ABSTRACT
OBJECTIVE@#To compare the therapeutic effects of low-temperature plasm radiofrequency and high-intensity focused ultrasound in treatment of perennial allergic rhinitis (PAR) complicated with nasal septum deviation.@*METHOD@#A total of 224 cases of identified PAR who treated in our hospital were randomly divided into 2 matched groups: low-temperature plasm radiofrequency group (n = 140) and high-intensity focused ultrasound group (n = 84). The therapeutic affection were evaluated by clinical symptoms controlled counting-scores. Inferior turbinate mucosa were collected and examined pathologically before and at 15 days and 6 months after the therapy.@*RESULT@#The follow-up data indicated the effective rate in 15 days was 97.1% in high-intensity focused ultrasound group, and 90.4% in low-temperature plasm radiofrequency group, in 6 months was 90.7% and 85.4% respectively.@*CONCLUSION@#The treatment of high-intensity focused ultrasound in PAR is minimally invasive and safe with reliable efficacy.
Subject(s)
Adolescent , Adult , Aged , Humans , Middle Aged , Young Adult , Catheter Ablation , Methods , Hypothermia, Induced , Nasal Septum , Congenital Abnormalities , Rhinitis, Allergic , Rhinitis, Allergic, Perennial , Therapeutics , Ultrasonic Therapy , MethodsABSTRACT
OBJECTIVE@#To study the differentiation of Th1/Th2 on the levels of transcription factors and cytokines production in patients with laryngeal squamous cell carcinoma in peripheral blood mononuclear cells (PBMC). In addition, the relation of p53 and T-bet, GATA3 expression was discussed for understanding the role of T-bet and GATA3 in metastasis.@*METHOD@#The gene expression of Th1/Th2 type transcription factors T-bet, GATA3 and cytokines IFN-gamma, IL-4 were determined by RT-PCR and realtime RT-PCR from 49 patients with laryngeal squamous cell carcinoma and 30 normal controls. The expression of p53 was analyzed by immunohistochemistry.@*RESULT@#Predominant expression of Th2 type cytokines was detected in 49 laryngeal squamous cell carcinoma patients. The expression rates of T-bet, GATA3, IFN-gamma and IL-4 was 42.86% (21/49), 71.43% (35/49), 26.53% (13/49), 63.27% (33/49) respectively. The expression rates of T-bet and IFN-gamma in patients were lower than in control group (P < 0.05), but the results of GATA3 and IL-4 were opposite (P < 0.05). The similar results were obtained by realtime PCR. The expression of p53 in patients was accompanied with lower expression of T-betmRNA and higher expression of GATA3mRNA.@*CONCLUSION@#There is predominant expression of Th2 type transcription factors and cytokines in PBMC of laryngeal squamous cell carcinoma patients. T-bet and GATA3 can be used as reference indicators for the metastasis of laryngeal squamous cell carcinoma.
Subject(s)
Adult , Aged , Female , Humans , Male , Middle Aged , Carcinoma, Squamous Cell , Metabolism , Pathology , Case-Control Studies , GATA3 Transcription Factor , Metabolism , Interferon-gamma , Metabolism , Interleukin-4 , Metabolism , Laryngeal Neoplasms , Metabolism , Pathology , Leukocytes, Mononuclear , Metabolism , RNA, Messenger , Genetics , T-Box Domain Proteins , Metabolism , Th1 Cells , Metabolism , Th2 Cells , Metabolism , Tumor Suppressor Protein p53 , MetabolismABSTRACT
OBJECTIVE@#To investigate the preventive effects of Pueraria on presbycusis in rats.@*METHOD@#Thirty-two 24-26 month old Wistar rats were randomly divided into four groups, and were treated with different dosages of Pueraria (1, 2, 4, 0 g x kg(-1) x d(-1)) separately for 4 weeks. Auditory brainstem response (ABR) was used to detect the change of hearing threshold of rats. Hemorheological items of rats were checked in each group.@*RESULT@#Compared with control group, the hearing threshold and hemorheological items of rats was significantly improved after treated with Pueraria (P<0.05). In addition, 2 g/(kg x d) was found to be the best dosage of Pueraria for rats, which can achieve ideal effect with minimum side effect.@*CONCLUSION@#Pueraria could improve tiny circulation, has good preventive effect on presbycusis of rats.
Subject(s)
Animals , Rats , Aging , Auditory Threshold , Evoked Potentials, Auditory, Brain Stem , Hemorheology , Phytotherapy , Presbycusis , Drug Therapy , Pueraria , Chemistry , Rats, WistarABSTRACT
Objective:To investigate the preventive effects of Pueraria on presbycusis in rats. Method:Thirty-two 24-26 month old Wistar rats were randomly divided into four groups, and were treated with different dosages of Pueraria (1,2,4,0 g·kg~(-1)·d~(-1)) seperately for 4 weeks. Auditory brainstem response (ABR) was used to de-tect the change of hearing threshold of rats. Hemorheological items of rats were checked in each group. Result: Compared with control group,the hearing threshold and hemorheological items of rats was significantly improved after treated with Pueraria(P<0. 05). In addition, 2 g/(kg·d) was found to be the best dosage of Pueraria for rats, which can achieve ideal effect with minimum side effect. Conclusion:Pueraria could improve tiny circulation, has good preventive effect on presbycusis of rats.
ABSTRACT
OBJECTIVE@#To investigate the expression of COX-2 in both molecule and protein levels in laryngeal squamous cell carcinoma and the relationship between its intensity and histological grade, clinical stage and lymphoma metastasis.@*METHOD@#By using emi-quantify RT-PCR and Immunohistochemistry, the expression of the COX-2 mRNA and COX-2 protein in laryngeal squamous cell carcinoma and polyp of vocal cord were examined.@*RESULT@#Compared with the polyp of vocal cord tissue, the expression level of COX-2 mRNA and COX-2 protein in human laryngeal squamous cell carcinoma were significantly increased (P < 0.01). The intensity of COX-2 mRNA and protein expression increased as the laryngeal squamous cell carcinoma progresses developed.@*CONCLUSION@#COX-2 is high expressed in human laryngeal squamous cell carcinoma, and it may play an significant role in the growth, invasion and metastasis of human laryngeal squamous cell carcinoma. A combination of emi-quantify RT-PCR and immunohistochemistry is sensitive and specific for laryngeal neoplasms detection.