Effect of restraint stress on hormonal levels, some blood constituents and immunological response of rams

10 rams were used to evaluate the effect of restraint stress on plasma levels of cortisol, ACTH and changes in some blood constituents as glucose, urea, creatinine, total proteins, albumen, globulin, GOT and GPT as well as immunological response including total, differential leukocytic count and lymphocytes blastogenic response to nitrogen. The animals were divided into two groups: The first [G I] unstressed served as control and the second [G II] was restrained by securing their legs with tape for 5 hours daily on 3 successive days. Blood samples were taken at 0 time, 5 hours and 10 hours. The results indicated significant increase in cortisol and ACTH which parallel with the time of exposure reaching maximum levels at 5 hours, significant increase in glucose, GOT and GPT, especially at 5 hours. Total protein, albumen and urea were significantly decreased, while creatinine and globulin fractions were not changed. Total leukocytic count showed significant increase at 5 and 10 hours which was more clear in 3rd day of exposure to stress. Significant increase in neutrophils, eosinophils appeared and significant decrease in lymphocytes number starting at 0 time, decreasing more at 5 hours especially 3rd day of the experiment. Nonsignificant changes appeared on monocytes number. The lymphocytes blastogenic response to mitogen showed that phytohemoagglutinin [PHA] increased significantly at 0 time reaching highest level at 5 hours, then decreased slightly at 10 hours but was higher than control. Concavalin A [Con. A] decreased significantly at 5 and 10 hours, especially after 3 days, Pokeweed [PW] decreased significantly at 0 time reaching lower level at 5 hours, then increased at 10 hours reaching control level

Effect of bromocriptine [parlodel] on hormonal profile of the adult male albino rats with special reference to changes in histologieal structure of the testes

Bromocriptine [Parlodel] is used for treatment of hyperprolactinemia in men, prolactin related hypogonadism [oligospermia-loss of libido, impotence]. It acts as stimulator for dopamine receptor. In this experiment, 60 adult male albino rats were divided into 3 equal groups. The first kept as control, injected with normal saline. Both the second and third groups were injected once daily with intramuscular [I/M] doses of 10 and 20 mug of bromocriptine dissolved in 0.4 ml of distilled water, respectively. Blood samples were collected after one and two weeks from treatment and plasma were separated and stored at -20C for hormone assay using RIA technique. The results indicated significant increase in testes weight, decrease in plasma levels of prolactin and testicular histamine concentration; while LH, FSH and testosterone were increased significantly with increasing the dose and time of treatment. The histological findings of the testes revealed changes including edema, exploration of seminiferous epithelium which increased after administration of the larger dose [20 mug] for two weeks