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Objective: To investigate protective effects of Hydnophytum formicarum Jack. (H. formicarum) extracts via regulation of SIRT1-FOXO3a-ADAM10 signaling and antioxidant activity against H
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Objective:To investigate protective effects of Hydnophytum formicarum Jack. (H. formicarum) extracts via regulation of SIRT1-FOXO3a-ADAM10 signaling and antioxidant activity against HMethods:Cell viability and apoptosis of neuronal cells pretreated with H. formicarum Jack. extracts under oxidative stress were determined by MTT assay and flow cytometry. The intracellular reactive oxygen species (ROS) was performed using Carboxy-DCFDA assay. Additionally, a profile of protein expressions related to neuroprotection was detected by western blot analysis.Results:The plant extracts (methanol and ethyl acetate) elicited protective effects on the neuronal cell death as performed by the MTT assay and by apoptosis analysis via the activation of BCL-2. Both ethyl acetate and methanol extracts exerted inhibitory effects against HConclusions:The recent findings suggest the protective effects of H. formicarum Jack. plant extracts on attenuating H
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Objective To investigate protective effects of Spilanthes acmella (S. acmella) Murr. extracts against pesticide-induced neuronal cells death and to elucidate the underlying molecular mechanism in dopaminergic (SH-SY5Y) cells lines. Methods Cell viability of SH-SY5Y cells was studied by treating the cells with various concentration of pirimicarb for 24 h. Neuroprotective effect of S. acmella Murr. extracts was investigated by adding the plant extracts to the medium for 24 h prior to the incubation with 100 μM H
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OBJECTIVE@#To investigate protective effects of Spilanthes acmella (S. acmella) Murr. extracts against pesticide-induced neuronal cells death and to elucidate the underlying molecular mechanism in dopaminergic (SH-SY5Y) cells lines.@*METHODS@#Cell viability of SH-SY5Y cells was studied by treating the cells with various concentration of pirimicarb for 24 h. Neuroprotective effect of S. acmella Murr. extracts was investigated by adding the plant extracts to the medium for 24 h prior to the incubation with 100 μM HO or with pirimicarb for 24 h. Control-untreated cells were incubated with the culture medium. Cell viability was measured by MTT assay, calpain and calpastatin expressions were analyzed by Western blotting and immunocytochemistry.@*RESULTS@#Pretreatment of SH-SY5Y cells with S. acmella Murr. extracts (1 μg/mL) for 24 h significantly increased the dopaminergic neurons in pirimicarb-induced neurotoxicity. In addition, pretreatment with the S. acmella Murr. extracts led to decreased calpain but increased calpastatin protein levels.@*CONCLUSION@#S. acmella Murr. extracts exerted neuroprotective effect, via an alteration of calcium homeostasis, against pirimicarb induced neurotoxicity. The S. acmella Murr. might be a potential natural candidate with neuroprotective activity.
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Background: Human Cu/Zn superoxide dismutase [hSOD1] is an antioxidant enzyme with potential as a therapeutic agent. However, heterologous expression of hSOD1 has remained an issue due to Cu[2+] insufficiency at protein active site, leading to low solubility and enzymatic activity
Objectives: The effect of co-expressed human copper chaperone [hCCS] to enhance the solubility and enzymatic activity of hSOD1 in E. coli was investigated in the presence and absence of Cu[2+]
Materials and Methods: pETDuet-1-hSOD1 and pETDuet-1-hCCS-hSOD1 were constructed and individually transformed into E. coli strain BL21[DE3]. The recombinant hSOD1 was expressed and purified using immobilized metal affinity chromatography. The yield and specific activity of hSOD1 in all conditions were studied
Results: Co-expression with hCCS increased hSOD1 solubility at 37[degree]C, but this effect was not observed at 25[degree]C. Notably, the specific activity of hSOD1 was enhanced by 1.5 fold and greater than 3 fold when co-expressed with hCCS at 25[degree]C with and without Cu[2+] supplement, respectively. However, the chaperone co-expression did not significantly increase the yield of hSOD1 comparable to the expression of hSOD1 alone
Conclusions: This study is the first report demonstrating a potential use of hCCS for heterologous production of hSOD1 with high enzymatic activity
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Objective: To investigate antioxidant and antimicrobial activities of Saraca thaipingensis Cantley ex Prain; and isolation of its flower extracts. Methods: The plant species (flowers, leaves, and twigs) were extracted by hexane, dichloromethane, ethyl acetate and methanol; and tested for antioxidant activity (DPPH assay) and antimicrobial activity (agar dilution method) against twenty-seven strains of microorganisms; gram positive and gram negative bacteria, and diploid fungus. Bioactive constituents were isolated by column chromatography. Results: The plant extracts has been firstly reported to display strong antioxidant activity and antimicrobial activity selective against gram positive bacteria (Corynebacterium diphtheriae NCTC 10356 and Streptococcus pyogenes) with MIC of 256 μg/mL. Stigmasterol and a mixture of triterpenoids and phenolic compounds were isolated from the flower extracts. Conclusions: The study revealed that the S. thaipingensis is a new source of natural antioxidants and antimicrobials with potential for medicinal uses.