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Southeast Asian J Trop Med Public Health ; 2007 Jan; 38(1): 97-103
Article in English | IMSEAR | ID: sea-34410

ABSTRACT

Twenty-five clinical isolates of Leptospira spp were characterized by microscopic agglutination test (MAT) and pulsed field gel-electrophoresis (PFGE) in comparison with 23 reference Leptospira serovars and with the saprophytic L. biflexa serovar Patoc. PFGE DNA profiling was more specific and reliable than MAT.


Subject(s)
Agglutination Tests , Bacterial Typing Techniques , DNA, Bacterial/genetics , Electrophoresis, Gel, Pulsed-Field , Humans , Leptospira/classification , Leptospira interrogans/genetics , Leptospirosis/diagnosis , Reproducibility of Results , Sensitivity and Specificity , Serotyping , Thailand
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