / Resistance training reduces blood glucose in rats

Aim: To evaluate the effect of a ten-week strength training on the blood glucose of rats. Methods: Eighteen rats were divided into two groups: sedentary and trained. Trained animals underwent strength training for ten weeks. The animals performed four sets of eight jumps with intervals of 30 seconds during ten weeks with a frequency of 3 times per week. An overload weight was imposed to the animals, using appropriate vests, which increased every two weeks. Body weight and food consumption were evaluated weekly. At the end of the experiment, the animals were euthanized and theirblood glucose and weights of visceral fat and carcass were measured. Results: No changes were observed in body weight (359.29±38.56 g vs. 355±15.58 g), food intake (162.1±16.6 g vs 156.57±5.4 g) and weight of carcass (222.5±22.7 g vs 230.7±9.3 g), however, reduction of visceral fat (17.4±3.3 g vs 12.9±1.7 g) and blood glucose (180.0±21.9 mg/dL vs. 151.7±19.6 mg/dL) was observed in the trained animals. Conclusion: The protocol used in this study reduced visceral fat and blood glucose in animals subjected to strength training.

Objetivo: Avaliar o efeito de um treinamento de força de dez semanas sobre o perfil glicídico de ratos. Métodos: 18 ratos foram randomizados em dois grupos: sedentários e treinados. Os animais treinados foram submetidos a treinamento de força durante dez semanas. Os animais realizavam quatro séries de oito saltos com intervalos de 30 segundos, durante dez semanas, com frequência de três vezes por semana. Foi imposta uma sobrecarga de peso aos animais, utilizando-se coletes apropriados, aumentada quinzenalmente. Foram avaliados peso corporal e consumo alimentar semanalmente. Ao final do experimento, os animais foram eutanasiados e verificou-se glicemia, peso de gordura visceral e de carcaça. Resultados: Não foram observadas alterações no peso corporal (359,29 ± 38,56 g vs. 355 ± 15,58 g), consumo alimentar (162,1 ± 16,6 g vs. 156,57 ± 5,4 g) e peso da carcaça (222,5 ± 22,7 g vs. 230,7 ± 9,3 g), entretanto, encontrou-se redução da gordura visceral (17,4 ± 3,3 g vs. 12,9 ± 1,7 g) e da glicemia (180,0 ± 21,9 mg/dL vs. 151,7±19,6 mg/dL) nos animais treinados. Conclusão: O protocolo aplicado neste estudo reduziu gordura visceral e glicemia nos animais submetidos ao treinamento de força.