ABSTRACT
Objective: To prepare nerve growth factor(NGF) temperature sensitive in situ gel and investigate its therapeutic effect on sciatic nerve injury of rats.Method: NGF thermosensitive gel was prepared and its prescription was optimized by central composite design-response surface methodology.Fifty rats were randomly divided into the normal group,model group,NGF injection group(10 mg·L-1),NGF low-dose(10 mg·L-1) and high-dose(20 mg·L-1) thermosensitive gel groups,and sciatic nerve injury model of rats was established.The effect of NGF thermosensitive gel on the injury of sciatic nerve were comprehensively examined by taking rat behavior,sciatic nerve function index(SFI),time of withdrawal reflex,wet weight ratio of gastrocnemius muscle,and histomorphological changes as indicators.Result: The gelation temperature of NGF thermosensitive gel was 35.2℃ after the formulation being optimized,which was in line with the standard for injection.Four-eight weeks after operation,the SFI and wet weight ratio of gastrocnemius muscle in rats of NGF high-dose thermosensitive gel group were significantly higher than those in the model group and NGF injection group,but its time of withdrawal reflex was significantly lower than those in the model group and NGF injection group,and the effect was in a dose-dependent manner.Arrangement of regenerated nerve fibers in sciatic nerve injury area of rats from NGF high-dose thermosensitive gel group was more tidy,dense and continuous than that of the model group.Conclusion: NGF thermosensitive gel can promote repair of sciatic nerve injury in rats.
ABSTRACT
Objective To study the protective effect of rose (Rosae Rugosae Flos) essential oil on acute myocardial infarction in mice, and explore its mechanism. Methods The model of acute myocardial ischemia in mice was established by intraperitoneal injection of isopropyl adrenaline. The myocardial tissue pathological changes and the degree of myocardial injury in mice were observed by HE staining and TTC staining. Activities of super oxide dismutase (SOD) and glutathione peroxidase (GSH-Px) in myocardial tissue of mice were determined, and activities of creatine kinase isoenzyme (CK-MB) and lactate dehydrogenase (LDH) in serum of mice were determined as well. Results HE staining results showed that rose essential oil group (160, 80, and 40 mg/kg) can significantly improve myocardial tissue pathological injury in mice; TTC staining results showed rose essential oil (160, 80, and 40 mg/kg) can significantly reduce the myocardial ischemia areas in mice. Compared with model group, SOD and GSH-Px in the myocardial tissue of mice significantly increased (P < 0.05, 0.01), while enzyme activity of serum LDH and CK-MB significantly reduced in rose essential oil group (P < 0.05, 0.01). Conclusion Rose essential oil has certain protective effect on acute myocardial infarction injury and its mechanism may be associated with inhibiting oxidative damage, enhancing activities of anti-oxidant enzymes, and reducing the lipid peroxidation damage.